Chromosome breakage, fusion and reconstruction during P1dl transduction

Rae, M. Engel; Stodolsky, Marvin

doi:10.1016/0042-6822(74)90139-1

Cited by 21 publications

(15 citation statements)

References 22 publications

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“…They thus differ from the particles of P22 tetR and PI dl phages described by Chan el a/. ( I 972) and Rae & Stodolsky (1974), respectively, which must be complemented by another particle to achieve expression.…”

Section: Discussionmentioning

confidence: 99%

“…The rapid fall of the curve for HFT alone (solid circles) between m.0.i. of I and I O -~ (where chances of simultaneous infection by transducing and normal particles present in the lysate are slight) is not indicative of mutual aid between particles ; see also Rae & Stodolsky, 1974). The presence of helper phage, at a m.0.i.…”

Section: Defectiveness Of Hft Phagementioning

confidence: 99%

“…In addition, the Providence HFT phage was not capable of general transduction. A possible explanation is that a permutation of the excision event (Luria et al 1960;Matsushiro, 1963;Rae & Stodolsky, 1974; see also Weigle, Meselson & Paigen, 1959;Adler & Templeton, 1963) of the prophage occurred in individual bacteria of a lysate and this resulted in individual vegetative phage genomes containing complete or defective portions of the kanamycinresistance gene. Watanabe et al (1972) found that nine out of ten transductants obtained from a ~2 2 HFT lysate for tetracycline resistance produced HFT lysates on U.V.…”

Section: Efect Of Uv Irradiation Of Hft Lysates On Transductionmentioning

confidence: 99%

“…Specialized transducing phages vary in the complement of phage functions retained. There are the tetR P22 and Pzdl (Rae & Stodolsky, 1974) phages which cannot multiply or lysogenize on single infection, the variants of phage epsilon (Kameda et al 1965) and P22 (Hoppe & Roth, 1974) which can lysogenize but exhibit no immunity or maturation functions, and the semi-defective particles of hdg and P22 which can lysogenize and retain immunity properties but possess no (Campbell, I 957 ; Smith-Keary, I 966) or virtually no (Dubnau & Stocker, 1964) maturation function. Finally, there are converting phages PICM (Kondo & Mitsuhashi, 1964) and q5 Amp+ (Williams Smith, 1972) which retain all phage functions.…”

Section: Efect Of Uv Irradiation Of Hft Lysates On Transductionmentioning

confidence: 99%

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Specialized Transduction of Kanamycin Resistance in a Providence Strain

Coetzee

1975

Journal of General Microbiology

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Properties of a transducing system with a phage able to transduce a kanamycinresistance marker of the T compatibility group plasmid R394 at a frequency of 2 x Io-2/plaque-forming unit adsorbed are described. The phage was detected in Providence strain ~2 9 transduced to kanamycin resistance by Providence phage PL25 grown on this strain harbouring the R factor. Four ~2 9 transductants, specially selected at the lowest multiplicities of infection of the high frequency transducing (HFT) phage, were defective lysogens. They plated PL25 with an efficiency of I and only one liberated low-titre phage spontaneously or on U.V. induction. The defect in maturation function could be corrected by introduction of a wild PL25 prophage. The transducing phage was serologically identical to PL25. It could transduce in single infection, but transduction frequency was increased by the simultaneous presence of homologous non-transducing phage. Transductants did not transfer the kanamycin-resistance marker by conjugation, and produced kanamycin-sensitive segregants at a moderate rate. These segregants could be transduced to kanamycin resistance by the HFT phage. Irradiation of HFT lysates by U.V. produced an exponential fall in transduction frequency. It was concluded that the defective phage transduced by lysogenization. Kanamycinresistant transductants could themselves be transduced to streptomycin resistance by PL25 reared on a streptomycin-resistant mutant. Lysogenic transductants produced by the HFT phage did not always liberate HFT phage on U.V. induction. Possible explanations are considered. I N T R O D U C T I O NTransduction of a Proteus rettgeri R factor R394 (Coetzee, Datta & Hedges, 1972) by vector phage 34 (Coetzee & Sacks, 1960) to Proteus mirabilis strain PM5006 yielded some transductants which only registered the kanamycin-resistance marker of the plasmid and could not transmit the marker by conjugation. The latter transductants were lysogenic and liberated phage able to transduce kanamycin resistance to ~~5 0 0 6 at a frequency of 3 x I o -~/ plaque-forming unit (p.f.u.) adsorbed (Coetzee, I 974 b). Transfer of R394 to these transductants yielded some progeny which, on U.V. induction, produced lysates that could transduce markers of kanamycin and ampicillin resistance at frequencies of 4 x Io-2/p.f.u. adsorbed (Coetzee, 1975). Apart from the additional marker of the latter phage, properties of the two high frequency transducing (HFT) phages were similar. They were serologically identical to phage 34 and could transduce at a multiplicity of infection (m.0.i.) of less than 0-01. Transduction frequencies were increased about tenfold by the presence of homologous non-transducing phage. HFT lysates could also transduce various chromosomal markers at low frequencies. Transductants produced kanamycin-sensitive lysogenic segregants at high rates. Strain ~~5 0 0 6 is cryptically lysogenic (Krizsanovich, 1973) for a phage

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Defectiveness Of Hft Phagementioning

confidence: 99%

Section: Efect Of Uv Irradiation Of Hft Lysates On Transductionmentioning

confidence: 99%

Section: Efect Of Uv Irradiation Of Hft Lysates On Transductionmentioning

confidence: 99%

See 2 more Smart Citations

Specialized Transduction of Kanamycin Resistance in a Providence Strain

Coetzee

1975

Journal of General Microbiology

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“…In the lysogenic state, bacteriophage P1 replicates as an autonomous plasmid [1]. Although the P1 prophage does not integrate into the host chromosome, several specialized transducing derivatives of P1 carrying chromosomal markers such as arg, lac or pro have been isolated [2][3][4][5]. The Pllac derivatives isolated so far are oversized, i.e.…”

Section: Introductionmentioning

confidence: 99%