Chronic adolescent morphine exposure alters the responses of lateral paragigantocellular neurons to acute morphine administration in adulthood

Salmanzadeh, Hamed; Azizi, Hossein; Ahmadi-Soleimani, S. Mohammad; Pachenari, Narges; Semnanian, Saeed

doi:10.1016/j.brainresbull.2017.12.007

Cited by 19 publications

(16 citation statements)

References 37 publications

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“…Our previous findings revealed that by administration of escalating doses of morphine between PNDs 31 and 40 in male rats, the development of morphine tolerance and morphine dependency increases in the adulthood (Salmanzadeh, Azizi, & Semnanian, ). Furthermore, our findings demonstrated that the baseline activity of LPGi neurons, a nucleus which is involved in mediating opiate effects in the brain (Ahmadi‐Soleimani, Azizi, Gompf, & Semnanian, ; Ahmadi‐Soleimani, Ghaemi‐Jandabi, Azizi, & Semnanian, ; Ghaemi‐Jandabi, Azizi, & Semnanian, ; Kaeidi et al, ), was enhanced in adolescent morphine‐treated animals during adulthood (Salmanzadeh, Azizi, Soleimani, Pachenari, & Semnanian, ).…”

Section: Introductionmentioning

confidence: 55%

Adolescent morphine exposure increases nociceptive behaviors in rat model of formalin test

Ghasemi

Pachenari

Semnanian

et al. 2018

Developmental Psychobiology

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The number of adolescents who use illicit drugs has increased dramatically. Adolescence is a critical period for brain development and maturation. The importance of the study of pain perception and the possible mechanisms involved is crystal clear. Up until now, there has been no evidence regarding the long‐term effect of adolescence morphine administration on pain perception. The objective of the present study was to investigate long‐lasting effect of adolescent morphine exposure on pain perception as well as analgesic response to a single dose of morphine injection. Adolescent and adult rats received morphine or saline, and then after 30 days of washout period, formalin test was performed. To evaluate morphine analgesia, in a separate group of animals, formalin test was performed after injection of a single dose of morphine during adulthood. The results demonstrated that the adolescent rats treated by morphine exhibited higher pain‐related behaviors compared to the control group, while the same results were not observed in adult rats that had been treated by morphine. Moreover, there was no significant difference in analgesic response to a single dose of morphine between two experimental groups. This study demonstrates enduring effect of morphine exposure during adolescence on pain perception.

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Section: Introductionmentioning

confidence: 55%

Adolescent morphine exposure increases nociceptive behaviors in rat model of formalin test

Ghasemi

Pachenari

Semnanian

et al. 2018

Developmental Psychobiology

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“…All injections were subcutaneous and were administered twice daily (8:00 a.m. and 5:00 p.m.). The morphine group in accordance with past studies received increasing morphine doses (2.5, 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25 mg/kg) (Khani et al., 2022; Salmanzadeh et al., 2017; Salmanzadeh et al., 2018). After a 7‐days drug‐free period, the open field test (OFT) for detecting locomotor activity (PND 48, PND 56, PND 64, and PND 72) and elevated plus maze (EPM) test to identify anxiety‐like behavior (PND 49, PND 57, PND 65, and PND 73) were performed over a 4‐week postmorphine period from adolescence to adulthood.…”

Section: Methodsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 98%

Development of anxiety‐like behaviors during adolescence: Persistent effects of adolescent morphine exposure in male rats

Khani

Pourmotabbed

Hosseinmardi

et al. 2022

Developmental Psychobiology

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Epidemiological studies show the prevalence of opioid use, misuse and abuse in adolescents, which imposes social and economic accountability worldwide. Chronic opioid exposure, especially in adolescents, may have lasting effects on emotional behaviors that persist into adulthood. The current experiments were therefore designed to study the effects of sustained opioid exposure during adolescence on anxiety-like behaviors. Adolescent male Wistar rats underwent increasing doses of morphine for 10 days (PNDs 31-40). After that the open field test (OFT) and elevated plus maze (EPM) test were performed over a 4-week postmorphine treatment from adolescence to adulthood. Moreover, the weight of the animals was measured at these time points. We found that chronic adolescent morphine exposure reduces the weight gain during the period of morphine treatment and 4 weeks after that. It had no significant effect on the locomotor activity in the animals. Moreover, anxiolytic-like behavior was observed in the rats exposed to morphine during adolescence evaluated by OFT and EPM test.Thus, long-term exposure to morphine during adolescence has the profound potential of altering the anxiety-like behavior profile in the period from adolescence to adulthood. The maturation of the nervous system can be affected by drug abuse during the developmental window of adolescence and these effects may lead to behaviorally stable alterations.

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“…LPGi recording. As previously described (Salmanzadeh et al, 2018;Torabi et al, 2019), the spontaneous activity of LPGi neurons was recorded by glass micropipette (OD: 1.2 mm, ID: 0.94 mm; Sutter Instruments, Novato, CA) filled with 2% pontamine sky blue, which was dissolved in 0.5 M sodium acetate (3-12 MΩ resistance). The observation of a burst-like neuronal discharge (as a typical characteristic of Bötzinger or pre-Bötzinger nuclei) immediately above the LPGi area was considered as an electrophysiological landmark.…”

Section: Extracellular Single-unit Recordingmentioning

confidence: 99%

“…It also has been reported that lesioning the PGi reduces opioid withdrawal-induced hyperactivity of LC neurons (Rasmussen and Aghajanian, 1989). Further studies conducted in our lab revealed that adolescent morphine exposure alters the electrical activity of LPGi neurons in response to a morphine challenge during adulthood (Salmanzadeh et al, 2018, 2020). Here, we first investigate whether patrilineal exposure to morphine during adolescence can alter the opiate withdrawal-induced behavioral signs in male offspring.…”

Section: Introductionmentioning

confidence: 97%

Paternal exposure to morphine during adolescence potentiates morphine withdrawal in male offspring: Involvement of the lateral paragigantocellularis nucleus

2020

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Background: Opiate exposure during adolescence perturbs the brain’s maturation process and potentially confers long-term adverse consequences, not only in exposed individuals but also in their posterity. Here, we investigate the outcomes of adolescent paternal morphine exposure on morphine withdrawal profile in male offspring. Methods: Male Wistar rats were chronically subjected to 10 days of an escalating regimen of morphine during adolescence. After a 20-day washout period, adult males were allowed to copulate with naïve females. The adult male offspring were tested for somatic and affective components of naloxone-precipitated morphine withdrawal using conditioned place aversion. Moreover, electrical activity of the lateral paragigantocellularis (LPGi) nucleus, which is involved in development of opiate dependence, was recorded in response to a challenge dose of morphine via extracellular single-unit recordings. Results: Morphine-sired offspring exhibited augmented expression of naloxone-induced somatic and affective signs of opiate withdrawal compared to the control saline-sired counterparts. In vivo recording revealed that LPGi neurons displayed heterogeneous responses (inhibitory, excitatory, and no change) to acute morphine administration in both morphine- and saline-sired animals. The morphine-induced discharge inhibition was potentiated in morphine-sired offspring. However, the extent of discharge excitation in response to morphine did not reach significance in these subjects. Moreover, the lack of alteration in maternal behavior toward morphine-sired offspring indicates that this is due to germline-dependent transmission of epigenetic traits across generations. Conclusions: Preconception paternal exposure to morphine during adolescence potentiates opiate withdrawal signs in male offspring which is mediated, at least in part, by epigenetic alteration of LPGi-related brain circuitry.

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Chronic adolescent morphine exposure alters the responses of lateral paragigantocellular neurons to acute morphine administration in adulthood

Cited by 19 publications

References 37 publications

Adolescent morphine exposure increases nociceptive behaviors in rat model of formalin test

Adolescent morphine exposure increases nociceptive behaviors in rat model of formalin test

Development of anxiety‐like behaviors during adolescence: Persistent effects of adolescent morphine exposure in male rats

Paternal exposure to morphine during adolescence potentiates morphine withdrawal in male offspring: Involvement of the lateral paragigantocellularis nucleus

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