Chromosome Preparatons of Bone Marrow Cells without Prior<i>In Vitro</i>Culture or<i>In Vivo</i>Colchicine Administration

Tjio, J. H.; Whang, John

doi:10.3109/10520296209114563

Cited by 508 publications

(99 citation statements)

References 5 publications

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“…An unusually high percentage of metaphases from several preparations of an iliac bone marrow aspirate [16] were not analyzable. Of the analyzable metaphases, 275 were diploid and 2 were tetraploid.…”

Section: Bone Marrow Prebarationsmentioning

confidence: 99%

Tetraploid-Diploid Mosaicism in a Surviving Infant

et al. 1967

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Although tetraploidy and tetraploid-diploid mosaicism has been well documented in studies of aborted human fetuses, no living case has been reported. This report concerns a male infant with tetraploiddiploid mosaicism who survived for 36 weeks. Because the peripheral blood cells of this infant displayed gross differences both in cell size and in DNA content, it was possible to establish that the tetraploidy observed in cultured cells was a true reflection of in vivo mosaicism.The propositus was born on December 6, 1965, after a 42-week gestation notable for a paucity of fetal movement. Birth weight was 1.7 kg, length 40.6 cm and head circumference 25.4 cm. He presented with multiple congenital anomalies. Peripheral blood smears appeared to contain two populations of neutrophilic granulocytes that differed in overall size.Immunological assessment gave the following results : 1. Serum immunoelectrophoresis performed at the ages of 4, 10 and 14 weeks showed qualitatively normal immunoglobulins. 2. Normal antibody responses were obtained to type I polio and to typhoid-paratyphoid vaccines given at age 10 weeks.3. Normal delayed hypersensitivity was obtained following stimulation with 2,4-dinitrofluorobenzene at the age of 12 weeks. There was a normal plasma growth hormone response to insulin-induced hypoglycemia at 12 weeks of age.Many of the cells in follicular centers of the spleen and lymph nodes were pale, and the lymphocytes surrounding them were bizarre, being unusually large and hyperchromatic. The histologic architecture was preserved and cortico-medullary differentiation maintained.Tetraploid-diploid mosaicism was found in ten short-term cultures of peripheral blood leukocytes obtained over a seven-month period. The average proportion of tetraploid cells was 69 % (range 64 to 77 %).Chromosomal analysis of six different primary fibroblast cultures, including that derived from the postmortem lung, were diploid in number and euploid in karyotype.Peripheral blood smears appeared to have two populations of neutrophilic granulocytes which differed from one another in their overall size. The distributions of cell diameters from all of the subject's smears showed highly significant positive skewing, in contrast to the more symmetrical distributions from the control smears.Analysis of bimodality, using a computer, indicated the presence of two normally distributed subpopulations. The,results generated are given in table IV.Cytophotometric measurements of cellular DNA content can be used to analyze tetraploid-diploid mosaicism, since tetraploid cells have twice as much DNA as diploid cells. The large cells in peripheral blood from the subject had 2.01 times the stain content of his normal appearing cells, and 1.91 times that of the control. I n order to estimate the incidence of tetraploidy among the subject's leukocytes, measurements were made on randomly selected cells a n d gave the results shown i n table V.

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Section: Bone Marrow Prebarationsmentioning

confidence: 99%

Tetraploid-Diploid Mosaicism in a Surviving Infant

et al. 1967

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“…testes were dissected out in normal saline. Testis follicles were treated separately with one of the hypotonic solutions (TJIO and WHANG 1962), like potassium chloride (KCl) and sodium citrate (Na citrate). 0.016 M Na citrate gave the best results in the preparation of mitotic as well as meiotic metaphase chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Achar¹

1986

Caryologia

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“…Testes were dissected out in normal saline and pretreated for about an hour at room temperature with hypotonic solutions (TJIO and WHANG 1962) such as potassium chloride (KCI) (HUNGERFORD 1965) and sodium citrate (Na citrate) at varying molarities. The use of KCl at 0.125 to 0.150M yeilded fairly good results in the preparation of meiotic prophase chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Male Meiosis in Five Species of Indian Diplopoda (Myriapoda)

Achar

1984

Caryologia

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SUMMARY -A sequential study of male meiosis of five species of south Indian Diplopoda (Myriapoda), namely, Cingalobolus sp., Trigoniulus sp., (Family Trigoniulidae), Carlogonus palmatus, Thyropygus sp., and Thyropygus alienus (Family Harpagophoridae) has been made by the use of air drying technique. The pretreatment of mitotic and meiotic tissues with colchicine (0.05%) and hypotonic Na citrate (0.016-0.021M) and KCl (0.125-0.150M) has enabled to secure well-spread mitotic and meiotic chromosomes, which, otherwise were found to be cytologically difficult to obtain. Some significant cytological features, such as heteropycnosis, premetaphase stretching of chromosomes, nonstaining gaps in the chromosomal bivalents and ring formation by the sex-chromosomes (XY) have been discussed.

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Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration

Cited by 508 publications

References 5 publications

Tetraploid-Diploid Mosaicism in a Surviving Infant

Tetraploid-Diploid Mosaicism in a Surviving Infant

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Analysis of Male Meiosis in Five Species of Indian Diplopoda (Myriapoda)

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