Chromosome Banding: Stain Combinations for Specific Regions

Schweizer, Dieter; Ambros, Peter F.

doi:10.1385/0-89603-289-2:97

Cited by 77 publications

(51 citation statements)

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“…Probe detection was performed using tetramethylrhodamine isothiocyanate-labeled antibodies. To further define the regional localization, chromosome banding was performed (21).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Exon in Apolipoprotein E Receptor 2 Leading to Alternatively Spliced mRNAs Found in Cells of the Vascular Wall but Not in Neuronal Tissue

Korschineck¹,

Ziegler²,

Breuss³

et al. 2001

Journal of Biological Chemistry

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“…Probe detection was performed using tetramethylrhodamine isothiocyanate-labeled antibodies. To further define the regional localization, chromosome banding was performed (21).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Exon in Apolipoprotein E Receptor 2 Leading to Alternatively Spliced mRNAs Found in Cells of the Vascular Wall but Not in Neuronal Tissue

Korschineck¹,

Ziegler²,

Breuss³

et al. 2001

Journal of Biological Chemistry

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“…To reveal the GC-or AT-rich chromosome sites, root tip preparations were stained with 4'-6-diamidino-2-phenyloindole (DAPI) or chromomycin A 3 (CMA 3 ) and respectively counterstained with actinomycin D (AMD) or distamycin A (DA), as described by Schweizer and Ambros [15]. After the washing and drying, the preparations were sealed with rubber cement (Marabu).…”

Section: Base-specific Fluorescencementioning

confidence: 99%

Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea

Golczyk

Hasterok

Szklarczyk

2010

Cellular and Molecular Biology Letters

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High-and low-stringency FISH and base-specific fluorescence were performed on the permanent translocation heterozygote Rhoeo spathacea (2n = 12).Our results indicate that 45S rDNA arrays, rDNA-related sequences and other GC-rich DNA fraction(s) are located within the pericentromeric regions of all twelve chromosomes, usually colocalizing with the chromomycin A 3 -positive bands. Homogenization of the pericentromeric regions appears to result from the concerted spread of GC-rich sequences, with differential amplification likely. We found new 5S rDNA patterns, which suggest a variability in the breakpoints and in the consequent chromosome reorganizations. It was found that the large 5S rDNA locus residing on each of the 8E and 9E arms consisted of two smaller loci. On each of the two chromosome arms 3b and 4b, in addition to the major subtelomeric 5S rDNA locus, a new minor locus was found interstitially about 40% along the arm length. The arrangement of cytotogenetic landmarks and chromosome arm measurements are discussed with regard to genome repatterning in Rhoeo.

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“…Fluorescence in situ hybridization (FISH) on DAPI banded metaphase chromosomes (Schweizer and Ambros, 1994) from two normal, healthy donors (male and female) was performed by a modi®cation of the protocols of (Lichter et al, 1990). Cells were harvested and slides prepared according to standard cytogenetic techniques.…”

Section: In Situ Hybridizationmentioning

confidence: 99%