Chromosome-Encoded CTX-M-3 from
            <i>Kluyvera ascorbata</i>
            : a Possible Origin of Plasmid-Borne CTX-M-1-Derived Cefotaximases

Rodríguez, María Margarita; Power, Pablo; Radice, Marcela; Vay, Carlos; Famiglietti, Ángela; Galleni, Moreno; Ayala, Juan A.; Gutkind, Gabriel

doi:10.1128/aac.48.12.4895-4897.2004

Cited by 120 publications

(97 citation statements)

References 18 publications

(15 reference statements)

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“…Arguably one of the most clinically important groups of β-lactamases in Gram negative bacteria at the moment are the CTX-M family (Livermore and Hawkey, 2005). The identification of environmental progenitors of the extended-spectrum β-lactamase (ESBL) CTX-M enzymes responsible for resistance to 3 rd generation cephalosporins (3GCs) in bacteria of the genus Kluyvera clearly indicates the significance of the environment in the evolution of emerging antibiotic resistance determinants (Bonnet, 2004;Rodriguez et al, 2004). Kluyvera sp.…”

Section: A Origins Of Antibiotic Resistance Genesmentioning

confidence: 99%

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Gaze

O’Neill

Wellington

et al. 2008

Advances in Applied Microbiology

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Section: A Origins Of Antibiotic Resistance Genesmentioning

confidence: 99%

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Gaze

O’Neill

Wellington

et al. 2008

Advances in Applied Microbiology

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“…Beta-Lactamases of the CTX-M group II are structurally related to the naturally produced beta-lactamase of Kluyvera ascorbata (6), and in CTX-M group III, is related to beta-lactamase of K. georgiana (7), and CTX-M group I enzymes are related to the beta-lactamases of K. cryocrescens (8). Although an identical enzyme to CTX-M-3 was isolated from K.ascorbata (9). The CTX-M group IV is related to enzymes from Kluyvera spp.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of CTX-M-Type Beta-lactamase Among Clinical Isolates of Enterobacteriaceae in Markazi Province, Iran

Safari

Shojapour

Akbari

et al. 2013

Jundishapur J Microbiol

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Background: Organisms producing CTX-M-lactamases are known as the source of resistance to Oxyiminocephalosporins such as Eeftriaxone and Ceftazidime. However, the laboratory detection of these strains is not well defined. Objectives: The aim of this study was to determine the presence and prevalence of known CTX-M-beta-beta-lactamase genes in clinical isolates of Enterobacteriaceae from Arak educational hospitals, Iran. Materials and Methods: During a 10-month period (May to February 2010), 350 randomly Enterobacteriaceae isolates were obtained from the clinical laboratories of different hospitals of Arak University of Medical Sciences, Iran. Antibiotic susceptibility was tested by CLSI disk diffusion and extended spectrum beta-lactamase (ESBL) confirmatory tests. Minimum Inhibitory Concentration (MICs) was determined by broth micro dilution. All of the ESBL-producing isolates were examined by PCR to detect the presence of bla CTX-M genes. Results: In phenotypic confirmatory test, 154 (44%) out of 350 clinical isolates were ESBL positive. Using molecular assay, 154 strains potentially producing extended-spectrum-beta -lactamases were examined for the presence of CTX-M enzymes. 92.2% isolates CTX-M -1, 28.5% isolates CTX-M-2, 17.5% isolates CTX-M-8, and 38.3% isolates CTX-M-9 genes detected by PCR. Conclusions:The levels of resistance to Ceftazidime were remarkably variable among CTX-Mproducers. This study provides further evidences of the global dissemination of CTX-M type ESBLs and emphasized on the need for their epidemiological monitoring.

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“…CTX-M type ESBLs or cefotaximases, belong to class A blactamases and are encoded by bla CTX-M genes located in a plasmid or on the chromosome (Rodriguez et al, 2004). Different elements may be involved in the mobilization of bla CTX-M genes.…”

Section: Introductionmentioning

confidence: 99%

“…Other groups, including PER and CTX-M types, have been described recently (Bonnet, 2004;Bradford, 2001). Also, b-lactamases belonging to either Ambler class B (metallo-b-lactamase) or class A, such as KPC-2, able to hydrolyse carbapenems, have been described (Miriagou et al, 2003).CTX-M type ESBLs or cefotaximases, belong to class A blactamases and are encoded by bla CTX-M genes located in a plasmid or on the chromosome (Rodriguez et al, 2004). Different elements may be involved in the mobilization of bla CTX-M genes.…”

mentioning

confidence: 99%

Emergence of CTX-M-15 type extended-spectrum β-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates

Rotimi

Jamal

Pal

et al. 2008

Journal of Medical Microbiology

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Cephalosporins are major antimicrobials used to treat serious Salmonella infections. However, their effectiveness is being compromised by the emergence of extended-spectrum b-lactamases (ESBLs). The genetic determinants encoding ESBL in Salmonella spp. isolated from patients in Kuwait and United Arab Emirates (UAE) were studied over a 2 year period. Out of a total of 407 isolates, 116 isolates possessed the resistance phenotypes consistent with possible ESBL production. Of these, 69 (59.5 %) were ESBL positive. PCR and sequencing were used to determine the genetic determinant(s) responsible for ESBL phenotypes. A total of 14 (12.1 %) and 29 (24.6 %) isolates were CTX-M-15 ESBL producers and TEM producers, respectively. Ten CTX-M-15 producers carried the insertion sequence ISEcpI gene. PFGE analysis revealed identical profiles in 4 of the 13 Kuwaiti strains. This study reports the presence of the bla CTX-M-15 gene in Salmonella spp. and Salmonella enterica serotype Typhi from Kuwait and UAE for what is believed to be the first time. This is of great concern as the gene is also found in association with the ISEcpI gene, which may easily facilitate its spread. These isolates originated mostly from nonKuwaiti Arabs rather than from people of Asian origin. INTRODUCTIONSalmonella spp. are an important cause of enteric fever and gastroenteritis in humans worldwide. The organisms are transmitted by contaminated food and inadequate hygiene. Typhoidal and serious invasive non-typhoidal Salmonella spp. infections are usually treated with antimicrobial agents. Fluoroquinolones and b-lactams are the drugs of choice for invasive salmonella infections. However, resistance has emerged to various classes of antibiotics in many parts of the world with the spread of resistant strains. Both health care-associated outbreaks and community outbreaks have been reported (Arlet et al., 2006).In Salmonella, resistance to cephalosporins is largely due to production of extended-spectrum b-lactamases (ESBLs). Most ESBLs in Salmonella are derivatives of TEM and SHV b-lactamase families. Other groups, including PER and CTX-M types, have been described recently (Bonnet, 2004;Bradford, 2001). Also, b-lactamases belonging to either Ambler class B (metallo-b-lactamase) or class A, such as KPC-2, able to hydrolyse carbapenems, have been described (Miriagou et al., 2003).CTX-M type ESBLs or cefotaximases, belong to class A blactamases and are encoded by bla CTX-M genes located in a plasmid or on the chromosome (Rodriguez et al., 2004). Different elements may be involved in the mobilization of bla CTX-M genes. Studies with plasmids have confirmed the potential involvement of the insertion sequence ISEcp1 in the mobility of bla CTX-M (Cao et al., 2002). CTX-M enzymes comprise a rapidly growing family of enzymes disseminated in several parts of the world (Bonnet, 2004). A concern is the fact that CTX-M type ESBLs display a level of resistance to cefotaxime (Ctx) and ceftriaxone (Cro) significantly higher than that to ceftazidime (Caz) (Bradford, 2001),...

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Chromosome-Encoded CTX-M-3 from Kluyvera ascorbata : a Possible Origin of Plasmid-Borne CTX-M-1-Derived Cefotaximases

Cited by 120 publications

References 18 publications

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Dissemination of CTX-M-Type Beta-lactamase Among Clinical Isolates of Enterobacteriaceae in Markazi Province, Iran

Emergence of CTX-M-15 type extended-spectrum β-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates

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