Chromosome aberrations (CA), sister-chromatid exchanges (SCE) and mitogen-induced blastogenesis in cultured peripheral lymphocytes from 48 control individuals sampled 8 times over 2 years

Anderson, Diana; Francis, Anne J.; Godbert, P.; Jenkinson, P.C.; Butterworth, K.R.

doi:10.1016/0027-5107(91)90203-z

Cited by 45 publications

(8 citation statements)

References 25 publications

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“…Esses achados estão de acordo com os observados por vários autores (Bender et al, 1988;Galloway et al, 1986;Hedner et al, 1982;Margolin & Shelby, 1985), enquanto que outros autores não encontraram nenhum efeito com relação ao sexo (Anderson et al, 1991).…”

Section: Discussionunclassified

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Pacheco

Hackel

2002

Cad. Saúde Pública

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Section: Discussionunclassified

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Pacheco

Hackel

2002

Cad. Saúde Pública

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“…Similarly, the sex-related differences in mitogeninduced lymphocyte proliferation observed here are in conformity with others. In non-exposed individuals, Anderson et al (1991), observed higher blastogenic transformation in PHA stimulated lymphocytes from women as compared to men. Although Gonsebatt et al 1994 report a greater impairment of lymphocytes in women exposed to arsenic in drinking water as compared to men, they have no measure of dose, which may prove to be higher in women.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon

Amorim

Mergler

Bahia

et al. 2000

An. Acad. Bras. Ciênc.

105

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The mercury rejected in the water system, from mining operations and lixiviation of soils after deforestation, is considered to be the main contributors to the contamination of the ecosystem in the Amazon Basin. The objectives of the present study were to examine cytogenetic functions in peripheral lymphocytes within a population living on the banks of the Tapajós River with respect to methylmercury (MeHg) contamination, using hair mercury as a biological indicator of exposure. Our investigation shows a clear relation between methylmercury contamination and cytogenetic damage in lymphocytes at levels well below 50 micrograms/gram, the level at which initial clinical signs and symptoms of mercury poisoning occur. The first apparent biological effect with increasing MeHg hair level was the impairment of lymphocyte proliferation measured as mitotic index (MI). The relation between mercury concentration in hair and MI suggests that this parameter, an indicator of changes in lymphocytes and their ability to respond to culture conditions, may be an early marker of cytotoxicity and genotoxicity in humans and should be taken into account in the preliminary evaluation of the risks to populations exposed in vivo. This is the first report showing clear cytotoxic effects of long-term exposure to MeHg. Although the results strongly suggest that, under the conditions examined here, MeHg is both a spindle poison and a clastogen, the biological significance of these observations are as yet unknown. A long-term follow-up of these subjects should be undertaken.

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“…Betti et al, 1995) although some studies also observed changes in the levels of chromosomal aberrations, SCEs and DNA adducts in function of the sampling time (e.g. Anderson et al, 1991;Perera et al, 1992;Topinka et al, 1997). This is, most probably, because DNA damage as assessed by the comet assay reflects DNA single-strand breaks and alkali labile sites (eventually also double-strand breaks) which are small, possibly repairable DNA alterations, whereas cytogenetic damage (e.g.…”

Section: Biological Endpoints Investigatedmentioning

confidence: 95%

Seasonal variations in spontaneous levels of DNA damage; implication in the risk assessment of environmental chemicals

Verschaeve¹,

Koppen²,

Gorp³

et al. 2007

J of Applied Toxicology

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The alkaline comet assay was used to investigate DNA damage levels in white blood cells of 45 normal healthy subjects. Therefore blood was sampled at four different periods, namely in February, June, August and November of the same year. Higher DNA damage levels were found in summertime, as well as higher levels of 1-hydroxypyrene in urine in this period. This suggests a higher exposure to polycyclic hydrocarbons in the summer compared with other periods of the year. The observed seasonal variation in DNA damage levels is in agreement with some, but in contradiction with other data. Seasonal variations in DNA damage levels can easily be explained by the existence of different confounders that may influence the results of a biomonitoring study. Besides sunlight and environmental pollution, also diet, allergy and physical exercise, for example, were already identified as important influencing factors. The investigation confirms that the blood sampling period is crucial in the planning and interpretation of biomonitoring studies.

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Chromosome aberrations (CA), sister-chromatid exchanges (SCE) and mitogen-induced blastogenesis in cultured peripheral lymphocytes from 48 control individuals sampled 8 times over 2 years

Cited by 45 publications

References 25 publications

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon

Seasonal variations in spontaneous levels of DNA damage; implication in the risk assessment of environmental chemicals

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