Chronic caffeine or theophylline intake during pregnancy inhibits A1 receptor function in the rat brain

León, David; Albasanz, José Luís; Ruiz, María Ángeles; Martı́n, Mairena

doi:10.1016/j.neuroscience.2004.11.028

Cited by 29 publications

(35 citation statements)

References 49 publications

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“…Gressens et al (2001) have documented caffeine-induced acceleration of the evagination of neuroepithelium into telencephalic vesicles (Sahir et al, 2000) and upregulation of sonic hedgehog (Sahir et al, 2004). Gestational caffeine also downregulates central adenosine receptors (Leon et al, 2005;Lorenzo et al, 2010), reduces NMDA antagonist-induced locomotor activity (da Silva et al, 2005), and affects respiratory control (Bodineau et al, 2003;Saadani-Makki et al, 2004). Cognitive and motor deficits have also been reported in adult rodents exposed in caffeine during gestation and/or early postnatal life (Bjorklund et al, 2008;Soellner et al, 2009), although some impairments may be paradigm specific (Soellner et al, 2009).…”

Section: Caffeinementioning

confidence: 99%

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Ross

Graham

Money

et al. 2014

Neuropsychopharmacol

333

241

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Most drugs of abuse easily cross the placenta and can affect fetal brain development. In utero exposures to drugs thus can have long-lasting implications for brain structure and function. These effects on the developing nervous system, before homeostatic regulatory mechanisms are properly calibrated, often differ from their effects on mature systems. In this review, we describe current knowledge on how alcohol, nicotine, cocaine, amphetamine, Ecstasy, and opiates (among other drugs) produce alterations in neurodevelopmental trajectory. We focus both on animal models and available clinical and imaging data from cross-sectional and longitudinal human studies. Early studies of fetal exposures focused on classic teratological methods that are insufficient for revealing more subtle effects that are nevertheless very behaviorally relevant. Modern mechanistic approaches have informed us greatly as to how to potentially ameliorate the induced deficits in brain formation and function, but conclude that better delineation of sensitive periods, dose-response relationships, and long-term longitudinal studies assessing future risk of offspring to exhibit learning disabilities, mental health disorders, and limited neural adaptations are crucial to limit the societal impact of these exposures.

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Section: Caffeinementioning

confidence: 99%

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Ross

Graham

Money

et al. 2014

Neuropsychopharmacol

333

241

View full text Add to dashboard Cite

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“…Adenosine receptors regulate glucose metabolism in insulin sensitive tissues such as muscle (Vergauwen et al, 1994), and caffeine is well known as a non-specific adenosine receptor antagonist. It is reported that caffeine affects A1 receptors, a member of the adenosine receptor group, and reduces insulin sensitivity in vivo (Vergauwen et al, 1994;León et al, 2005;Johansson et al, 2007) and in vitro (Akiba et al, 2004), and that caffeine ingestion negatively affects insulin sensitivity during oral glucose tolerance test in type 2 diabetes subjects (Robinson et al, 2004). BT1 contains abundant gallate-type catechins including EGCg, and their content in BT1 is 2.35-fold of BT2.…”

Section: Discussionmentioning

confidence: 99%

Concentration of Catechins and Caffeine in Black Tea Affects Suppression of Fat Accumulation and Hyperglycemia in High-fat Diet-fed Mice

Imada

Tanaka

Nishiumi

et al. 2011

FSTR

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In this study, we investigated preventive effects of black tea on obesity and hyperglycemia in C57BL/6 mice fed a high-fat diet for 14 weeks. During the feeding period, mice were given the following black teas: BT1 containing 3,000 mg/L total catechins and 864 mg/L caffeine; and BT2 containing 1,437 mg/L total catechins and 594 mg/L caffeine. Both BT1 and BT2 suppressed high-fat diet-caused body weight gain, deposition of white adipose tissue, and increases in plasma lipids and glucose. Moreover, both BT1 and BT2 counteracted the high-fat diet-caused decrease in the expression of insulin receptor (IR)-β and glucose transporter 4 (GLUT4), markers for insulin resistance. Of these effects, BT2 were stronger than that of BT1. In conclusion, black tea prevents hyperglycemia and insulin resistance through maintenance of IR-β and GLUT4 expression in high-fat diet-fed mice, with an appropriate concentration of tea producing maximal effectiveness.

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“…18 The effect of caffeine during gestation was extended to the transduction pathway, including G-protein and adenylyl cyclase activity mediated by these receptors, causing a loss of system responsiveness only in the maternal brain, but downregulating G i1 protein in both the maternal and fetal brains. 19 In addition, A 1 R was also significantly decreased in the brain from mothers treated with caffeine only during lactation or throughout gestation and the lactation period. Interestingly, this receptor also diminished in the brain from 15-day-old neonates in a gender-dependent way, while A 2A R remained unaltered.…”

Section: Introductionmentioning

confidence: 92%

“…Western blotting was performed as described earlier. 19 Immunodetection was carried out by incubating the nitrocellulose membranes with specific polyclonal antibody (anti-A 1 , anti-aG 1-2 , and anti-aG 3 ). After washing, blots were incubated with horseradish peroxidasecoupled goat anti-rabbit or mouse IgG diluted 1:3,000.…”

Section: Rat Heart Membranes Isolationmentioning

confidence: 99%

“…17,19 Primers used were: forward 5¢-ATC CCA CTG GCC ATC CTT ATG-3¢ and reverse 5¢-TGG CGA TGT AGA TCA GAA TGC-3¢ for A 1 R; 5¢-CCA TGC TGG GCT GGA ACA-3¢ and 5¢-GAA GGG GCA GTA ACA CGA ACG-3¢ for A 2A R; 5¢-AAG GAC AGC GTG TGC AAG CCT GCT TCA AC-3¢ and 5¢-AAT CTG TCA TTC CGT ACA AGG TAC TTA ACA-3¢ for aGi 1 , and 5¢-GCA GGC TGC AAG GAG CAA CAG CGA-3¢ and 5¢-CTC CTG GGT GCG CAC TGG GCC-3¢ for aGs. PCR products were analyzed by electrophoresis in 2% agarose gels and stained with ethidium bromide.…”

Section: Reverse Transcription Polymerase Chain Reaction Analysismentioning

confidence: 99%

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Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A₁ and A_2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats

Iglesias

Albasanz

Martı́n

2014

Journal of Caffeine Research

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Background: Caffeine is the most widely consumed psychoactive substance in the world, even during pregnancy. Its stimulatory effects are mainly due to antagonism of adenosine actions by blocking adenosine A 1 and A 2A receptors. Previous studies have shown that caffeine can cross the placenta and therefore modulate these receptors not only in the fetal brain but also in the heart. Methods: In the present work, the effect of caffeine chronically consumed during pregnancy on A 1 and A 2A receptors in Wistar rat heart, from both mothers and their fetuses, were studied using radioligand binding, Western-blotting, and adenylyl cyclase activity assays, as well as reverse transcription polymerase chain reaction. Results: Caffeine did not significantly alter A 1 R neither at protein nor at gene expression level in both the maternal and fetal heart. On the contrary, A 2A R significantly decreased in the maternal heart, although mRNA was not affected. Gi and Gs proteins were also preserved. Finally, A 1 R-mediated inhibition of adenylyl cyclase activity did not change in the maternal heart, but A 2A R mediated stimulation of this enzymatic activity significantly decreased according to the detected loss of this receptor. Conclusions: Opposite to the downregulation and desensitization of the A 1 R/AC pathway previously reported in the brain, these results show that this pathway is not affected in rat heart after caffeine exposure during pregnancy. In addition, A 2A R is downregulated and desensitized in the maternal heart, suggesting a differential modulation of these receptor-mediated pathways by caffeine.

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Chronic caffeine or theophylline intake during pregnancy inhibits A1 receptor function in the rat brain

Cited by 29 publications

References 49 publications

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Concentration of Catechins and Caffeine in Black Tea Affects Suppression of Fat Accumulation and Hyperglycemia in High-fat Diet-fed Mice

Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A₁ and A_2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats

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Chronic caffeine or theophylline intake during pregnancy inhibits A1 receptor function in the rat brain

Cited by 29 publications

References 49 publications

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Developmental Consequences of Fetal Exposure to Drugs: What We Know and What We Still Must Learn

Concentration of Catechins and Caffeine in Black Tea Affects Suppression of Fat Accumulation and Hyperglycemia in High-fat Diet-fed Mice

Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A1 and A2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats

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Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A₁ and A_2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats