Chromosome numbers in the genus Lippia (Verbenaceae)

Viccini, Lyderson Facio; Pierre, Patrícia Maria Oliveira; Praça, Milene Miranda; Costa, Deliane Cristina; Romanel, Elisson; Sousa, Saulo Marçal de; Peixoto, Paulo Henrique Pereira; Salimena, Fátima Regina Gonçalves

doi:10.1007/s00606-005-0351-3

Cited by 42 publications

(47 citation statements)

References 8 publications

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“…The smallest diploid chromosome number described for this genus is 2n=20, suggesting that the basic chromosome number for the genus is smaller than ten (Viccini et al 2006).…”

Section: Discussionmentioning

confidence: 86%

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Pierre

Sousa

Davide

et al. 2011

An. Acad. Bras. Ciênc.

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Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of mixoploidy. Our data indicates that sexual reproduction among those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.

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“…The smallest diploid chromosome number described for this genus is 2n=20, suggesting that the basic chromosome number for the genus is smaller than ten (Viccini et al 2006).…”

Section: Discussionmentioning

confidence: 86%

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Pierre

Sousa

Davide

et al. 2011

An. Acad. Bras. Ciênc.

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“…Slides for meiotic analysis were prepared from cell suspensions, according to Viccini et al (2005), with modifi cations in the enzymatic digestion of anthers. This step was adjusted for Crotalaria using Pectinex Ultra SP-L (Novozymes), at 34°C, in a water bath, for four to six hours.…”

Section: Methodsmentioning

confidence: 99%

Abnormal meiotic behavior in three species of Crotalaria

Ferreira

Torres

Carvalho

et al. 2009

Pesq. agropec. bras.

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-The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fl uorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confi rming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and zygotene. Pollen grains of all three species show low viability, which may be associated with the irregularities of the meiotic behavior.Index terms: Crotalaria, cytogenetics, cytomixis, meiosis, pollen grain viability. Comportamento meiótico anormal em três espécies de CrotalariaResumo -O objetivo deste trabalho foi comparar o comportamento meiótico e a viabilidade dos grãos de pólen de três espécies de Crotalaria. A análise meiótica foi realizada por meio da técnica de secagem ao ar. A viabilidade dos grãos de pólen foi avaliada por testes de coloração (corante de Alexander, cloreto de tetrazólio e diacetato de fl uoresceína) e por teste de germinação em solução de sacarose. Foram observados oito bivalentes, confi rmando relatos prévios em populações de outras regiões do Brasil e de outros países. As três espécies apresentaram comportamento meiótico irregular: em Crotalaria micans, citomixia e pareamento irregular na diacinese; em C. spectabilis, pareamento irregular no diplóteno; e em C. zanzibarica, núcleo fortemente condensado nas fases de leptóteno e zigóteno. A viabilidade dos grãos de pólen das três espécies é baixa, o que pode estar associado às irregularidades do comportamento meiótico.Termos para indexação: Crotalaria, citogenética, citomixia, meiose, viabilidade do grão de pólen.

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“…Although many species of this genus are important sources of chemistry constituents for pharmaceutical, food and cosmetic industry, few studies have been done in order to investigate biological aspects of those plants (Pascual et al 2001). Taxonomically, many problems regarding the species delimitation have been reported due to the lack of good taxonomic characters and several synonymies, homonymies and infraspecific taxa (Salimena and Romero 1999, Salimena and Silva 2002, Viccini et al 2005. Therefore, revisions using additional data are necessary to better characterize Lippia species.…”

Section: Introductionmentioning

confidence: 99%

Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

Sousa

Pierre

Torres

et al. 2013

An. Acad. Bras. Ciênc.

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The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri-and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia may be used as an additional taxonomic character of the genus.

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Chromosome numbers in the genus Lippia (Verbenaceae)

Cited by 42 publications

References 8 publications

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Abnormal meiotic behavior in three species of Crotalaria

Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

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