Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides)

Zhao, Liulan; Tang, Gang; Xiong, Chen; Han, Songling; Yang, Chunping; He, Kuo; Li, Qiao; Luo, Jie; Luo, Wei; Wu, Yan; Li, Zhiqiong

doi:10.1016/j.envpol.2021.117010

Cited by 50 publications

(16 citation statements)

References 74 publications

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“…Each sample was run in duplicate, and the relative expressions were calculated according to Vandesompele et al (36). The primers of qPCR were designed using the National Center for Biotechnology Information (NCBI) primer BLAST service or from the previous studies (37)(38)(39)(40)(41)(42) and shown in Table 2.…”

Section: Sample Collection and Growth Performance Determinationmentioning

confidence: 99%

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Gong

Liu

et al. 2022

Front. Nutr.

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High carbohydrate diet (HCD) causes metabolism disorder and intestinal damages in aquaculture fish. Berberine has been applied to improve obesity, diabetes and NAFLD. However, whether berberine contributes to the alleviation of HCD-induced intestinal damages in aquaculture fish is still unclear. Here we investigated the effects and mechanism of berberine on HCD-induced intestinal damages in largemouth bass (Micropterus salmoides). We found dietary berberine (50 mg/kg) improved the physical indexes (VSI and HSI) without affecting the growth performance and survival rate of largemouth bass. Importantly, the results showed that dietary berberine reduced the HCD-induced tissue damages and repaired the barrier in the intestine of largemouth bass. We observed dietary berberine significantly suppressed HCD-induced intestinal apoptosis rate (from 31.21 to 8.35%) and the activity level of Caspase3/9 (P < 0.05) by alleviating the inflammation (il1β, il8, tgfβ, and IL-6, P < 0.05) and ER stress (atf6, xbp1, perk, eif2α, chopa, chopb, and BIP, P < 0.05) in largemouth bass. Further results showed that dietary berberine declined the HCD-induced excessive lipogenesis (oil red O area, TG content, acaca, fasn, scd, pparγ, and srebp1, P < 0.05) and promoted the lipolysis (hsl, lpl, cpt1a, and cpt2, P < 0.05) via activating adenosine monophosphate-activated protein kinase (AMPK, P < 0.05) and inhibiting sterol regulatory element-binding protein 1 (SREBP1, P < 0.05) in the intestine of largemouth bass. Besides, we also found that dietary berberine significantly promoted the hepatic lipid catabolism (hsl, lpl, cpt1a, and cpt2, P < 0.05) and glycolysis (pk and ira, P < 0.05) to reduce the systematic lipid deposition in largemouth bass fed with HCD. Therefore, we elucidated that 50 mg/kg dietary berberine alleviated HCD-induced intestinal damages and improved AMPK/SREBP1-mediated lipid metabolism in largemouth bass, and evaluated the feasibility for berberine as an aquafeed additive to enhance the intestinal function of aquaculture species.

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Section: Sample Collection and Growth Performance Determinationmentioning

confidence: 99%

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Gong

Liu

et al. 2022

Front. Nutr.

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“…Yonar et al (2019) showed that the addition of curcumin in the feed could improve the growth, immunity, and antioxidant capacity of Oncorhynchus mykiss (Yonar et al, 2019). Zhao et al (2021b) reported that curcumin could reduce chlorpyrifos-induced apoptosis and liver damage in largemouth bass (Zhao et al, 2021b). However, curcumin has limited clinical potential, due to its extremely low oral bioavailability due to its poor aqueous solubility and its chemical instability, having an oral bioavailability of only 1% in rats (Yang et al, 2007;Hewlings and Kalman, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Nano-Curcumin Supplementation on Growth Performance, Glucose Metabolism, and Endoplasmic Reticulum Stress in Juvenile Largemouth Bass, Micropterus salmoides

et al. 2022

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This study evaluated the effects of nano-curcumin on growth performance, mucosal immunity, antioxidant response, glucose metabolism, and endoplasmic reticulum stress in largemouth bass. Three groups (three replicates/group) of 270 fish with the mean body weight of 7.00 ± 0.02 g were fed with diets containing 0% (control), 0.1%, and 0.2% nano-curcumin for 60 days. The results showed that dietary supplementation of nano-curcumin had no significant effects on the growth performance, body composition, lysozyme (LYZ), and alkaline phosphatase (AKP) in skin mucus, superoxide dismutase (SOD), and catalase (CAT) activity in serum of largemouth bass. However, dietary supplementation with 0.2% nano-curcumin significantly increased peroxidase (POD) activity in skin mucus and decreased the serum MDA activity compared with the control group. Moreover, dietary supplementation of 0.2% nano-curcumin significantly decreased the levels of serum glucose and liver glycogen, which may be mainly related to the increased gene expression of glucose transporter 2 (GLUT2), glucokinase (GK), phosphofructokinase (PFK), and pyruvate kinase (PK). At the same time, the hepatic gene expression of glucose-regulated protein78 (GRP78), activating transcription factor6 (ATF6), inositol-requiring enzyme 1 (IRE), and eukaryotic initiation factor 2α (eIF2α) in the 0.2% nano-curcumin group were rapidly suppressed, indicating that adding 0.2% nano-curcumin to the diet can alleviate endoplasmic reticulum stress (ERS) in fish. In conclusion, diets supplemented with 0.2% nano-curcumin effectively improved mucosal immunity, antioxidant properties, and glucose metabolism and alleviated ERS induced by long-term carbohydrate intake in largemouth bass.

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“…Results show the upregulation of reactive oxygen species (ROS), and reactive nitrogen species (RNS) promotes cellular alterations and oxidative/nitrative damage and plays a critical role to induce renal apoptosis in the kidneys of goldfish cells, CAS-3 and -9 mRNA levels, and MDA contact in gill tissues (Jiao et al, 2019). Similarly, chlorpyrifos exposure (3 μg/L for 60 days) upregulated the expression of pro-inflammatory genes such as TNF-α, ILα, IL-8, IL-15, downregulated anti-inflammatory genes TGF-ß1, IL-10, and promoted apoptosis through overexpression of CAS-3, À8, À9, and Bax mRNA levels, as well as triggered induction of MDA content in the gill, gut, and liver of largemouth bass, Micropterus salmoides (Zhao et al, 2021). In tilapia, acute exposure to diazinon (0.97 mg/L for 24 h), an organophosphate, caused a significant increase in apoptotic cells ($4-fold) in spleen tissues (Díaz-Resendiz et al, 2019).…”

Section: Interactive Effects Of Heat and Pesticides Exposure On Cellu...mentioning

confidence: 99%

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

Lacy

Rahman

2022

J of Applied Toxicology

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One of many noteworthy consequences of increasing societal reliance on pesticides is their predominance in aquatic environments. These pernicious chemicals interact with high temperatures from global climate change, heat waves, and natural variations to create unstable environments that negatively impact organisms' health.To understand these conditions, we examined the dose-dependent effects of environmentally relevant pesticide mixtures (metolachlor, linuron, isoproturon, tebuconazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) combined with elevated temperatures (22 control vs. 32 C for 4-week exposure) on renin, dinitrophenyl protein (DNP, an indicator of reactive oxygen species, ROS), 3-nitrotyrosine protein (NTP, an indicator of reactive nitrogen species, RNS), superoxidase dismutase (SOD, an antioxidant), and catalase (CAT, an antioxidant) expressions in the kidneys of goldfish (Carassius auratus). Histopathological analysis showed widespread damage to kidney tissues in high temperature and pesticide co-exposure groups, including rupture of the epithelial layer, hemorrhaging, and degeneration of tubular epithelium. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical analyses demonstrated significant declines in renin receptorlike mRNA and protein expressions in kidney tissues under combined exposure to high temperature and pesticides compared with controls; conversely, expression of DNP, NTP, SOD, and CAT increased in kidney tissues under the same conditions. Apoptotic cells were also increased in co-exposure groups as assessed by in situ terminal deoxynucleotidyl transferase dUTP nick labeling (TUNEL) assay. The enhanced apoptosis in kidneys of heat and pesticides co-exposed fish was associated with increased caspase-3 (a protease enzyme) mRNA levels. Our results demonstrated that high temperature and pesticides induced oxidative/nitrative stress (i.e., ROS/RNS), damaged tissues, increased cellular apoptosis, and suppressed renin expression in kidneys of goldfish.

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Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides)

Cited by 50 publications

References 74 publications

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Effects of Dietary Nano-Curcumin Supplementation on Growth Performance, Glucose Metabolism, and Endoplasmic Reticulum Stress in Juvenile Largemouth Bass, Micropterus salmoides

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

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