Chromosome Pairing: Effect of Colchicine on an Isochromosome

Driscoll, C. J.; Darvey, Norman L.

doi:10.1126/science.169.3942.290

Cited by 83 publications

(46 citation statements)

References 3 publications

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“…Driscoll and Darvey (1970) observed that colchicine affects the spatial relationship of homoeologous chromosomes but not the formation of chiasmata. Additionally, they suggested that chromosome position is crucial in the meiotic pairing of homologous and homoeologous chromosomes, altering the arrangement of chromosomes in the nuclear membrane, and allowing the expression of cryptic genomic homology.…”

Section: Introductionmentioning

confidence: 96%

“…First, the expected alteration of the spatial relation of chromosomes (Driscoll and Darvey 1970) was not observed, hence, they were able to maintain their ordering Avivi 1988, Feldman et al 1997) and anchor to the nuclear membrane (Bass et al 2000, Chikashige et al 2010, favoring pairing among homologous chromosomes. Second, the pam1 gene, which intervenes in the presynaptic mechanisms (Felman andAvivi 1988, Bozza andPawlowski 2008) and in the chromosome reorganization related to the formation of the bouquet (Zickler andKleckner 1998, Bass et al 2000), may have a different effect on Z. diploperennis than on the other Zea species.…”

Section: Cryptic Pairing Among Homoeologous Chromosomes Of the Genus Zeamentioning

confidence: 99%

See 1 more Smart Citation

Cryptic homoeology analysis in species and hybrids of genus Zea

Molina¹,

López²,

Staltari³

et al. 2013

Biol. plant.

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Section: Introductionmentioning

confidence: 96%

Section: Cryptic Pairing Among Homoeologous Chromosomes Of the Genus Zeamentioning

confidence: 99%

Cryptic homoeology analysis in species and hybrids of genus Zea

Molina¹,

López²,

Staltari³

et al. 2013

Biol. plant.

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show abstract

“…Interestingly, the link between cytoskeleton and prophase chromosome dynamics in plants had been identified indirectly even before the development of methods for live imaging of meiotic chromosomes. In the 1970s, Driscoll and Darvey showed that colchicine disrupts homologous chromosome pairing (Driscoll and Darvey, 1970). Furthermore, Cowan and Cande demonstrated that colchicine forestalls the bouquet formation .…”

Section: The Role Of Cytoskeleton In Chromosome Dynamicsmentioning

confidence: 99%

Chromosome Organization and Dynamics during Interphase, Mitosis, and Meiosis in Plants

2011

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“…Even though chromosome movements in maize exhibit similar velocities to chromosome movements in budding yeast, meiotic prophase in maize is several fold-longer (22,29,30), which could be a reflection of the fact that maize chromosomes are much longer than yeast chromosomes and, consequently, require longer time to find their correct pairing partners. Also, colchicine, which stops nuclear motility in maize, has been shown to cause homologous pairing defects in plant meiocytes (31). The nuclear movements during pachynema in maize show different patterns than the zygotene movements, suggesting that they have a different role.…”

Section: Diversity Of Meiotic Chromosome Dynamics Patterns Among Specmentioning

confidence: 99%

Live imaging of rapid chromosome movements in meiotic prophase I in maize

Sheehan

Pawlowski

2009

Proc. Natl. Acad. Sci. U.S.A.

110

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The ability of chromosomes to move across the nuclear space is essential for the reorganization of the nucleus that takes place in early meiotic prophase. Chromosome dynamics of prophase I have been studied in budding and fission yeasts, but little is known about this process in higher eukaryotes, where genomes and chromosomes are much larger and meiosis takes a longer time to complete. This knowledge gap has been mainly caused by difficulties in culturing isolated live meiocytes of multicellular eukaryotes. To study the nuclear dynamics during meiotic prophase in maize, we established a system to observe live meiocytes inside intact anthers. We found that maize chromosomes exhibited extremely dynamic and complex motility in zygonema and pachynema. The movement patterns differed dramatically between the two stages. Chromosome movements included rotations of the entire chromatin and movements of individual chromosome segments, which were mostly telomere-led. Chromosome motility was coincident with dynamic deformations of the nuclear envelope. Both, chromosome and nuclear envelope motility depended on actin microfilaments as well as tubulin. The complexity of the nuclear movements implies that several different mechanisms affect chromosome motility in early meiotic prophase in maize. We propose that the vigorous nuclear motility provides a mechanism for homologous loci to find each other during zygonema.chromosome dynamics ͉ cytogenetics ͉ meiosis ͉ cell biology I n early meiotic prophase, the nucleus undergoes a major spatial reorganization, which includes a general repositioning of chromatin and juxtaposition of homologous chromosomes (1). In many species, including maize, the nucleolus is located in the center of the nucleus during leptonema, and at the onset of zygonema, moves to a peripheral position (1, 2). Concurrently with the nucleus migration, all chromosome ends attach to the nuclear envelope (NE) and cluster on a single site forming the ''telomere bouquet'' (3, 4), which has been observed in most plants, animals, and fungi, including budding and fission yeasts, mouse, and maize. The telomeres remain clustered throughout zygonema. When the telomeres are clustered, centromeres are oriented in the opposite direction than the telomeres, resulting in a telomere-centromere polarization of the meiocyte nucleus. The presence of the bouquet coincides with pairing of homologous chromosomes (3, 5). In plants, mammals, and fungi, chromosome pairing depends upon the progression of meiotic recombination (5-7). However, a recombination-driven homology recognition mechanism can only operate across a relatively short distance, probably Ϸ1.2 m (6). In large-genome species, such as maize, where the zygotene nucleus is Ϸ20 m in diameter, this mechanism may not be sufficient to reach across the chromatin mass in the nucleus, even when the chromosomes are brought together by the bouquet (6). These constraints suggest that homologous chromosome segments must first be positioned close to each other, before the homology search...

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Chromosome Pairing: Effect of Colchicine on an Isochromosome

Cited by 83 publications

References 3 publications

Cryptic homoeology analysis in species and hybrids of genus Zea

Cryptic homoeology analysis in species and hybrids of genus Zea

Chromosome Organization and Dynamics during Interphase, Mitosis, and Meiosis in Plants

Live imaging of rapid chromosome movements in meiotic prophase I in maize

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