Chromosome and karyotype analysis of <i>Hibiscus mutabilis</i> f. <i>mutabilis</i>

Li, Yuping; Zhang, Xinlei; Wu, Wenting; Miao, Shu-xing; Chang, Junli

doi:10.1080/21553769.2015.1041166

Cited by 17 publications

(8 citation statements)

References 7 publications

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“…Compared with other traits, chromosomal traits are always retained in plants [5], and chromosome number and morphology are important components of karyotype [6]. Karyotypes are used to reveal genome organization at the chromosomal level [7], reveal cytological characteristics [8], and provide information on species origin, phylogeny, genetics and breeding, and variety improvement [9,10], and can provide a basis for plant naming [11]. Understanding the development of karyotypes helps to better understand the organization and evolution of chromosomes in related species [12].…”

Section: Introductionmentioning

confidence: 99%

Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Zhang

Luo

et al. 2022

Genes

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Most Fabaceae have nitrogen fixation abilities and are valuable forage and medicinal resources. However, cytogenetic data of many Fabaceae species are unclear. Karyotypes reveal cytological characteristics and are crucial to understanding the organization and evolution of chromosomes in species. Oligo-FISH can reveal genetic composition and karyotype variation patterns with rapid and efficient results. Karyotype analysis of five Fabaceae species by oligonucleotide probes showed that: Robinia pseudoacacia, karyotype formula 2n = 2x = 20m + 2sm, cytotype 2B, arm ratio 3.4821, eight chromosomes distributed 5S rDNA signal. The karyotype formula of Robinia pseudoacacia ‘idaho’ was 2n = 2x = 20m + 2sm, cytotype 1A, arm ratio 1.8997, and 5S rDNA signal was distributed on six chromosomes. Karyotype of Robinia pseudoacacia f. decaisneana 2n = 2x = 20m + 2sm, cytotype 1B, arm ratio 2.0787, the distribution of eight chromosomes with 5S rDNA signal. Karyotype formula of Styphnolobium japonicum 2n = 2x = 14m + 12sm + 2st, cytotype 2B, arm ratio 2.6847, two chromosomes have 5S rDNA signal. Amorpha fruticose karyotype 2n = 2x = 38m + 2sm, cytotype 1B, arm ratio 3.2058, four chromosomes possessed 5S rDNA signal. Both ends of all species’ chromosomes have (AG3T3)3 signals. The results of this study provide chromosome numbers and a physical map, contributing to the construction of the Oligo-FISH barcode and providing molecular cytogenetics data for Fabaceae.

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Section: Introductionmentioning

confidence: 99%

Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Zhang

Luo

et al. 2022

Genes

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“…Furthermore, translocation mediated structural alteration played a crucial role in chromosome evolution (Lysak et al 2006;Luiz et al 2009) besides heteromorphicity in centromeriac position among the chromosomes of Allium localizing GC-and AT-rich repeats by CMA-and DAPI-banding patterns (Mahbub et al 2014). The dissymmetrical coefficient of the karyotype through FISH in Hibiscus mutabilis f. mutabilis, L. confirms relatively advanced type over plants with symmetrical chromosomes of the primitive type with respect to evolution (Li et al 2015). Duplication of chromosomes or translocation between the chromosomes with or without secondary constrictions at a very early stage of evolution might be the reason for the structural alteration of the chromosome morphology as well as the variation of secondary constricted chromosomes in the above cultivars (Das and Das 1994;Rai et al 1997;Ghosh et al 2013;Das et al 2015Das et al , 2020Dehery et al 2020).…”

Section: Discussionmentioning

confidence: 96%

Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

Dehury¹,

Dehery

Das

2021

Caryologia

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Banana (Musa spp.) cultivars especially dessert banana are important cash crop with high market demand all over the world as an integral part of the diet. The need for assessment of cytogenetic characters in Musa cultivars is inevitable as out of thousands of cultivars, cytogenetic characterization of most of them remains unresolved due to difficulties like small chromosome size, diversity in ploidy levels and high cultivar diversity which behave differently to standardized cytogenetic protocols. In this report, somatic chromosome number, detailed karyotype analysis including total chromosome length, volume, form percentage, Interphase Nuclear Volume (INV) were accessed on eight dessert type of Musa accessions from different places of Odisha. All the cultivars studied were found triploid (2n = 33) with a basic chromosome number of x=11. The karyotype formulae were assigned to each cultivar by grouping the chromosome according to their shared characteristics. The total chromosome length ranged from 54.95 µm in cv. Robusta to 81.5 µm in cv. Kathia with symmetric karyotype in all the studied cultivar. Karyotype formula revealed structural alteration of chromosome with Total Form percentage (TF%) variation from 35.65% in cv. Amritapani to 41.68% in cv. Patakpura that confirms more number of nearly median constricted chromosome as compared to sub-median chromosome. The total chromosome volume recorded from 10.78 µm3 in cv. Robusta to 15.99 µm3 in cv. Khatia and the INV varied from 1336.44 µm3 in cv. Dwarf Cavendish to 2048.37 µm3 in cv. Patakpura. The recorded structural variation might be due to differential genome specific condensation of chromosome. Chromosome length and volume found statistically significant among the cultivars.

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“…Chromosome data are useful for species classification and the basis of cytogenetics. Through karyotyping between Bletilla , information about the origin, phylogeny, genetic breeding, and variety improvements in different species can be learned [ 35 , 36 ]. The use of oligonucleotide systems enabled the first comprehensive cytogenetic analysis of Bletilla in the genomics era.…”

Section: Introductionmentioning

confidence: 99%

The Genetic Diversity of Bletilla spp. Based on SLAF-seq and Oligo-FISH

Huan

Lei

et al. 2022

Genes

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Bletilla spp.Rchb. F. is a traditional Chinese medicinal material. In this study, Bletilla striata (Thunb. ex A. Murray) Rchb F, Bletilla formosana (Hayata) Schltr, and Bletilla ochracea Schltr were collected to analyze the genetic diversity of 16 materials using specific site-amplified fragment sequencing (SLAF-seq) and fluorescence in situ hybridization (FISH). The results showed that the phylogenetic tree of the single-nucleotide polymorphism (SNP) data rendering system was correlated with the shape and geographical distribution of the material. The results of the population structural analysis showed that all the materials containing yellow labellum came from the same ancestor. The results of the principal component analysis were able to preliminarily judge the genetic distance and provided a reference for the selection of hybrid parents. The FISH analysis showed that the chromosomes of B. striata were 2n = 32 and the chromosomes of the B. striata (safflower) mutant were 2n = 34 and the chromosomes of B. ochracea and B. formosana were 2n = 34–36. The (AG3T3)3 non-terminal signal was different from the 5S rDNA signal. These results revealed that the 16 materials had rich genetic diversity, which can provide molecular and cytogenetic data for the study of the genus and its relatives and serve as a reference for the breeding of new genus varieties and improve breeding efficiency and cost.

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Chromosome and karyotype analysis of Hibiscus mutabilis f. mutabilis

Cited by 17 publications

References 7 publications

Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Karyotype Variation in Eight Cultivars of Indian Dessert Banana (Musa acuminata L.) of Section Eumusa From Odisha, India

The Genetic Diversity of Bletilla spp. Based on SLAF-seq and Oligo-FISH

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