Chromosome Microdissection and Microcloning: Technique and Application in the Plant Sciences

Sheng, Maoyin; Gao, Mengdi; Wang, Linjiao; Ren, Xuelian

doi:10.1508/cytologia.85.93

Cited by 2 publications

(3 citation statements)

References 35 publications

(66 reference statements)

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“…This implies that the Btr-1 and Btr-2 might be more strongly conserved in the 3St and 3P chromosomes. Moreover, gene cloning may be facilitated by genome dissection during flow sorting or micro dissection (Tiwari et al 2015;Said et al 2018;Sheng et al 2020;Soares et al 2020). In this study, the chromosomes belong to the homoeologous group 3 were clearly identified.…”

Section: Discussionmentioning

confidence: 74%

“…Alien gene transfer by interspecific hybridization is strongly affected by chromosome collinearity (Friebe et al 1996;Qi et al 2007). Targeted gene cloning may be facilitated by capturing the syntenic chromosomes of the wild relatives, which include complex genomes, by flow sorting (Tiwari et al 2015;Said et al 2018) or even by micro dissection (Sheng et al 2020;Soares et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

Tao¹,

Liu

Dou

2021

CCG

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Kengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed using 14 single-gene probes covering all seven homoeologous groups, and four repetitive sequence probes 45S rDNA, 5S rDNA, pAs1, and (AAG)10 by FISH. Each chromosome of K. hirsuta was well characterized by homoeological determination and repeats distribution patterns. The synteny of chromosomes was strongly conserved in the St genome, whereas synteny of the Y and P genomes was more distorted. The collinearity of 1Y, 2Y, 3Y and 7Y might be interrupted in the Y genome. A new 5S rDNA site on 2Y might be translocated from 1Y. The short arm of 3Y might involve translocated segments from 7Y. The 7 Y was identified as involving a pericentric inversion. A reciprocal translocation between 2P and 4P, and tentative structural aberrations in the subtelomeric region of 1PL and 4PL, were observed in the P genome. Chromosome polymorphisms, which were mostly characterized by repeats amplification and deletion, varied between chromosomes, genomes, and different populations. However, two translocations involving a P genome segmental in 3YL and a non-Robertsonial reciprocal translocation between 4Y and 3P were identified in two independent populations. Moreover, the proportion of heterozygous karyotypes reached almost 35% in all materials, and almost 80% in the specific population. These results provide new insights into the genome organization of K. hirsuta and will facilitate genome dissection and germplasm utilization of this species.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

Tao¹,

Liu

Dou

2021

CCG

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“…Chromosome specimen preparation is fundamental for several aspects of cytology, including karyotyping, fluorescence in situ hybridization (FISH), and chromosome microdissection (Bhowmick Biplab and Jha 2019, Kono et al 2019, Dash Chandan et al 2020, Nam et al 2020, Saensouk and Saensouk 2020, Samaropoulou et al 2020, Santra et al 2020, Shafiee et al 2020, Sheng et al 2020, Suto et al 2020, Widarmi et al 2020, Wang et al 2021. These techniques revealed differences in chromosome architecture between species, chromosomal abnormalities associated with diseases, and the roles of chromosomes in sex determination.…”

mentioning

confidence: 99%

Effective Chromosomal Preparation Protocol for the Dioecious Plant <i>Silene latifolia</i>

et al. 2021

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Cytology requires chromosome specimens, thus, effective preparation methods are needed. Chromosome specimens are frequently prepared from plant root tips. Furthermore, cell cycle synchronization using chemical reagents is applied to obtain a large number of metaphase chromosome specimens. In this study, we focused on the timing of root tip sampling, which is optimal for the preparation of chromosome specimens of the dioecious plant Silene latifolia. The timing was determined from the time seeds were subjected to a germination treatment. Observation of metaphase chromosomes using microscopy revealed that the number of mitotic cells peaked 54 h after the germination treatment. This trend was also observed when the DNA synthesis inhibitor aphidicolin was administered from 24 to 9 h before sampling time points. We used ice-cold treatment for 8, 16, and 32 h as a chromosome condensation method. The 16 h treatment produced suitable chromosome specimens showing satellite ends of chromosomes, whereas the 32 h treatment produced well-condensed chromosome specimens, which were suitable for counting chromosome numbers. Our findings suggest that the timing of root tip sampling is essential for effectively producing plant chromosome specimens.

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Chromosome Microdissection and Microcloning: Technique and Application in the Plant Sciences

Cited by 2 publications

References 35 publications

The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

Effective Chromosomal Preparation Protocol for the Dioecious Plant <i>Silene latifolia</i>

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