Chromosome Painting in Biological Dosimetry: Assessment of the Ability to Score Stable Chromosome Aberrations Using Different Pairs of Paint Probes

Abstract

We exposed human peripheral lymphocytes in vitro to 0.3 and 1 Gy of 6OCo gamma rays to evaluate whether the ability and sensitivity to detect chromosomal aberrations by chromosome painting is independent or not to the specific paint probes. To detect structural aberrations (translocations), we painted chromosome spreads simultaneously with two whole-chromosome libraries for chromosomes 1, 2, 3, 4, 5, 6, 7, 11, 13, 16, and 18. To compare the rate of chromosome translocations detected by the different pairs of chromosomes, data were normalized according to the fraction of genome painted and evaluated by unconditional logistic regression. Our results show that any combination of paint probes can be used to score induced chromosomal aberrations. We observed that the amounts of translocations are dose dependent and quite homogeneous within each dose of radiation, independently of chromosomes painted. However, the use of small chromosome probes is not recommended because of the high number of cells to be analyzed due to the small amount of genome painted and because it is more difficult to detect translocations in small chromosomes. Environ Health Perspect 1 04(Suppl 3): 475-477 (1996)

Indirect Genotoxic Effect of Gamma Rays in Human Peripheral Lymphocytes.

“…Gamma rays directly induced the CA in human lymphocytes (Brandriff et al 1988, Lefrancois et al 1989, Lijima and Morimoto 1991, Milkovic-Kraus et al 1992, Huber et al 1992, Kosaka et al . 1995, Sing et al 1995, Kasai et al 1996, Chambrette et al 1996, Garcia-Sagredo et al 1996, in human chorionic villi (Salvi et al 1993), in Macaca mulatta lymphocytes (Guedeney et al 1988), in cynomolgus peripheral lymphocytes (Guedeney et al 1989), in rhesus monkey (Van Buul 1989) and in mouse (Sjoblom et al 1995, Zang 1995. It is shown that gamma rays directly and indicrectly affected the DNA and caused to chromosome mutation.…”

Standardisation and Validation of Cytogenetic Markers to Quantify Radiation Absorbed Dose

“…Generally, the dose was estimated either by measuring the TL in entire genome using GTG banding 15 or FISH with a few painted chromosomes 16,17 . The concern in the TL scoring with FISH is that, extrapolation of genomic TL with a few painted chromosomes to the entire genome is based on assumptions 9 , though literature shows evidence that radiation-induced TL are distributed randomly 36 as well as non-randomly 37 . Hence, the TL frequency was examined in the PBL exposed in vitro to g-radiation, through WCP with two chromosomes, GTG banding as well as m-FISH to validate FISH with selective painting of a few chromosomes for biological dosimetry.…”