Chromosome Healing Is Promoted by the Telomere Cap Component Hiphop in<i>Drosophila</i>

Kurzhals, Rebeccah L.; Fanti, Laura; Ebsen, A C Gonzalez; Rong, Yikang S.; Pimpinelli, Sergio; Golic, Kent G.

doi:10.1534/genetics.117.300317

Cited by 18 publications

(15 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the centric fragment to be adequately maintained, its broken end would need to be mitigated, such as through the formation of a ring chromosome that would negate a need for telomeres altogether (Titen and Golic 2008). Alternatively, the broken end may become a functional neo-telomere that can recruit telomeric capping proteins, which has previously been observed in Drosophila (Gao et al 2010; Kurzhals et al 2017).…”

Section: Resultsmentioning

confidence: 82%

Origin, Composition, and Structure of the Supernumerary B Chromosome of Drosophila melanogaster

et al. 2018

View full text Add to dashboard Cite

The number of chromosomes carried by an individual species is one of its defining characteristics. Some species, however, can also carry supernumerary chromosomes referred to as B chromosomes. B chromosomes were recently identified in a laboratory stock of -an established model organism with a wealth of genetic and genomic resources-enabling us to subject them to extensive molecular analysis. We isolated the B chromosomes by pulsed-field gel electrophoresis and determined their composition through next-generation sequencing. Although these B chromosomes carry no known euchromatic sequence, they are rich in transposable elements and long arrays of short nucleotide repeats, the most abundant being the uncharacterized satellite repeat. Fluorescent hybridization on metaphase chromosome spreads revealed this repeat is located on chromosome, strongly suggesting the origin of the B chromosomes is chromosome Cytological and quantitative comparisons of signal intensity between chromosome and the B chromosomes supports the hypothesis that the structure of the B chromosome is an isochromosome. We also report the identification of a new B chromosome variant in a related laboratory stock. This B chromosome has a similar repeat signature as the original but is smaller and much less prevalent. We examined additional stocks with similar genotypes and did not find B chromosomes, but did find these stocks lacked the satellite repeat. Our molecular characterization of B chromosomes is the first step toward understanding how supernumerary chromosomes arise from essential chromosomes and what may be necessary for their stable inheritance.

show abstract

Section: Resultsmentioning

confidence: 82%

Origin, Composition, and Structure of the Supernumerary B Chromosome of Drosophila melanogaster

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Additionally, two of the three telomere repeat additions involved invasion or fusion with other sequences before the addition of repeat sequences. De novo telomere formation on broken DNA ends has been observed in many species and is commonly known as chromosome healing (45)(46)(47). Chromosome healing occurs during mammalian development and has been observed in mouse embryonic stem cells as well as human germline cells (48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

Centromere scission drives chromosome shuffling and reproductive isolation

Yadav

Sun

Coelho

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A fundamental characteristic of eukaryotic organisms is the generation of genetic variation via sexual reproduction. Conversely, significant large-scale genome structure variations could hamper sexual reproduction, causing reproductive isolation and promoting speciation. The underlying processes behind large-scale genome rearrangements are not well understood and include chromosome translocations involving centromeres. Recent genomic studies in theCryptococcusspecies complex revealed that chromosome translocations generated via centromere recombination have reshaped the genomes of different species. In this study, multiple DNA double-strand breaks (DSBs) were generated via the CRISPR/Cas9 system at centromere-specific retrotransposons in the human fungal pathogenCryptococcus neoformans. The resulting DSBs were repaired in a complex manner, leading to the formation of multiple interchromosomal rearrangements and new telomeres, similar to chromothripsis-like events. The newly generated strains harboring chromosome translocations exhibited normal vegetative growth but failed to undergo successful sexual reproduction with the parental wild-type strain. One of these strains failed to produce any spores, while another produced ∼3% viable progeny. The germinated progeny exhibited aneuploidy for multiple chromosomes and showed improved fertility with both parents. All chromosome translocation events were accompanied without any detectable change in gene sequences and thus suggest that chromosomal translocations alone may play an underappreciated role in the onset of reproductive isolation and speciation.

show abstract

“…For this mutation to be stable, that is, an inheritable chromosome-number increase by one ( n + 1), the compromised centromeres should retain the capacity to form a kinetochore and the centric ends of the two telocentric chromosomes should be healed by de novo telomere formation ( Jankowska et al. 2015 ; Kurzhals et al. 2017 ).…”

Section: Processes Governing Chromosome-number Variationmentioning

confidence: 99%

The Evolution of Chromosome Numbers: Mechanistic Models and Experimental Approaches

Mayrose

Lysak

2020

Genome Biology and Evolution

View full text Add to dashboard Cite

Chromosome numbers have been widely used to describe the most fundamental genomic attribute of an organism or a lineage. While providing strong phylogenetic signal, chromosome numbers vary remarkably among eukaryotes at all levels of taxonomic resolution. Changes in chromosome numbers regularly serve as indication of major genomic events, most notably polyploidy and dysploidy. Here, we review recent advancements in our ability to make inferences regarding historical events that led to alterations in the number of chromosomes of a lineage. We first describe the mechanistic processes underlying changes in chromosome numbers, focusing on structural chromosomal rearrangements. Then, we focus on experimental procedures, encompassing comparative cytogenomics and genomics approaches, and on computational methodologies that are based on explicit models of chromosome-number evolution. Together, these tools offer valuable predictions regarding historical events that have changed chromosome numbers and genome structures, as well as their phylogenetic and temporal placements.

show abstract

Chromosome Healing Is Promoted by the Telomere Cap Component Hiphop inDrosophila

Cited by 18 publications

References 77 publications

Origin, Composition, and Structure of the Supernumerary B Chromosome of Drosophila melanogaster

Origin, Composition, and Structure of the Supernumerary B Chromosome of Drosophila melanogaster

Centromere scission drives chromosome shuffling and reproductive isolation

The Evolution of Chromosome Numbers: Mechanistic Models and Experimental Approaches

Contact Info

Product

Resources

About