Chronic effects of wastewater-borne silver and titanium dioxide nanoparticles on the rainbow trout (Oncorhynchus mykiss)

Zeumer, Richard; Galhano, Victor; Monteiro, Marta S.; Kuehr, Sebastian; Knopf, B; Meisterjahn, Boris; Soares, Amadeu M.V.M.; Loureiro, Susana; Lopes, Isabel; Schlechtriem, Christian

doi:10.1016/j.scitotenv.2020.137974

Cited by 35 publications

(18 citation statements)

References 67 publications

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“…However, it is not possible to have an actual estimation due to the uncontrolled use and release of NPs. Since aquatic environments are believed to be the ultimate receiver of these engineered materials, it is, therefore, important to evaluate the impacts of NPs on fish and other aquatic animals [26,27]. Fish can be also exposed to concentrations of NPs that are greater than environmental ones via food and the process of biomagnification through the food chain.…”

Section: Introductionmentioning

confidence: 99%

Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus

Rashidian

Lazado

Mahboub

et al. 2021

IJMS

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This study was conducted to compare the effects of commercially available (C) and green synthesized (GS) Zinc oxide nanoparticles (ZnO-NPs) on immunological responses of common carp (Cyprinus carpio) skin mucus. GS ZnO-NPs were generated using Thymus pubescent and characterized by UV–vis diffuse reflectance spectroscopy (DRS), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). Fish (n = 150) were randomly allocated into five groups in triplicate and received a waterborne concentration of 0% (control), 25%, and 50% of LC50 96 h of commercially available (C1 and C2) and green synthesized ZnO-NPs (GS1 and GS2) for 21 days. Results from XRD displayed ZnO-NPs with 58 nm in size and UV-vis DRS, EDX, and FT-IR analysis showed that some functional groups from plant extract bonded to the surface of NPs. The SEM images showed that ZnO-NPs have conical morphology. Acute toxicity study showed a higher dose of LC5096h for green synthesized ZnO-NPs (78.9 mg.L−1) compared to the commercial source (59.95 mg.L−1). The highest activity of lysozyme and alternative complement activity (ACH50) were found in control and GS1 groups. A significant decrease in alkaline phosphatase activity (ALP) was found in C1 and C2 groups compared to other treatments. Protease activity (P) was significantly decreased in the C2 group compared to the control and GS groups. Total immunoglobulin (total Ig) content was the highest in the control. In addition, total Ig in the GS1 group was higher than GS2. The exposure to ZnO-NPs lowered total protein content in all experimental groups when compared to control. Present findings revealed lower induced immunosuppressive effects by green synthesized ZnO-NPs on key parameters of fish skin mucus.

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Section: Introductionmentioning

confidence: 99%

Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus

Rashidian

Lazado

Mahboub

et al. 2021

IJMS

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“…On the contrary, waterborne exposure of the freshwater fish Carassius auratus to 200 µg L −1 of BP-3 promoted the increase in the activity of AChE [84]. Moreover, enhancement of the activity of AChE was also demonstrated in the brain of zebrafish exposed to an effluent supplemented with TiO 2 NPs, reinforcing that, when this NP is evaluated in mixture (natural occurring mixtures), its neurotoxicity can be promoted [83].…”

Section: Neurotoxicitymentioning

confidence: 81%

“…In the present study, no neurological disturbances of the cholinergic system were found in the brain after IP injection of TiO 2 NPs and BP-3, isolated or in mixture, for 3 and 7 days. In the same direction, several studies with freshwater fish reported no alterations in the AChE activity in the brain after waterborne exposure to TiO 2 NPs [46,[80][81][82][83]. On the contrary, waterborne exposure of the freshwater fish Carassius auratus to 200 µg L −1 of BP-3 promoted the increase in the activity of AChE [84].…”

Section: Neurotoxicitymentioning

confidence: 92%

Mild Effects of Sunscreen Agents on a Marine Flatfish: Oxidative Stress, Energetic Profiles, Neurotoxicity and Behaviour in Response to Titanium Dioxide Nanoparticles and Oxybenzone

Carvalhais

Pereira

Sabato

et al. 2021

IJMS

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UV filters are potentially harmful to marine organisms. Given their worldwide dissemination and the scarcity of studies on marine fish, we evaluated the toxicity of an organic (oxybenzone) and an inorganic (titanium dioxide nanoparticles) UV filter, individually and in a binary mixture, in the turbot (Scophthalmus maximus). Fish were intraperitoneally injected and a multi-level assessment was carried out 3 and 7 days later. Oxybenzone and titanium dioxide nanoparticles induced mild effects on turbot, both isolated and in mixture. Neither oxidative stress (intestine, liver and kidney) nor neurotoxicity (brain) was found. However, liver metabolic function was altered after 7 days, suggesting the impairment of the aerobic metabolism. An increased motility rate in oxybenzone treatment was the only behavioural alteration (day 7). The intestine and liver were preferentially targeted, while kidney and brain were unaffected. Both infra- and supra-additive interactions were perceived, with a toxicodynamic nature, resulting either in favourable or unfavourable toxicological outcomes, which were markedly dependent on the organ, parameter and post-injection time. The combined exposure to the UV filters did not show a consistent increment in toxicity in comparison with the isolated exposures, which is an ecologically relevant finding providing key information towards the formulation of environmentally safe sunscreen products.

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“…In Cl --rich media, oxidative dissolution is followed by the formation of different AgCl species, which have been shown to remain bioavailable to aquatic organisms 15 . A study using CuO NPs showed that transformation within a laboratory septic tank led to decreased toxicity in the zebrafish embryo hatching assay due to formation of non-bioavailable Cu species 10 while a decreased bioavailability and toxicity of wastewater-borne Ag NPs was previously shown for rainbow trout 16 . In contrast, effluents from Ag NP-spiked lab-scale sewage treatment plant exhibited higher toxicity to zebrafish embryos than the pristine NPs 11 .…”

Section: Introductionmentioning

confidence: 99%

Wastewater-Aged Silver Nanoparticles in Single and Combined Exposures with Titanium Dioxide Affect the Early Development of the Marine Copepod Tisbe battagliai

Georgantzopoulou

Farkas

Ndungù

et al. 2020

Environ. Sci. Technol.

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In this study, the effects of aged Ag and TiO 2 NPs, individually and as a mixture, in wastewater relative to their pristine counterparts on the development of the copepod nauplii (Tisbe battagliai) were investigated. NP behavior in synthetic wastewater and seawater was characterized during aging and exposure. A delayed development and subsequent mortality was observed after 6 days of exposure to aged Ag NPs, with a 2-fold decrease in EC50 ( 316µg/L) compared to pristine NPs (EC50 640 µg/L) despite the similar dissolved Ag concentrations measured for aged and pristine Ag NPs (441 µg/L and 378 µg/L, respectively).In co-exposures with TiO 2 NPs, higher dissolved Ag levels were measured for aged NPs (238.3 µg/L) relative to pristine NPs (98.57 µg/L). Co-exposure resulted in a slight decrease (15%) in the Ag NP EC50 (270 µg/L) with a 1.9-fold increase in Ag NP retained within the organisms after depuration (2.82% retention) compared to Ag NP single exposures as measured with sp-ICP-MS suggesting that the particles are still bioavailable despite the heteroaggregation observed between Ag, Ti NPs and wastewater components. This study shows that the presence of TiO 2 NPs can affect the stability and toxicity of Ag NPs in complex media that cannot be predicted solely based on ionic, total or nanoparticulate concentrations and the need for studying NP interactions in more complex matrices is highlighted.

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Chronic effects of wastewater-borne silver and titanium dioxide nanoparticles on the rainbow trout (Oncorhynchus mykiss)

Cited by 35 publications

References 67 publications

Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus

Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus

Mild Effects of Sunscreen Agents on a Marine Flatfish: Oxidative Stress, Energetic Profiles, Neurotoxicity and Behaviour in Response to Titanium Dioxide Nanoparticles and Oxybenzone

Wastewater-Aged Silver Nanoparticles in Single and Combined Exposures with Titanium Dioxide Affect the Early Development of the Marine Copepod Tisbe battagliai

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