Chromosome behavior during meiosis in pollen mother cells from Saccharum officinarum × Erianthus arundinaceus F1 hybrids

Abstract

Background In recent years, sugarcane has attracted increasing attention as an energy crop. Wild resources are widely used to improve the narrow genetic base of sugarcane. However, the infertility of F1 hybrids between Saccharum officinarum (S. officinarum) and Erianthus arundinaceus (E. arundinaceus) has hindered sugarcane breeding efforts. To discover the cause of this infertility, we studied the hybridization process from a cytological perspective. Results We examined the meiotic process of pollen mother cells (PMCs) in three F1 hybrids between S. officinarum and E. arundinaceus. Cytological analysis showed that the male parents, Hainan 92–77 and Hainan 92–105, had normal meiosis. However, the meiosis process in F1 hybrids showed various abnormal phenomena, including lagging chromosomes, micronuclei, uneven segregation, chromosome bridges, and inability to form cell plates. Genomic in situ hybridization (GISH) showed unequal chromatin distribution during cell division. Interestingly, 96.70% of lagging chromosomes were from E. arundinaceus. Furthermore, fluorescence in situ hybridization (FISH) was performed using 45S rDNA and 5S rDNA as probes. Either 45S rDNA or 5S rDNA sites were lost during abnormal meiosis, and results of unequal chromosomal separation were also clearly observed in tetrads. Conclusions Using cytogenetic analysis, a large number of meiotic abnormalities were observed in F1. GISH further confirmed that 96.70% of the lagging chromosomes were from E. arundinaceus. Chromosome loss was found by further investigation of repeat sequences. Our findings provide insight into sugarcane chromosome inheritance to aid innovation and utilization in sugarcane germplasm resources.

Cytomixis and aberrant phenomena during meiosis in pollen mother cells ofCamellia sinensisvar.sinensiscv ‘Fudingdabai’

“…Micronucleus formation was caused by chromosome lag that prevented chromosomes from entering newly formed cells [27] . The formation of lagging chromosomes is a product of several pre-anaphasic chromosomal irregularities [28] .…”

Crossing-over decision landscape in maize

“…The copyright holder for this preprint this version posted September 22, 2022. ; https://doi.org/10.1101/2022.09.21.508771 doi: bioRxiv preprint species in which creating mapping populations is challenging and in inter-specific hybrids, where mature COs may lead to chromosome instabilities, preventing conventional mapping (Li et al, 2021;Morales and Dujon, 2012).…”

“…Using this approach, we found that a large proportion of COs in maize are formed in SNP desert regions, where conventional methods are unable to determine precise CO locations. MLH3 ChIP should also be useful for genetic mapping in species in which creating mapping populations is challenging and in inter-specific hybrids, where mature COs may lead to chromosome instabilities, preventing conventional mapping (Li et al, 2021; Morales and Dujon, 2012).…”

Variation of Chromosome Composition in a Full-Sib Population Derived From 2x × 3x Interploidy Cross of Populus

“… Zhao et al (2019) found dramatically biased pairing of homoeologous chromosomes and different univalent and chromosome lagging frequencies between C- and H-subgenomes in interspecific hybrids and allotetraploid Cucumis × hytivus through oligo-painting and genomic in situ hybridization (GISH), concluding that the meiotic behavior harmony of subgenomes is important for meiotic evolution. In Saccharum officinarum × Erianthus arundinaceus hybrids, unequal chromosome segregation and chromosome losing were observed during meiosis based on 5S rDNA and 45S rDNA FISH analyses ( Li et al, 2021 ). Definitely, the FISH analysis has developed into a reliable tool for tracing the chromosome behavior during meiosis of plants.…”