Chromosomes of Mouse Embryos and Newborn Young: Preparations from Membranes and Tail Tips

Evans, E. P.; Burtenshaw, M. D.; Ford, C. E.

doi:10.3109/10520297209116541

Cited by 125 publications

(38 citation statements)

References 12 publications

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“…After the trypsin was inactivated in culture medium and the cells were washed, the dissociated cells were incubated at 37°C in 0.1 M KCl hypotonic solution for ∼25 min. The cells then were fixed in 3:1 methanol:acetic acid mixture on ice and spread on slides for metaphase preparations according to described protocols (80). The slides were dried overnight and stained with DAPI, and the chromosomes were imaged using a 63× oil objective on a Leica SP5 confocal microscope (Leica).…”

Section: Methodsmentioning

confidence: 99%

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Bazzi

Anderson

2014

Proc. Natl. Acad. Sci. U.S.A.

173

201

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Significance Centrioles form the core of centrosomes, which organize cilia and interphase and spindle microtubules in animal cells, but centrosome function has not been defined in mammals in vivo. We show that mouse embryos that lack centrioles and centrosomes survive to midgestation, when they lack primary cilia and cilia-dependent signaling. Despite the absence of centrosomes, bipolar spindle formation, chromosome segregation, cell-cycle profile, and DNA damage response are normal in the mutants. Unlike mutants that lack cilia, most cells in acentriolar embryos activate a p53-dependent apoptotic pathway. The data show that mammalian centrioles promote the efficient and rapid assembly of the mitotic spindle and that a short delay in prometaphase activates a checkpoint that leads to p53-dependent cell death in vivo.

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Section: Methodsmentioning

confidence: 99%

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Bazzi

Anderson

2014

Proc. Natl. Acad. Sci. U.S.A.

173

201

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“…A small piece of liver, removed without ligature, was placed in Ham's FlO medium containing 10% foetal calf serum, antibiotics and colcemid (0·05 pg/ml) and incubated at 35°C for 2h before harvesting for chromosome preparations (Evans et al 1972). The incisions were then closed and the animal injected with colchicine (0·33 pg/g).…”

Section: Methodsmentioning

confidence: 99%

“…Ages of pouch young were estimated from the data of Lyne (1964) for P. nasuta and Mackerras and Smith (1960) for I. macrourus. Chromosomes were prepared according to the techniques of Ford et al (1958) and Evans et al (1972). Except where shown, at least 20 cells per tissue were counted from each animal.…”

Section: Methodsmentioning

confidence: 99%

Sex Chromosome Mosaicism in Liver, Thymus, Spleen and Regenerating Liver of Perameles Nasuta and Isoodon Macrourus

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1979

Aust. Jnl. Of Bio. Sci.

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An X chromosome disappears from female cells and the Y chromosome disappears from male cells during development in some somatic tissues of the bandicoots P. nasuta and l. macrourus leaving the cells with a 2n = 13 (Le. XO) chromosome complement. In order to determine time of disappearance of the relevant sex chromosome, counts were made from dividing cells in liver, thymus and spleen of both species at various stages of development. Histological studies of liver and thymus were made at the same developmental stages. Frequencies of 2n = 14 cells (Le. those containing both sex chromosomes) were high in the liver and thymus of animals 1-4 days old but were low at 15 days of age when haematopoietic cells predominated. While most cells of thymus and spleen remained 2n = 13, the frequency of 2n = 14 cells rose again in the liver of animals aged over 20 days at which time blood-forming activity was considerably diminished. It is suggested that blood-forming cells of both species of bandicoot discard a sex chromosome at an early stage of differentiation.As mitoses were rare in livers of older animals partial hepatectomies were performed on juveniles and adults of both species in order to stimulate cell division. Mter 72 h of regeneration 28 of 36 dividing cells were 2n = 13 whereas one of 55 cells was 2n = 13 after 77 h.

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“…The latter technique is notable for the use of Concanavalin A as a mitogen, and the higher dose of Glutamine. Liver from newborn rats and spleen from adult rats could be cultured to make chromosome preparations within two hours of sacrificing the animal with modification of the methods of Evans et al, (1972) and Stock et al, (1972).…”

Section: Methodsmentioning

confidence: 99%

B chromosomes in Rattus fuscipes I. Mitotic and meiotic chromosomes and the effects of B chromosomes on chiasma frequency

1984

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Chromosomes of Mouse Embryos and Newborn Young: Preparations from Membranes and Tail Tips

Cited by 125 publications

References 12 publications

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Sex Chromosome Mosaicism in Liver, Thymus, Spleen and Regenerating Liver of Perameles Nasuta and Isoodon Macrourus

B chromosomes in Rattus fuscipes I. Mitotic and meiotic chromosomes and the effects of B chromosomes on chiasma frequency

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