Chromosome-anchored QTL conferring aluminum tolerance in hexaploid oat

Abstract

Aluminum (Al) toxicity is a major constraint on crop production in acid soils around the world. Hexaploid oat (Avena sativa L.) possesses significant Al tolerance making it a good candidate for production in these environments. Genetic improvement for Al tolerance in oat has traditionally been achieved through conventional plant breeding and could be enhanced by marker-assisted selection. The objectives of this study were to develop a chromosome-anchored genetic map for an oat recombinant inbred population and to identify SNP markers linked to quantitative trait loci (QTL) affecting root growth response to Al. Three QTL on chromosomes 7C-17A, 13A, and 19A conferring Al tolerance were identified using primary root regrowth of recombinant inbred lines derived from the cross between UFRGS 17 (Al tolerant) and UFRGS 930598-6 (Al sensitive). Localization of each QTL onto the sequenced rice genome revealed the genetic region on chromosome 13A might be associated with a putative malate transporter locus (LOC_Os06g15779). Studies of root apex tissue indicated that exudation of malate was increased in the Al-tolerant parent UFRGS17 and not in the sensitive parent. Based on these data, the malate transporter might be a candidate gene responsible for one of the Al tolerance QTL identified in this study.Electronic supplementary material The online version of this article (

Genomics-assisted breeding in minor and pseudo-cereals

“…Studies involving the QTL mapping of other minor and pseudo-cereals, however, are relatively limited and mostly include studies in rye (Erath et al 2016, Miedaner et al 2018, Myśków et al 2018, Wang et al 2015, oat (Admassu-Yimer et al 2019, Babiker et al 2015, Pellizzaro et al 2016, Schneider et al 2015, Sunstrum et al 2018, Zimmer et al 2018, and millet. Of the various types of millet, pearl millet (Ambawat et al 2016, Aparna et al 2015, Kumar et al 2016b, 2018, Pucher et al 2018, Punnuri et al 2016, Taunk et al 2018 and foxtail millet (Fang et al 2016, Mauro-Herrera and Doust 2016, Ni et al 2017, Odonkor et al 2018, Wang et al 2017a, 2017b have received the majority of research attention, although one study also focused on proso millet (Rajput et al 2016).…”

“…Among the strategies used for QTL mapping, the use of single nucleotide polymorphism (SNP) markers is the most common. Such markers can be investigated using either DNA array technologies (e.g., Babiker et al 2015, Dhariwal et al 2018, Erath et al 2017, Schneider et al 2015 or next-generation sequencing (NGS) technologies, such as genotyping-by-sequencing (GBS; Elshire et al 2011) and restriction site-associated DNA sequencing (RAD-Seq; Baird et al 2008, Miller et al 2007, (e.g., Punnuri et al 2016, Rajput et al 2016, Sunstrum et al 2018, Wang et al 2017a, Wen et al 2018. Not a few studies have used simple sequence repeats (SSRs) as markers (e.g., Aparna et al 2015, Fang et al 2016, Taunk et al 2018.…”

“…Studies frequently focused on biotic and abiotic stress resistance, including disease resistance, drought tolerance, frost tolerance, aluminum tolerance, etc. (e.g., Aparna et al 2015, Erath et al 2017, Schneider et al 2015, Sunstrum et al 2018, as well as a variety of general agronomical and morphological traits. QTL mapping has also been performed in minor cereals to identify loci associated with nutrient content (e.g., Kumar et al 2018).…”

Evaluation of aluminum tolerance diversity in Avena sativa L. from the VIR collection

“…С использованием RFLPмаркеров были протестированы регионы, где сравнительное картирование показало вероятность нахождения локусов ортологичных количественных признаков (QTL) толерантности к алюминию у других видов растений (Wright et al, 2006). Для оценки реакции роста корней на воздействие Al разработали хромосомную генетическую карту для рекомбинантной инбредной популяции посевного овса и для идентификации маркеров SNP, связанных с локусами количественных признаков (QTL) (Schneider et al, 2015).…”