Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation

Coluccia, Elisabetta; Deidda, Federica; Lobina, Cinzia; Melis, Riccardo; Porcu, Cristina; Agus, Blondine; Salvadori, Susanna

doi:10.3390/genes11111319

Cited by 3 publications

(3 citation statements)

References 49 publications

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“…An interstitial 5S rDNA position with a diverse location is current, presumably because of a thin inversion. A plus 5S rDNA likely implies the presence of a replication (Coluccia et al 2020). The 5S rDNA site of one parent was either excluded from the chromosome or shifted into gene silencing and then disappeared, which could decrease the 5S rDNA site number (de Melo and Guerra 2003, Volkov et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Luo,

Liu,

Gong

et al. 2024

Preprint

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5S rDNA is essential component of all cell types. A survey was conducted to study the 5S rDNA site number, position, and origin of signal pattern diversity in 64 plants using fluorescencein situhybridization. The species used for this experiment were chosen due to the discovery of karyotype rearrangement, and for species checked in which 5S rDNA has not yet been explored. The chromosome number was 14160, while the chromosome length was 0.636.88 m, with 37 plants (58%) had small chromosome (<3 m). The chromosome numbers of three species and 5S rDNA loci of 19 species have been reported for the first time. 5S rDNA was varied and abundant in signal site number (218), position (e.g., interstitial, distal, and proximal position, occasionally, outside chromosome), and even as intense (e.g., strong, weak, and slight). Our results exposed modifiability in the number and location of 5S rDNA in 33 plants and demonstrated an extensive stable number and location of 5S rDNA in 31 plants. The potential origin of signal pattern diversity was probably caused by chromosome rearrangement (e.g., deletion, duplication, inversion, translocation), polyploidization, self-incompatibility, and chromosome satellites. These data characterized the variability of 5S rDNA within the karyotypes of the 64 plants that exhibited massive chromosomal rearrangements and provided anchor points for genetic physical maps. These data prove the utility of the 5S rDNA oligonucleotide as a chromosome marker in identifying plant chromosomes. This method provides a basis for developing similar applications for cytogenetic analysis in other species.

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Section: Discussionmentioning

confidence: 99%

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Luo,

Liu,

Gong

et al. 2024

Preprint

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show abstract

“…An interstitial 5S rDNA position with a diverse location is presumed to indicate a thin inversion. A plus 5S rDNA implies the presence of a replication [81]. In addition, the 5S rDNA site of one parent was either excluded from the chromosome or shifted into gene silencing and then disappeared, which could decrease the 5S rDNA site number [82,83].…”

Section: Potential Origin Of 5s Rdna Diversity In Plantsmentioning

confidence: 99%

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 42 Species

Luo,

Liu,

Gong

et al. 2024

Genes

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This study was conducted to evaluate the 5S rDNA site number, position, and origin of signal pattern diversity in 42 plant species using fluorescence in situ hybridization. The species were selected based on the discovery of karyotype rearrangement, or because 5S rDNA had not yet been explored the species. The chromosome number varied from 14 to 160, and the chromosome length ranged from 0.63 to 6.88 μm, with 21 species having small chromosomes (<3 μm). The chromosome numbers of three species and the 5S rDNA loci of nineteen species are reported for the first time. Six 5S rDNA signal pattern types were identified. The 5S rDNA varied and was abundant in signal site numbers (2–18), positions (distal, proximal, outside of chromosome arms), and even in signal intensity. Variation in the numbers and locations of 5S rDNA was observed in 20 species, whereas an extensive stable number and location of 5S rDNA was found in 22 species. The potential origin of the signal pattern diversity was proposed and discussed. These data characterized the variability of 5S rDNA within the karyotypes of the 42 species that exhibited chromosomal rearrangements and provided anchor points for genetic physical maps.

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“…Finally, chromosome mapping of major and minor ribosomal gene clusters was applied in a comparative cytogenetic analysis of Muraeinidae, an ancient and poorly studied family. Results unveiled a pattern of ribosomal sequence distribution in contrast to what was generally reported for fishes, showing high variability in the number and location of 5S rDNA clusters, and a tendency to the conservation of the number and location of the 45S rDNA clusters among species [ 22 ].…”

mentioning

confidence: 99%

Fish Cytogenetics: Present and Future

Rossi

2021

Genes

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Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation

Cited by 3 publications

References 49 publications

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 42 Species

Fish Cytogenetics: Present and Future

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