Pteris laeta Wall., as a traditional
tea, is popular in Southwest China, but its role in preventing cognitive
impairment is unclear. In this study, Pteris laeta Wall. extracts (PW) and its active compounds were evaluated for
preventive effects on Alzheimer’s disease (AD) in vivo and in vitro. The results showed that PW diminished
oxidative stress damage and apoptosis of Aβ-induced HT22 cells
and also rescued cognitive deficits, and ameliorated pathological
injury and inflammatory response in APP/PS1 mice. Besides, a new pterosin
sesquiterpene, named pterosinsade A (PA), and nine known compounds
were discovered from the EtOAc extract that possessed the best neuroprotective
activity. PA reduced apoptosis of APP-overexpressing neural stem cells
and promoted their proliferation and neuronal differentiation. Meanwhile,
PW and PA promoted hippocampal neurogenesis, which proved to be associated
with activating the Wnt signaling pathway. These findings suggest
that PW and PA are candidates for AD prevention.