Chronic effects of tributyltin on multiple biomarkers responses in juvenile common carp, Cyprinus carpio

Abstract

In this study, the chronic toxic effects of tributyltin (TBT), an antifouling paints commonly present in surface and ground water, on morphological indices, reactive oxygen species (ROS) generation, and ATPase activity and heat shock protein (Hsp) 70 protein in tissues (liver, gill, and white muscle) of common carp were investigated. Fish were exposed at sublethal concentrations of TBT (75 ng/L, 0.75 μg/L, and 7.5 μg/L) for 60 days. When compared with the control, there was significant lower condition factor in fish exposed at the higher concentration of TBT. ROS levels in three tissues increased significantly at higher TBT concentrations (0.75 and 7.5 μg/L). The hepatic antioxidant enzymes (total antioxidative capacity and superoxide dismutase) activities were induced at higher concentrations (0.75 μg/L) of TBT. When compared with the hepatic antioxidant enzymes activities in fish exposed to 0.75 μg/L of TBT, there was a decreasing trend in those exposed to TBT with a concentration of 7.5 μg/L. However, all the antioxidant enzymes activities were significantly inhibited in gill and muscle of fish exposed to higher TBT concentrations (0.75 and 7.5 μg/L). Moreover, there was significant lower Na-K-ATPase in three tissues after long-term exposure to higher concentration of TBT, but a significant higher Hsp70 protein levels was observed. In short, environmental concentrations of TBT could not induce obvious impacts on fish, but long-term exposure to higher concentrations of TBT could affect seriously the health status of fish. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 937-944, 2016.

Tributyltin and Zebrafish: Swimming in Dangerous Water

“…Thus, TBT is an inducer of oxidative stress and plays an important role in the positive modulation of pro-inflammatory cytokines ( 45 ). This is consistent with data showing a decreased activity of SOD, CAT, and GPX in other species ( 46 , 47 ), a higher expression of HSP70 in common carp ( 48 ), an increased IL-1β secretion by human immune cells ( 49 ), an increased IL-6 production in human peripheral blood mononuclear cells ( 50 ), and higher TNF-α levels in mouse serum ( 51 ).…”

Histological study of the effect of tributyltin on the adrenal cortical cells of adult male albino rats.

“…A study showed that the toxicity of TBT was less evident in a shorter time of exposure, but the toxicity was increased on longer durations signifying that the effect is time-dependent [62] . This was in line with the current study.…”

Tributyltin-mediated hepatic, renal and testicular tissue damage in male Syrian hamster (Mesocricetus auratus): a study on impact of oxidative stress

“…The enzymatic and non-enzymatic antioxidant defense systems that include SOD, CAT, GPX, vitamin C, vitamin E and GSH become deficient on TBT-exposure. The consequent uncontrolled generation of ROS during TBT-induced toxicity culminates in tissue damage, leading to impaired cellular function, alterations in the physiochemical properties of cellular membranes and reduced enzyme activities (Li et al 2016; Huang et al 2005a, b). …”