Chromosome differentiation and genome organization in carnivorous plant family Droseraceae

Shirakawa, Junichi; Hoshi, Yoshikazu; Kondo, Katsuhiko

doi:10.3199/iscb.6.111

Cited by 10 publications

(11 citation statements)

References 49 publications

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“…A similar value of 1.76 pg was obtained from flow cytometer measuring data by Shirakawa et al (2011). On the other hand, Greilhuber (2008) and Tungkajiwangkoon et al (2016) determined a 2C-value of 2.73 pg for this species, which is the same as our present data.…”

Section: Discussionsupporting

confidence: 91%

“…Because our estimated 2C data of D. rotundifolia with the rice reference standard was the same as that of Greilhuber (2008), we believe that present C-values of all plant strains in the Droseraceae are also reliable. The first C-value records of two monotypic genera, Aldrovanda and Dionaea were given by Shirakawa et al (2011). Our 2C-value of A. vesiculosa (2C=1.2 pg) is similar to that of the previous report (2C=1.0 pg).…”

Section: Discussionsupporting

confidence: 87%

“…C-values of 11 species in the Droseraceae have previously been recorded (Rothfels and Heimburger 1968, Greilhuber 2008, Shirakawa et al 2011, Veselý et al 2012, Jensen et al 2015, Tungkajiwangkoon et al 2016. In this study, C-value data of D. anglica, D. burmannii and D. communis were measured for the first time.…”

Section: Discussionmentioning

confidence: 89%

“…Rosenberg 1903Rosenberg , 1904Rosenberg , 1909, little work on nuclear DNA content measurements for genome size determination has focused specifically on this group. For example, only a few pa-pers have dealt with C-value estimations of Drosera species, and with one exception (Rothfels and Heimburger 1968), these have all been published since 2000 (Greilhuber 2008, Shirakawa et al 2011, Veselý et al 2012, Záhumenická et al 2013. It is indeed over 30 years since the first Drosera genome size was recorded.…”

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confidence: 99%

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Determination of Ploidy Level and Nuclear DNA Content in the Droseraceae by Flow Cytometry

et al. 2017

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2C-values of nine species of the genus Drosera, and two monotypic genera Aldrovanda and Dionaea were estimated to provide an overview of the genome diversity and chromosome differentiation in the Droseraceae. The measured DNA contents of all species used in this study ranged over nine-fold from 2C=0.63 pg in D. burmannii to 5.67 pg in D. anglica. In the genus Drosera, even though the polyploid species were excluded, the difference of the 2C DNA contents among diploid species was still high, ranging 4.3-fold from 0.66 pg in D. spatulata to 2.85 pg in D. intermedia. In subgenus Drosera, especially the polyploidal group, two chromosome types were identified by means of their size; this therefore made it possible to discriminate two groups of the genomes: one group was of a smaller genome size (S genome group) consisting of a total of 10 small-sized chromosomes (x=10s), and the other group was of a larger genome size (M genome group) consisting of a total of 10 middle-sized chromosomes (x=10 m). The Cx-value of the S genome group was less than 0.4 pg (ca. 400 Mbp). On the other hand, the Cx-value of the M genome group showed a range of 1.3-1.5 pg (1270-1470 Mbp). Moreover, the 2C DNA content of the hexaploid species D. tokaiensis (2n=6x=20 m+40s, 2C= 3.57 pg), which originated from naturally occurring interspecific hybridization event between D. rotundifolia (2n=2x=20 m, 2C=2.73 pg) and the tetraploid D. spatulata (2n=4x=40s, 2C=1.38 pg), was less (86.9%) than the sum of their putative parental species.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 99%

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Determination of Ploidy Level and Nuclear DNA Content in the Droseraceae by Flow Cytometry

et al. 2017

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“…However, in D. rotundifolia, chromosomal fragments induced by gamma irradiation are regularly inherited by daughter cells during mitosis (Shirakawa et al, 2011a) which is strong evidence for chromosomal holokinetism; D. aliciae and D. binata have not been studied this way. It is therefore possible that H2AThr120ph is not a universal mitotic centromere marker or at least not able definitely to distinguish between monocentric and holokinetic chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Evolution of genome size and genomic GC content in carnivorous holokinetics (Droseraceae)

Veleba

Šmarda

Zedek

et al. 2016

Ann Bot

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Background and Aims Studies in the carnivorous family Lentibulariaceae in the last years resulted in the discovery of the smallest plant genomes and an unusual pattern of genomic GC content evolution. However, scarcity of genomic data in other carnivorous clades still prevents a generalization of the observed patterns. Here the aim was to fill this gap by mapping genome evolution in the second largest carnivorous family, Droseraceae, where this evolution may be affected by chromosomal holokinetism in Drosera.Methods The genome size and genomic GC content of 71 Droseraceae species were measured by flow cytometry. A dated phylogeny was constructed, and the evolution of both genomic parameters and their relationship to species climatic niches were tested using phylogeny-based statistics.Key Results The 2C genome size of Droseraceae varied between 488 and 10 927 Mbp, and the GC content ranged between 37Á1 and 44Á7 %. The genome sizes and genomic GC content of carnivorous and holocentric species did not differ from those of their non-carnivorous and monocentric relatives. The genomic GC content positively correlated with genome size and annual temperature fluctuations. The genome size and chromosome numbers were inversely correlated in the Australian clade of Drosera.Conclusions Our results indicate that neither carnivory (nutrient scarcity) nor the holokinetism have a prominent effect on size and DNA base composition of Droseraceae genomes. However, the holokinetic drive seems to affect karyotype evolution in one of the major clades of Drosera. Our survey confirmed that the evolution of GC content is tightly connected with the evolution of genome size and also with environmental conditions.

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Holocentric chromosomes may be an apomorphy of Droseraceae

et al. 2018

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Chromosome differentiation and genome organization in carnivorous plant family Droseraceae

Cited by 10 publications

References 49 publications

Determination of Ploidy Level and Nuclear DNA Content in the Droseraceae by Flow Cytometry

Determination of Ploidy Level and Nuclear DNA Content in the Droseraceae by Flow Cytometry

Evolution of genome size and genomic GC content in carnivorous holokinetics (Droseraceae)

Holocentric chromosomes may be an apomorphy of Droseraceae

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