Chromosome aberrations and micronuclei in bone marrow cells and peripheral blood lymphocytes in humans exposed to ethylene oxide

Högstedt, Benkt; Gullberg, Bo; Hedner, Karin; Kolnig, A.-M.; Mitelman, Felix; Skerfving, Staffan; Widegren, Bengt

doi:10.1111/j.1601-5223.1983.tb00585.x

Cited by 80 publications

(7 citation statements)

References 21 publications

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“…Their technique was essentially the same as that used in the present study. The difference in micronuclei frequencies between smokers and nonsmokers reported by HOGSTEDT et al (1983) was exactly the same as the one I observed (As:2.2x10-3). COUNTRYMAN and HEDDLE (1976) recommend a culture time of 96 h for lymphocyte micronuclei.…”

Section: Micronucleisupporting

confidence: 87%

Effects of ionizing radiation on chromosome aberrations, sister chromatid exchanges and micronuclei in lymphocytes of smokers and nonsmokers

Stenstrand

2008

Hereditas

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Chromosome aberrations, sister chromatid exchanges and micronuclei were studied in irradiated and unirradiated peripheral blood lymphocytes of smokers and nonsmokers. No effect of smoking could be observed in the number of chromosome aberrations, either in the irradiated or nonirradiated samples. Smokers had significantly elevated SCE values in the unirradiated samples, but the difference in SCE frequencies between smokers and nonsmokers in the irradiated series was not significant. The mean frequency of micronuclei in smokers was significantly higher than in nonsmokers. After irradiation the mean difference in micronuclei frequencies between smokers and nonsmokers was greater than the difference between the unirradiated samples of the two groups.

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Section: Micronucleisupporting

confidence: 87%

Effects of ionizing radiation on chromosome aberrations, sister chromatid exchanges and micronuclei in lymphocytes of smokers and nonsmokers

Stenstrand

2008

Hereditas

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“…Contrary to the results of MIC-affected smokers, an elevated BNMN and MNL were found in the control group smokers than non-smokers (Table 5). There are only a few studies reported the association between smoking and MN (Hogstedt et al, 1983;Hogstedt, 1984;Sorsa et al, 1988;da Cruz et al, 1994;Di Giorgio et al, 1994;De Boeck et al, 2000). With regard to alcohol consumption, we observed higher MNL only in the control group alcoholics.…”

Section: Discussionmentioning

confidence: 99%

Increased Micronucleus Frequency in Peripheral Blood Lymphocytes Contributes to Cancer Risk in the Methyl Isocyanate-Affected Population of Bhopal

Senthilkumar¹,

Akhter²,

Malla³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The Bhopal gas tragedy involving methyl isocyanate (MIC) is one of the most horrific industrial accidents in recent decades. We investigated the genotoxic effects of MIC in long-term survivors and their offspring born after the 1984 occurrence. There are a few cytogenetic reports showing genetic damage in the MIC-exposed survivors, but there is no information about the associated cancer risk. The same is true about offspring. For the first time, we here assessed the micronucleus (MN) frequency using cytokinesis-blocked micronucleus (CBMN) assay to predict cancer risk in the MIC-affected population of Bhopal. A total of 92 healthy volunteers (46 MICaffected and 46 controls) from Bhopal and various regions of India were studied taking gender and age into consideration. Binucleated lymphocytes with micronuclei (BNMN), total number of micronuclei in lymphocytes (MNL), and nuclear division index (NDI) frequencies and their relationship to age, gender and several lifestyle variabilities (smoking, alcohol consumption and tobacco-chewing) were investigated. Our observations showed relatively higher BNMN and MNL (P<0.05) in the MIC-affected than in the controls. Exposed females (EF) exhibited significantly higher BNMN and MNL (P<0.01) than their unexposed counterparts. Similarly, female offspring of the exposed (FOE) also suffered higher BNMN and MNL (P<0.05) than in controls. A significant reduction in NDI (P<0.05) was found only in EF. The affected group of non-smokers and non-alcoholics featured a higher frequency of BNMN and MNL than the control group of non-smokers and non-alcoholics (P<0.01). Similarly, the affected group of tobacco chewers showed significantly higher BNMN and MNL (P<0.001) than the non-chewers. Amongst the affected, smoking and alcohol consumption were not associated with statistically significant differences in BNMN, MNL and NDI. Nevertheless, tobacco-chewing had a preponderant effect with respect to MNL. A reasonable correlation between MNL and lifestyle habits (smoking, alcohol consumption and tobacco-chewing) was observed only in the controls. Our results suggest that EF and FOE are more susceptible to cancer development, as compared to EM and MOE. The genotoxic outcome detected in FOE reflects their parental exposure to MIC. Briefly, the observed cytogenetic damage to the MIC-affected could contribute to cancer risk, especially in the EF and FOE.

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“…These studies additionally provided some insight regarding the choice of cells and their influence on the micronucleus assay. Högstedt et al [22] in their study indicated that MN assay in bone marrow cells was a better indicator than lymphocyte MN. In some of the other studies MN formation was evaluated in exfoliated buccal and nasal cells, in addition to lymphocyte cells.…”

Section: Discussionmentioning

confidence: 98%

“…Högstedt et al [22] investigated the effect of EtO on different cytogenetic parameters in an exposed Swedish population. They studied a group of 28 workers exposed to EtO and 20 control subjects.…”

Section: Epidemiological Evidence Of Mn Formation In Workers Exposed mentioning

confidence: 99%

Genotoxicity of ethylene oxide: A review of micronucleus assay results in human population

Ghosh

Godderis

2016

Mutation Research/Reviews in Mutation Research

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Ethylene oxide (EtO) has been categorized as "carcinogenic to humans (Group 1)" by the IARC. While several epidemiological studies have reported carcinogenicity and EtO-Hb formation; information from cytogenetic endpoints are rather inconclusive. In the present review, we focus on the results of eleven studies which have reported the results of micronucleus assay in EtO exposed workers. We have critically reviewed these studies based on the exposure assessment, concentration and duration, and compared the sensitivity of micronucleus assay to other reported endpoints like EtO-Hb, CA, SCE. The levels of EtO and EtO-Hb adducts in all the studies were strongly correlated to the results of SCE, but not to MN. MN were only observed in a limited number of studies with high EtO exposure (2-28ppm 8h-TWA) and not below the recommended concentration of <1ppm. To further understand the effect of exposure of EtO on MN assay outcome, we propose studies with more harmonized protocol for exposure assessment and MN analysis, determination of suitable sample size and use of multiple target tissues to understand the effect of metabolite.

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Chromosome aberrations and micronuclei in bone marrow cells and peripheral blood lymphocytes in humans exposed to ethylene oxide

Cited by 80 publications

References 21 publications

Effects of ionizing radiation on chromosome aberrations, sister chromatid exchanges and micronuclei in lymphocytes of smokers and nonsmokers

Effects of ionizing radiation on chromosome aberrations, sister chromatid exchanges and micronuclei in lymphocytes of smokers and nonsmokers

Increased Micronucleus Frequency in Peripheral Blood Lymphocytes Contributes to Cancer Risk in the Methyl Isocyanate-Affected Population of Bhopal

Genotoxicity of ethylene oxide: A review of micronucleus assay results in human population

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