Chromosome assignment of the gene loci ETS1 and THY1 in the owl monkey

Abstract

Our previous assignment of the gene loci HBB, HRASl, INS, PTH, LDHA, and CAT to owl monkey chromosome 19 of karyotype VI (K-VI) indicated a putative homology of this owl monkey chromosome with the short arm of human chromosome 11 (HSA 11p). To investigate further the extent of shared homolgy, we localized in the owl monkey complement two genes known to be on HSA 11q. Segregation analysis of ETS1 and THY1 homologous DNA in three karyotypically different panels of rodent × owl monkey somatic cell hybrids provided evidence for the syntenic assignment of these loci to homologous chromosomes of three owl monkey karyotypes, namely, chromosomes 4 (K-VI), 3 (K-II), and 5 (K-V). The results indicate a disruption of syntenic gene loci on the distal portion of HSA 11q from 11p during primate evolution.

Gene Maps of Nonhuman Primates

“…The panel members are then typed for presence or absence of particular genes or gene products, and chromosomal location is inferred from the concordances and discordances with the presence of each primate chromosome. This approach has been used to map genes to particular chromosomes in numerous primate species (for example, Ma and Gerhard 1988;Ma and Kurnit 1984;Thiessen and Lalley 1987). Since the early 1990s, FISH has become the most commonly employed approach.…”