Chromosome diversity and karyotype asymmetry analysis in four cultivated triploid and three diploid wild genotypes of Musa from North-East India

Dehery, Subrat Kumar; Panda, Eleena; Saha, Priya; Sinha, Rabindra Kumar; Das, Anath Bandhu

doi:10.1007/s13237-020-00334-z

Cited by 12 publications

(4 citation statements)

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“…But cv. Amritapani differs from others with the presence of more number of secondary constriction and the karyotype is comparatively more fragile and karyotype asymmetry analysis might through some light on karyotype evolution in banana (Dehery et al 2020).…”

Section: Discussionmentioning

confidence: 82%

“…The dissymmetrical coefficient of the karyotype through FISH in Hibiscus mutabilis f. mutabilis, L. confirms relatively advanced type over plants with symmetrical chromosomes of the primitive type with respect to evolution (Li et al 2015). Duplication of chromosomes or translocation between the chromosomes with or without secondary constrictions at a very early stage of evolution might be the reason for the structural alteration of the chromosome morphology as well as the variation of secondary constricted chromosomes in the above cultivars (Das and Das 1994;Rai et al 1997;Ghosh et al 2013;Das et al 2015Das et al , 2020Dehery et al 2020).…”

Section: Discussionmentioning

confidence: 92%

“…Edible bananas have 2n = 2x 22, 33 or 44 chromosomes for diploid, triploid and tetraploid cultivars respectively (Stover and Simmonds 1987). These cultivars have a wide range of genome permuta- (Ghosh et al 2013;Das et al 2020;Dehery et al 2020) and molecular marker analysis (Venkatachalam et al 2008), though there are many biodiversity hotspots of banana in North East India exists which need to be explored. Banana varieties of Odisha have remarkable popularity in the locality and the cytogenetics of some of the varieties like cv.…”

Section: Discussionmentioning

confidence: 99%

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Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

Dehury¹,

Dehery

Das

2021

Caryologia

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Banana (Musa spp.) cultivars especially dessert banana are important cash crop with high market demand all over the world as an integral part of the diet. The need for assessment of cytogenetic characters in Musa cultivars is inevitable as out of thousands of cultivars, cytogenetic characterization of most of them remains unresolved due to difficulties like small chromosome size, diversity in ploidy levels and high cultivar diversity which behave differently to standardized cytogenetic protocols. In this report, somatic chromosome number, detailed karyotype analysis including total chromosome length, volume, form percentage, Interphase Nuclear Volume (INV) were accessed on eight dessert type of Musa accessions from different places of Odisha. All the cultivars studied were found triploid (2n = 33) with a basic chromosome number of x=11. The karyotype formulae were assigned to each cultivar by grouping the chromosome according to their shared characteristics. The total chromosome length ranged from 54.95 µm in cv. Robusta to 81.5 µm in cv. Kathia with symmetric karyotype in all the studied cultivar. Karyotype formula revealed structural alteration of chromosome with Total Form percentage (TF%) variation from 35.65% in cv. Amritapani to 41.68% in cv. Patakpura that confirms more number of nearly median constricted chromosome as compared to sub-median chromosome. The total chromosome volume recorded from 10.78 µm3 in cv. Robusta to 15.99 µm3 in cv. Khatia and the INV varied from 1336.44 µm3 in cv. Dwarf Cavendish to 2048.37 µm3 in cv. Patakpura. The recorded structural variation might be due to differential genome specific condensation of chromosome. Chromosome length and volume found statistically significant among the cultivars.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

Dehury¹,

Dehery

Das

2021

Caryologia

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“…Although chromosome counting is accurate and applied primarily to plants with large chromosomes, challenges remain with regard to the banana's chromosome; due to its small, indistinguishable size and high level of metaphase condensation, it is hard to obtain a high quality of chromosome spread [27,28]. There is limited information concerning the structural characteristics of chromosomes and karyotypes for the Musa species, and detailed information about the chromosomes' number and their size, shape, and arrangement within this genus remains scarce [29]. A variety of indirect methods for determining banana ploidy level have been reported [30,31].…”

Section: Chromosome Number and Ploidy Statusmentioning

confidence: 99%

A Flow Cytometry-Based Assessment of the Genomic Size and Ploidy Level of Wild Musa Species in India

Natarajan,

Pathania,

Singh

et al. 2023

Plants

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The genome size variation is an important attribute in evolutionary and species characterization. Musa L. is regarded as one of the taxonomically complicated genera within the order Zingiberales, with more than 75 species from wild seeded to seedless cultivars that may be diploid, triploid or tetraploid. The knowledge of total nuclear DNA content in terms of genome size and ploidy level in wild species of Musa is absolutely important in evolutionary and genomic studies. Methods: In this paper, chromosome spreading was performed via protoplast isolation and a fast air-dry dropping method and flow cytometry were used with Raphanus sativus L. (Brassicaceae) as a standard for ploidy and genome size estimation. Results: The results showed that genome size (2C) varied amongst Musa species, based on the ratio of G1 peak positions. The lowest genome size (2C) was found in M. balbisiana var. andamanica (1.051 ± 0.060 pg) and the highest genome size (2C) was recorded for Musa ABB.cv. Meitei-hei (1.812 ± 0.108 pg) for the section Eumusa. Among the species belonging to the section Rhodochlamys, M. rosae had the lowest 2C content of 1.194 ± 0.033 pg whereas the highest nuclear DNA content (2C) was observed in M. velutina (1.488 ± 0.203 pg). Cytogenetic analysis revealed that the chromosome number of 14 wild Musa species was 2n = 22, while 1 species—Ensete glaucum—showed a chromosome number of 2n = 18 (diploid), and for 3 species, the chromosome number was 2n = 33 (triploids). An association study based on the Pearson correlation coefficient showed 2C nuclear DNA content was significant and positively correlated with ploidy level (R = 0.9) and chromosome number (R = 0.84). Conclusions: The present study provides reliable information on the genome size and ploidy level of wild Musa species from the Indian region through flow cytometric analysis, which could be further utilized in taxonomic and crop improvement programs. For the first time, the nuclear DNA content of eight wild diploid and three triploid Indian species were estimated and reported. Genome size could be an effective indicator in identification of species and evolutionary studies in Musa with varying ploidy levels and morphological similarities.

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Improvement of Nutraceutical Traits of Banana: New Breeding Techniques

Tripathi,

Ntui,

Malarvizhi

et al. 2023

Compendium of Crop Genome Designing for Nutraceuticals

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Chromosome diversity and karyotype asymmetry analysis in four cultivated triploid and three diploid wild genotypes of Musa from North-East India

Cited by 12 publications

References 40 publications

Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

A Flow Cytometry-Based Assessment of the Genomic Size and Ploidy Level of Wild Musa Species in India

Improvement of Nutraceutical Traits of Banana: New Breeding Techniques

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