Chromosome location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L.) 1. Mlk and other alleles at the Pm3 locus

Zeller, F. J.; Lutz, J.; Stephan, U.

doi:10.1007/bf00029876

Cited by 57 publications

(43 citation statements)

References 15 publications

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“…Chinese Spring and Kanzler are generally susceptible to powdery mildew, and powdery mildew isolates are regularly propagated on line Kanzler (Zeller et al, 1993). If functional Pm3 alleles are present in these two lines, these genes were possibly overcome by virulent pathogen races and are no longer efficient against any of the tested isolates.…”

Section: Identification Of a Pm3 Haplotype In Different Wheat Lines/cmentioning

confidence: 99%

“…With the exception of Pm3h, which might have originated from a cultivated T. durum Ethiopian line, the active Pm3 alleles have all been found in hexaploid bread wheat lines (Hsam and Zeller, 2002). In addition, the alleles were first characterized in lines from different gene pools originating from all continents where wheat is grown (Briggle and Sears, 1966;Zeller et al, 1993). This suggests that they have evolved independently from each other in different environments and breeding conditions.…”

Section: Evolution Of the Pm3 Locusmentioning

confidence: 99%

“…The Pm3 alleles have been found in wheat lines or cultivars originating from the five continents, and a number of them have been widely used in wheat breeding in Europe (Pm3a, Pm3d) and in the United States (Pm3a; Hsam and Zeller, 2002). The Pm3 alleles have been characterized based on classical allelic tests (Zeller et al, 1993;Zeller and Hsam, 1998). There, distinct genes might be misclassified as allelic due to the low genetic recombination between them.…”

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confidence: 99%

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Allelic Series of Four Powdery Mildew Resistance Genes at the Pm3 Locus in Hexaploid Bread Wheat

et al. 2005

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At the Pm3 locus in hexaploid wheat (Triticum aestivum), 10 alleles conferring race-specific resistance to powdery mildew (Blumeria graminis f. sp. tritici) are known. A cluster of genes encoding coiled-coil-nucleotide-binding site-leucine-rich repeat proteins spans the Pm3 locus on wheat chromosome 1A, and one member of this gene family has recently been identified as the Pm3b resistance gene. Using molecular markers closely linked to Pm3b, we performed haplotype analysis of 10 lines carrying different Pm3 alleles. All these lines have a conserved genomic region delimited by markers cosegregating with Pm3b and including a structurally conserved Pm3b-like gene. A polymerase chain reaction-based strategy allowed the amplification of one Pm3b-like sequence from lines carrying Pm3a, Pm3d, and Pm3f alleles. These candidate genes for Pm3a, Pm3d, and Pm3f conferred AvrPm3a-, AvrPm3d-, and AvrPm3f-dependent resistance, respectively, to wheat powdery mildew in a single cell transient transformation assay. A high level of amino acid similarity (97.8%) was found between the PM3A, PM3B, PM3D, and PM3F proteins. The coiled-coil domain was 100% conserved, whereas, in the nucleotide binding site region, sequence exchange was detected, indicating intragenic recombination or gene conversion between alleles. All these results indicate that Pm3a, Pm3b, Pm3d, and Pm3f form a true allelic series. The low level of sequence divergence between the four characterized alleles as well as the finding of a conserved Pm3 haplotype are in agreement with the hypothesis of a recent evolution of Pm3-based resistance, suggesting that some or most of the diversity found at the Pm3 locus in modern wheat has evolved after wheat domestication.

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Section: Identification Of a Pm3 Haplotype In Different Wheat Lines/cmentioning

confidence: 99%

Section: Evolution Of the Pm3 Locusmentioning

confidence: 99%

mentioning

confidence: 99%

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Allelic Series of Four Powdery Mildew Resistance Genes at the Pm3 Locus in Hexaploid Bread Wheat

et al. 2005

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“…The tests were conducted on first leaf segments placed on 5.3 g/l agar media containing 35 mg/l benzimidazole in Petri dishes. Mildew infections were carried out according to the method described by Zeller et al (1993a). Plants were scored on a 0-9 scale (0 ¼ no visible sporulation, 9 ¼ heavy sporulation).…”

Section: Methodsmentioning

confidence: 99%

“…Pm3e and Pm3f are ineffective in France. Pm3g, previously described in the cultivar ÔAristideÕ and named Mlar (Zeller et al 1993a) is also ineffective. The effectiveness of Pm3g in regions outside western Europe is unknown but it could be widely effective.…”

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confidence: 99%

Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat

Bougot¹,

Lemoine²,

Pavoine³

et al. 2002

Plant Breeding

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The Pm3 resistance locus, located on chromosome 1A in wheat, confers race-specific resistance to the obligate biotrophic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, the causal agent of powdery mildew. Several Pm3 alleles are still effective in controlling the disease in Europe. A genetic map was constructed to map the Pm3g allele in the recombinant inbred line progeny from the cross ÔRE9001Õ (susceptible) · ÔCourtotÕ (resistant). Two microsatellite markers were closely mapped to Pm3g. The PSP2999 marker, which cosegregates with this allele, was shown to detect the presence of the Pm3g resistance allele in other cultivars. A collection of 56 wheat cultivars or advanced lines carrying one Pm3 allele was used to assess the allelespecific amplification of the PSP2999 marker. The same amplification pattern was obtained for lines with Pm3a, Pm3b, Pm3e, Pm3f and Pm3g alleles. Twenty genotypes carrying Pm3d showed a specific amplification pattern. This marker allowed the detection of the Pm3d allele in highly resistant lines whose resistance gene combinations were unknown. It was concluded that PSP2999 is a useful marker to detect Pm3 alleles in parents and to manage them in breeding programmes.

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Identification and Implementation of Resistance: Genomics-Assisted use of Genetic Resources for Breeding Against Powdery Mildew and Stagonospora Nodorum Blotch in Wheat

Selter

Shatalina

Singla

et al. 2013

Genomics of Plant Genetic Resources

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Chromosome location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L.) 1. Mlk and other alleles at the Pm3 locus

Cited by 57 publications

References 15 publications

Allelic Series of Four Powdery Mildew Resistance Genes at the Pm3 Locus in Hexaploid Bread Wheat

Allelic Series of Four Powdery Mildew Resistance Genes at the Pm3 Locus in Hexaploid Bread Wheat

Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat

Identification and Implementation of Resistance: Genomics-Assisted use of Genetic Resources for Breeding Against Powdery Mildew and Stagonospora Nodorum Blotch in Wheat

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