Chromosome rearrangements are not accompanied by expected genome size change in the tree weta Hemideina thoracica (Orthoptera, Anostostomatidae)

Morgan‐Richards, Mary

doi:10.1665/1082-6467(2005)14[143:cranab]2.0.co;2

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…Chromosome preparation followed the procedure of Morgan‐Richards () without colchicine pre‐treatment. Although colchicine can improve yields of chromosome spreads, it prevents spindle fibre formation so that the chromosomes do not separate.…”

Section: Methodsmentioning

confidence: 99%

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Multiple lines of evidence suggest mosaic polyploidy in the hybrid parthenogenetic stick insect lineageAcanthoxyla

Myers

Trewick

Morgan‐Richards

2012

Insect Conserv Diversity

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Although hybridisation is common in animals, it rarely results in speciation. Yet, many examples of hybrid species have been documented in one animal group, the stick insects (Phasmida). The New Zealand stick insect Acanthoxyla is of particular interest as the entire genus is of hybrid origin and consists of eight morphological forms recognised as species, all of which are obligate parthenogens. Using five complementary techniques on the same individuals, our study confirms that both triploids and diploids are present in Acanthoxyla populations, and further, that some individuals contain both diploid and triploid cells. Chromosome spreads and estimates of relative DNA content from flow cytometry provided contrasting information about the ploidy of this unusual parthenogenetic genus. Analysis of morphometric variation showed no correlation with ploidy level in Acanthoxyla, and also mtDNA sequence networks failed to distinguish morphospecies or ploidy level. Unexpectedly, cloned sequences of a putatively single‐copy nuclear marker were also unhelpful in distinguishing ploidy, instead indicating that phosphoglucose isomerase is likely to be a multiple copy gene. We propose a mechanism for the evolution of the Acanthoxyla lineage and suggest that interpretation may be complicated by the presence of individuals that are diploid and triploid mosaics.

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Section: Methodsmentioning

confidence: 99%

“…Our protocol followed that of Galbraith et al . () with modifications (Morgan‐Richards, ). Samples consisting of 10 mm of tibia were cut from each stick insect and chopped in a Petri dish with nuclear isolation buffer.…”

Section: Methodsmentioning

confidence: 99%

Multiple lines of evidence suggest mosaic polyploidy in the hybrid parthenogenetic stick insect lineageAcanthoxyla

Myers

Trewick

Morgan‐Richards

2012

Insect Conserv Diversity

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“…Tree and giant wētā (genera Hemideina and Deinacrida) do appear to have a high tolerance for chromosome rearrangements, as is evident from the large number of distinct karyotypes and hybrid zones within three species. The variation seen in Hemideina appears to be the result of Robertsonian translocations, rather than duplication or loss of genetic material, as Morgan-Richards (2005) showed that genome size was similar among different chromosome races of H. thoracica.…”

Section: Chromosomementioning

confidence: 99%

Comparative cytogenetics of North Island tree wētā in sympatry

Mckean

Trewick

Morgan‐Richards

2015

New Zealand Journal of Zoology

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In North Island New Zealand three species of tree wētā (Hemideina) have narrow regions of overlap. Using detailed measurements of chromosomes we compared the karyotypes of Hemideina thoracica with those of Hemideina crassidens and Hemideina trewicki. Although H. thoracica and H. trewicki have the same diploid number (2n = 17 [XO], 18 [XX]), distinct from H. crassidens (2n = 15 [XO] 16 [XX]); the karyotypes of H. trewicki and H. crassidens are more similar to each other than either is to H. thoracica. Elements within each karyotype were identified that are species specific and will aid identification of putative hybrids. Quantitative cytogenetics was used to identify the sex chromosome for H. crassidens and H. trewicki, which in contrast to previous inferences, is most likely the fifthlongest metacentric chromosome in H. crassidens, and the third-longest metacentric chromosome in H. trewicki.

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Chromosome rearrangements are not accompanied by expected genome size change in the tree weta Hemideina thoracica (Orthoptera, Anostostomatidae)

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 2 publications

References 16 publications

Multiple lines of evidence suggest mosaic polyploidy in the hybrid parthenogenetic stick insect lineageAcanthoxyla

Multiple lines of evidence suggest mosaic polyploidy in the hybrid parthenogenetic stick insect lineageAcanthoxyla

Comparative cytogenetics of North Island tree wētā in sympatry

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