Chromosome Instability in 'Carrizo' Citrange + Citrus Macrophylla Somatic Hybrids

Abstract

S05sour orange seedlings. The embryogenic callus and leaf midveins of 3 month-old regenerated plants were cultured in SP4 and LD8 media, which were used for PCR assays. Results of sanitary tests showed a 30% infection rate in the callus while no infection was detected in the regenerated plants. S05P27The study on callus induction of citrus anther culture in vitro

Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks

“…Two allotetraploid somatic hybrids were previously obtained from protoplasts isolated from callus of CM and from leaves of CC (Pensabene-Bellavia et al, 2015 ). These somatic hybrids, their parents, and the embryogenic callus of CM were analyzed to verify their origin and genetic structure of the hybrids.…”

“…Diploid CC and CM and allotetraploid somatic hybrids SMC-58 and SMC-73 were used for the experiments. Diploid CC and CM seeds were collected from the Citrus Germplasm Bank of pathogen-free plants at the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Navarro et al, 2002 ; Navarro, 2015 ) and somatic hybrids SMC-58 and SMC-73 were recovered by protoplast fusion isolated from CM embryogenic callus and CC leaf mesophyll leaves (Pensabene-Bellavia et al, 2015 ). The somatic hybrids were micropropagated by Agromillora Research S.L.…”

“…However, in vitro micropropagation allows the generation of a large number of clonal plants in a short time, avoiding the delay that juvenility would impose, which is a great advantage for rootstock breeding programs (Bordas et al, 2015 ). In this study, the genetic composition of two somatic hybrids, obtained by CM and CC protoplast fusion (Pensabene-Bellavia et al, 2015 ), was analyzed using single sequence repeats (SSR), single nucleotide polymorphism (SNP), and insertion or deletion (InDel) markers. Both somatic hybrids were morphologically described and their behavior was evaluated under salinity, iron deficiency, and CTV inoculation.…”

Identificación de caracteres fisiológicos y moleculares para la tolerancia a estreses abióticos en portainjertos de cítricos tetraploides.

“…I.4B.) o con adiciones asimétricas del genoma nuclear (Gmitter et al, 1992;Miranda et al, 1997;Pensabene-Bellavia et al, 2015) y su origen se atribuye a la inestabilidad que los genomas sufren durante el proceso de fusión y regeneración. Este fenómeno ha sido descrito también en híbridos somáticos de Brassica spp., Hordeum vulgare L., Daucus carota L. y Solanum tuberosum L., en los que las pérdidas cromosómicas durante el proceso se correlacionan directamente con la distancia genética existente entre los parentales (Sundberg y Glimelius, 1991;Kisaka et al, 1997;Oberwalder et al, 1998).…”

“…Se utilizaron plantas 2x de CC y CM, obtenidas a partir de semilla procedente de árboles del Banco de Germoplasma (BG) de plantas libres de patógenos del Instituto Valenciano de Investigaciones Agrarias (IVIA) Navarro, 2015) y plantas micropropagadas de los HS alotetraploides SMC-58 y SMC-73, obtenidos en el IVIA mediante fusión de protoplastos de callo embriogénico de CM y mesófilo de hoja de CC (Pensabene-Bellavia et al, 2015). Los HS se micropropagaron por Agromillora Research S.L.…”