Chromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat

Jimenez, Monika K. Michalak de; Dubcovsky, Jorge

doi:10.1046/j.1439-0523.1999.00393.x

Cited by 61 publications

(49 citation statements)

References 11 publications

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“…These genes, PPO-A1 and PPO-D1, have been shown to affect kernel PPO activity and noodle dough color stability (Chang et al 2007;He et al 2007;Sun et al 2005). PPO-A1 and PPO-D1 are located on the long arm of the homeologous chromosomes 2A and 2D, respectively (Anderson et al 2006;Chang et al 2007;Demeke et al 2001;He et al 2007;Jimenez and Dubcovsky 1999;Mares and Campbell 2001;Raman et al 2005;Simeone et al 2002;Sun et al 2005;Watanabe et al 2004;Zhang et al 2005). Recently, another paralogous PPO gene family has been identified and consists of the PPO-A2, PPO-B2, and PPO-D2 genes (Beecher and Skinner 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.)

2012

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Polyphenol oxidase (PPO) enzymatic activity is a major cause in time-dependent discoloration in wheat dough products. The PPO-A1 and PPO-D1 genes have been shown to contribute to wheat kernel PPO activity. Recently a novel PPO gene family consisting of the PPO-A2, PPO-B2, and PPO-D2 genes has been identified and shown to be expressed in wheat kernels. In this study, the sequences of these five kernel PPO genes were determined for the spring wheat cultivars Louise and Penawawa. The two cultivars were found to be polymorphic at each of the PPO loci. Three novel alleles were isolated from Louise. The Louise X Penawawa mapping population was used to genetically map all five PPO genes. All map to the long arm of homeologous group 2 chromosomes. PPO-A2 was found to be located 8.9 cM proximal to PPO-A1 on the long arm of chromosome 2A. Similarly, PPO-D1 and PPO-D2 were separated by 10.7 cM on the long arm of chromosome 2D. PPO-B2 mapped to the long arm of chromosome 2B and was the site of a novel QTL for polyphenol oxidase activity. Five other PPO QTL were identified in this study. One QTL corresponds to the previously described PPO-D1 locus, one QTL corresponds to the PPO-D2 locus, whereas the remaining three are located on chromosome 2B.

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Section: Introductionmentioning

confidence: 99%

Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.)

2012

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“…Previous studies in wheat have shown that major genes controlling PPO activity are located on wheat homeologous group 2 chromosomes (Udall 1996;Jimenez and Dubcovsky 1999;Anderson and Morris 2001;Demeke et al 2001;Mares and Campbell 2001), but activity might be modulated or influenced by additional genes on group 3 (Udall 1996;Demeke et al 2001), group 5 (Udall 1996), 6B and 7D chromosomes (Li et al 1999). Raman et al (2005) identified a major locus controlling PPO activities on the long arm (2AL) of chromosome 2A in a doubledhaploid population derived from Chara/WW2449, explaining 82-84 % of the genetic variation, using a QTL mapping approach.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of grain polyphenol oxidase (PPO) activity in multiple wheat (Triticum aestivum L.) genetic backgrounds

2012

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Grain polyphenol oxidase (PPO) activity can cause discoloration of wheat (Triticum aestivum L.) food products. Five crosses (PI 117635/Antelope; Fielder/ NW03681; Fielder/Antelope; NW07OR1070/Antelope; NW07OR1066/OR2050272H) were selected to study the genetic inheritance of PPO activity. STS markers, PPO18, PPO29 and STS01, were used to identify lines with putative alleles at the Ppo-A1 and Ppo-D1 loci conditioning low or high PPO activity. ANOVA showed significant genotypic effects on PPO activity (P \ 0.0001) in all populations. The generations and generation 9 genotype effects were not significant in any population. A putative third (null) genotype at Ppo-A1 (no PCR fragments for PPO18) was discovered in NW07OR1066 and NW07OR1070 derived populations, and these had the lowest mean PPO activities. Results demonstrated that both Ppo-A1 and Ppo-D1 loci affect the kernel PPO activity, but the Ppo-A1 has the major effect. In three populations, contrary results were observed to those predicted from previous work with Ppo-D1 alleles, suggesting the markers for Ppo-D1 allele might give erroneous results in some genetic backgrounds or lineages. Results suggest that selection for low or null alleles only at Ppo-A1 might allow development of low PPO wheat cultivars.

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“…In hexaploid wheat, the 2D chromosome was associated with enhanced levels of kernel PPO activity and in tetraploid wheat, which lacks the D genome, high PPO activity was mapped to the long arm of chromosome 2A (Simone et al 2002;Raman et al 2005). Jiménez and Dubcovsky (1999), using chromosome substitution lines from three cultivars into Chinese Spring, proposed that genes located on homoeologous group 2 of wheat chromosomes play a major role in PPO activity. QTL analysis showed that additional genes influencing PPO activity might be located on chromosomes 2A, 2B, 2D, 3D and 6B (Jiménez and Dubcovsky 1999;Demeke et al 2001;Simone et al 2002;Raman et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Jiménez and Dubcovsky (1999), using chromosome substitution lines from three cultivars into Chinese Spring, proposed that genes located on homoeologous group 2 of wheat chromosomes play a major role in PPO activity. QTL analysis showed that additional genes influencing PPO activity might be located on chromosomes 2A, 2B, 2D, 3D and 6B (Jiménez and Dubcovsky 1999;Demeke et al 2001;Simone et al 2002;Raman et al 2005). Beecher and Skinner (2011) used real-time PCR analysis to demonstrate that Ppo-A1a, Ppo-A2b, Ppo-D1b and Ppo-D2b in the wheat cultivar 'Alpowa' were expressed to substantial levels in developing wheat kernels, whereas Ppo-B1 and Ppo-B2 expression was not detected.…”

Section: Introductionmentioning

confidence: 99%

Enzyme activity in wheat breeding lines derived from matings of low polyphenol oxidase parents

2012

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Polyphenol oxidase (PPO) in grain plays a major role in time-dependent discoloration of wheat (Triticum aestivum L.) products, especially fresh noodles. Breeding wheat cultivars with low or nil PPO activity can reduce undesirable product darkening. The low PPO line PI 117635 was crossed to two low PPO wheats, IDO580 and 'IDO377s', to determine whether matings between wheats with low levels of grain PPO would result in complementation, such that lines with still lower or nil PPO would be generated. Progeny in a population derived from PI 117635/ IDO580 displayed no variation in PPO activity. In the F 3:4 populations derived from PI 117635/IDO377s, and the reciprocal IDO377s/PI 117635, normal distributions of low to high PPO activity were observed. Fieldgrown populations (F 3:5 ; F 3:6 ) derived from IDO377s crosses were analyzed for PPO activity and used to determine whether lines with nil PPO activity were generated. Of 239 lines, 154 were verified to have PPO activity that was not significantly different from the low PPO durum (Triticum turgidum var durum) cultivar 'Ben'. PCR analysis showed that the populations were fixed for a putative low PPO allele at Ppo-A1. Using markers for Ppo-D1, it was found that the average PPO activity of lines with 490 bp PCR fragments from PPO29 was significantly lower than that of lines with 560 bp fragments from STS01. These results disagreed with that predicted from previous reports for markers for Ppo-D1 alleles. Thus, breeders should exercise caution when making selections using markers for alleles at Ppo-D1, as known markers might predict erroneous phenotypes and genotypes in some wheat backgrounds.

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Chromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat

Abstract: TitleChromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat Permalink https://escholarship.org/uc/item/3bt9h1r5 Plant Breeding, 118(5) Journal

Cited by 61 publications

References 11 publications

Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.)

Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.)

Inheritance of grain polyphenol oxidase (PPO) activity in multiple wheat (Triticum aestivum L.) genetic backgrounds

Enzyme activity in wheat breeding lines derived from matings of low polyphenol oxidase parents

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