Chronic constipation: no longer stuck! characterization of colonic dysmotility as a new disorder in children

Hutson, John M.; Catto-Smith, Tony; Gibb, Susan; Chase, Janet; Shin, Yeong-Myung; Stanton, Michael; King, Sebastian K.; Sutcliffe, Jonathan; Ong, Sim Yee; Djaja, Sebastian; Farmer, Pamela J.

doi:10.1016/j.jpedsurg.2004.02.003

Cited by 29 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). One child (6 years old) with chronic treatment-resistant constipation had seromuscular biopsies collected laparoscopically (King et al 2005) as part of an investigation for changes in SP-, VIP-and NOS-immunoreactive fibres (Hutson et al 2004). This sample was found to have normal levels of these nerve fibres.…”

Section: Patients and Samplesmentioning

confidence: 99%

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system

et al. 2010

Self Cite

View full text Add to dashboard Cite

Cholinergic nerves are identified by labelling molecules in the ACh synthesis, release and destruction pathway. Recently, antibodies against another molecule in this pathway have been developed. Choline reuptake at the synapse occurs via the high-affinity choline transporter (CHT1). CHT1 immunoreactivity is present in cholinergic nerve fibres containing vesicular acetylcholine transporter (VAChT) in the human and rat central nervous system and rat enteric nervous system. We have examined whether CHT1 immunoreactivity is present in nerve fibres in human intestine and whether it is colocalised with markers of cholinergic, tachykinergic or nitrergic circuitry. Human ileum and colon were fixed, sectioned and processed for fluorescence immunohistochemistry with antibodies against CHT1, class III beta-tubulin (TUJ1), synaptophysin, common choline acetyl-transferase (cChAT), VAChT, nitric oxide synthase (NOS), substance P (SP) and vasoactive intestinal peptide (VIP). CHT1 immunoreactivity was present in many nerve fibres in the circular and longitudinal muscle, myenteric and submucosal ganglia, submucosa and mucosa in human colon and ileum and colocalised with immunoreactivity for TUJ1 and synaptophysin confirming its presence in nerve fibres. In nerve fibres in myenteric ganglia and muscle, CHT1 immunoreactivity colocalised with immunoreactivity for VAChT and cChAT. Some colocalisation occurred with SP immunoreactivity, but little with immunoreactivity for VIP or NOS. In the mucosa, CHT1 immunoreactivity colocalised with that for VIP and SP in nerve fibres and was also present in vascular nerve fibres in the submucosa and on epithelial cells on the luminal border of crypts. The colocalisation of CHT1 immunoreactivity with VAChT immunoreactivity in cholinergic enteric nerves in the human bowel thus suggests that CHT1 represents another marker of cholinergic nerves.

show abstract

Section: Patients and Samplesmentioning

confidence: 99%

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…These conditions recognise 2 different pathophysiologic moments as previously demonstrated in adults [4,5]. STC represents a more generalised disturbance of colonic motility in which a reduction of the interstitial cells of Cajal in the myenteric plexus and at the submucosal border has been demonstrated [10] together with a reduction in neurotransmitters as substance P or vasoactive intestinal peptide [9]. FRSO represents a more localised alteration of the rectosigmoid area.…”

Section: Discussionmentioning

confidence: 85%

Scintigraphic evaluation of colonic motility in patients with anorectal malformations and constipation

Zuccarello

Romeo

Scalfari

et al. 2006

Journal of Pediatric Surgery

View full text Add to dashboard Cite

“…Low levels of SP have, however, been reported in both adults [16] and children [21,22,26,27] with STC, suggesting that low SP may be associated with reduced colonic activity. It is not yet known if low SP is a primary anomaly or secondary to chronic constipation.…”

Section: Discussionmentioning

confidence: 99%

An association of multiple endocrine neoplasia 2B, a RET mutation; constipation; and low substance P–nerve fiber density in colonic circular muscle

King

Southwell

Hutson

2006

Journal of Pediatric Surgery

Self Cite

View full text Add to dashboard Cite

Chronic constipation: no longer stuck! characterization of colonic dysmotility as a new disorder in children

Cited by 29 publications

References 41 publications

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system

Scintigraphic evaluation of colonic motility in patients with anorectal malformations and constipation

An association of multiple endocrine neoplasia 2B, a RET mutation; constipation; and low substance P–nerve fiber density in colonic circular muscle

Contact Info

Product

Resources

About