Chromosomes in Hodgkin's disease and other malignant lymphomas

Abstract

Direct chromosome preparations have been made on biopsies from 12 untreated cases of malignant lymphoma. In a majority of cases there appears to be accentuation of secondary constriction in chromosome 9 and occasionally in other chromosomes. In aneuploid cells gains or losses for particular homologues may be nonrandom. Ten of the 12 cases showed some apparently classically diploid cells. In 5 of 6 cases of Hodgkin's disease (H.D.) some hyperdiploid, usually near‐tetraploid, cells were found and are presumed to represent Reed‐Sternberg cells. In one case of H.D. quasi‐diploid cells predominated. In one case of H.D. and in one case of lymphosarcoma only apparently classically diploid cells were found. Four reticulum cell sarcomas had abnormal karyotypes with stemlines varying from near‐diploid to hypertetraploid.

“sternberg‐reed” giant cells of hodgkin's disease: Cultivation in vitro, heterotransplantation, and characterization as neoplastic macrophages

“…The soundest evidence that Hodgkin's disease is indeed a malignant neoplastic condition rests on the demonstration that fresh biopsy material and shortterm cultures from such biopsies contain aneuploid, often hypotetraploid cells (Miles et al, 1966;Seif and Spriggs, 1967;Whitelaw, 1969;Coutinho et a1.,1971;Demin et al, 1972), and on the further demonstration by Seif and Spriggs (1967) that all of the aneuploid cells in one of their cases contained two very distinctive giant marker chromosomes, and therefore must have been clonally derived. Accordingly, cells cultured from tissues involved by Hodgkin's disease cannot be deemed to have been derived from the neoplastic (Sternberg-Reed and Hodgkin) cell population unless they can be shown to be aneuploid, and/or to satisfy other biological criteria of neoplasia, such as heterotransplantability, after a limited time in culture (Epstein and Kaplan, 1974;Nilsson and Pontkn, 1975;Epstein et al, 19766).…”

“…However, this interpretation has been difficult to reconcile with their sparse numbers in most tissue specimens, and has been further clouded by the results of cytologic and autoradiographic investigations. Two populations of mitotic cells have been observed in fresh biopsy material or short-term culture treated with colchicine or vinblastine (Ricci et a!., 1962; Galan et al, 1963;Raker and Atkin, 1965;Miles et al, 1966;Seif and Spriggs, 1967;Millard, 1968 ;Spiers and Baikie, 1968;Whitelaw, 1969;Coutinho et al, 1971;Demin et al, 1972;Reeves, 1973). One of these has a normal diploid modal chromosome number of 46, and is thought to represent the normal lymphoid cells of the stroma; the second class of mitotic cell, seen less consistently and usually in smaller numbers, is aneuploid, and often hypotetraploid.…”

Chromosomes and Transformation of Lymphocytes in Lymphoproliferative Disorders

“…In the other lymphoproliferative disorders some cells with an abnormal karyotype are almost always found in direct preparations or short-term cultures of the nodes (Baker and Atkin, 1965;Miles et al, 1966;Millard, 1968). These abnormal cells probably represent dividing tumour cells.…”

Clinical and cytogenetic analysis of human anemias from Jammu region of Jammu and Kashmir state

“…Cell suspension was then fixed in 3:1 methanol and acetic acid and the same was subsequently processed for the chromosome study, following Miles et al . [11] with slight modifications. About 7-days old slides were G-banded following Sea bright[12] with slight modifications.…”