Chromosome Architecture and Gene Content of the Emergent Pathogen Acinetobacter haemolyticus

Castro-Jaimes, Semiramis; Bello-López, Elena; Velázquez-Acosta, Consuelo; Volkow-Fernández, Patricia; Lozano-Zaraín, Patricia; Castillo-Ramírez, Santiago; Cevallos, Miguel A.

doi:10.3389/fmicb.2020.00926

Cited by 5 publications

(5 citation statements)

References 114 publications

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“…We describe a group of 119 plasmids spanning A. baumannii strains from thirteen sequence types, and non- A. baumannii strains including A. seifertii , A. haemolyticus, and Klebsiella pneumoniae . A. seifertii and A. haemolyticus are also considered to be emerging pathogens of clinical importance [ 61 , 62 ], and carbapenem-resistant and/or MDR strains have been identified both clinically and environmentally [ 15 , 61 , 63–66 ]. A. seifertii is associated with serious human infections including bloodstream infections [ 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the diversity of type IV secretion system-encoding plasmids in Acinetobacter

Nasser,

Gaudreau,

Lubega

et al. 2024

Emerging Microbes & Infections

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The multi-drug resistant pathogen Acinetobacter baumannii has gained global attention as an important clinical challenge. Owing to its ability to survive on surfaces, its capacity for horizontal gene transfer, and its resistance to front-line antibiotics, A. baumannii has established itself as a successful pathogen. Bacterial conjugation is a central mechanism for pathogen evolution. The epidemic multidrug-resistant A. baumannii ACICU harbours a plasmid encoding a Type IV Secretion System (T4SS) with homology to the E. coli F-plasmid, and plasmids with homologous gene clusters have been identified in several A. baumannii sequence types. However the genetic and host strain diversity, global distribution, and functional ability of this group of plasmids is not fully understood. Using systematic analysis, we show that pACICU2 belongs to a group of almost 120 T4SS-encoding plasmids within four different species of Acinetobacter and one strain of Klebsiella pneumoniae from human and environmental origin, and globally distributed across 20 countries spanning 4 continents. Genetic diversity was observed both outside and within the T4SS-encoding cluster, and 47% of plasmids harboured resistance determinants, with two plasmids harbouring eleven. Conjugation studies with an extensively drug-resistant (XDR) strain showed that the XDR plasmid could be successfully transferred to a more divergent A. baumanii , and transconjugants exhibited the resistance phenotype of the plasmid. Collectively, this demonstrates that these T4SS-encoding plasmids are globally distributed and more widespread among Acinetobacter than previously thought, and that they represent an important potential reservoir for future clinical concern.

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Section: Discussionmentioning

confidence: 99%

Characterization of the diversity of type IV secretion system-encoding plasmids in Acinetobacter

Nasser,

Gaudreau,

Lubega

et al. 2024

Emerging Microbes & Infections

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“…The genomic DNA of A. haemolyticus AN54 was used as a PCR template to amplify different regions of one of its plasmids, pAhaeAN54e. The description and genome sequence of this strain have been published previously [15,16]. To test the replication capabilities of the constructs, a spontaneously pAhaeAN54e-cured strain resistant to rifampicin was used as a recipient for all the constructs.…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…The pAhaeAN54e plasmid (NZ_CP041229.1), a member of the pNDM-YR7 clade, is a medium-size replicon that was identified in the Acinetobacter haemolyticus strain AN54, isolated in a Mexican hospital. This plasmid contains an NDM-1 gene linked to carbapenem resistance and an aphA6 gene involved in aminoglycoside resistance, and both genes lie close to an ISAba125 element [15,16]. This plasmid also encodes proteins involved in maintenance and transfer and 19 proteins with unknown functions.…”

Section: Introductionmentioning

confidence: 99%

A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene

Bello-López,

Pérez-Oseguera,

Santos

et al. 2024

PLoS ONE

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The blaNDM-1 gene and its variants encode metallo-beta-lactamases that confer resistance to almost all beta-lactam antibiotics. Genes encoding blaNDM-1 and its variants can be found in several Acinetobacter species, and they are usually linked to two different plasmid clades. The plasmids in one of these clades contain a gene encoding a Rep protein of the Rep_3 superfamily. The other clade consists of medium-sized plasmids in which the gene (s) involved in plasmid replication initiation (rep)have not yet been identified. In the present study, we identified the minimal replication region of a blaNDM-1-carrying plasmid of Acinetobacter haemolyticus AN54 (pAhaeAN54e), a member of this second clade. This region of 834 paired bases encodes three small peptides, all of which have roles in plasmid maintenance. The plasmids containing this minimal replication region are closely related; almost all contain blaNDM genes, and they are found in multiple Acinetobacter species, including A. baumannii. None of these plasmids contain an annotated Rep gene, suggesting that their replication relies on the minimal replication region that they share with the plasmid pAhaeAN54e. These observations suggest that this plasmid lineage plays a crucial role in the dissemination of the blaNDM-1 gene and its variants.

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“…Some outstanding species, such as A. hemolyticus, were found in the general environment and tended to cause severe clinical cases concerned with the environment in hospitals. On the other hand, AMR, particularly carbapenems, was not determined clearly [18]. A. junii has been reported in calculi obstructions in the ureter [19].…”

Section: Introductionmentioning

confidence: 99%

Prevalence, species, and antimicrobial resistance of Acinetobacter in surgical practice and laboratory dog husbandry room environments

et al. 2021

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Acinetobacter is a bacteria found in the environment and clinical specimens, causing nosocomial infection and antimicrobial resistance (AMR) threats. This study examined the prevalence, species, and AMR characteristics of Acinetobacter isolated from surgical practice and the laboratory dog husbandry room environments (n = 235) at Rajamangala University of Technology Tawan-ok veterinary hospital during 2018-2019. The prevalence of Acinetobacter in the laboratory dog husbandry room and veterinary belongings were 2.55% and 0.43%, respectively. Species determination was Acinetobacter hemolyticus (2.1%) and Acinetobacter baumannii (0.4%) from environments in the laboratory dog husbandry room, and Acinetobacter junii (0.4%) from the shoes used in the surgical practice room. AMR was observed in both study environments and the specimens sent to the Veterinary Diagnostic Center. These isolates had a high resistant percentage to amoxicillin-clavulanic acid (84.62%), sulfamethoxazole-trimethoprim (61.54%), and cephalexin (53.85%) but were susceptible to imipenem. Compared to the isolates recovered from the clinical specimens, most isolates derived from environments exhibited multidrug resistance and shared correlated resistance patterns. These results highlight the need for sanitization in the dog husbandry room. Furthermore, the AMR results can be used as a preliminary baseline for studying AMR Acinetobacter contamination in animals and their environments.

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Chromosome Architecture and Gene Content of the Emergent Pathogen Acinetobacter haemolyticus

Cited by 5 publications

References 114 publications

Characterization of the diversity of type IV secretion system-encoding plasmids in Acinetobacter

Characterization of the diversity of type IV secretion system-encoding plasmids in Acinetobacter

A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene

Prevalence, species, and antimicrobial resistance of Acinetobacter in surgical practice and laboratory dog husbandry room environments

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