Chromosomal Variation in Crocus vernus Hill (Iridaceae) Investigated by in situ Hybridization of rDNA and a Tandemly Repeated Sequence

Frello, S.

doi:10.1006/anbo.2000.1189

Cited by 36 publications

(25 citation statements)

References 22 publications

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“…It has been widely used for chromosome identification, studying genome structure and chromosome evolution, constructing physical map of genome, investigating DNA molecular composition of specific chromosome region, analyzing the spatial location and dynamic changes of chromatin in cell cycles, etc. (De Jong et al 1999;Frello and Heslop-Harrison 2000;She and Song 2008;Paesold et al 2012;Szinay et al 2012;Sun et al 2013;Li et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Zhang

Yang

et al. 2016

Genome

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Ribosomal DNAs are useful cytogenetic markers for chromosome analysis. Studies investigating site numbers and distributions of rDNAs have provided important information for elucidating genome organization and chromosomal relationships of many species by fluorescence in situ hybridization. But relevant studies are scarce for species of the genus Cucumis, especially in wild species. In the present study, FISH was conducted to investigate the organization of 45S and 5S rDNA among 20 Cucumis accessions, including cultivars and wild accessions. Our results showed that the number of 45S rDNA sites varied from one to five pairs in different accessions, and most of these sites are located at the terminal regions of chromosomes. Interestingly, up to five pairs of 45S rDNA sites were observed in C. sativus var. sativus, the species which has the lowest chromosome number, i.e., 2n = 14. Only one pair of 5S rDNA sites was detected in all accessions, except for C. heptadactylus, C. sp, and C. spp that had two pairs of 5S rDNA sites. The distributions of 5S rDNA sites showed more variation than 45S rDNA sites. The phylogenetic analysis in this study showed that 45S and 5S rDNA have contrasting evolutionary patterns. We find that 5S rDNA has a polyploidizationrelated tendency towards the terminal location from an interstitial location but maintains a conserved site number, whereas the 45S rDNA showed a trend of increasing site number but a relatively conserved location.

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Section: Introductionmentioning

confidence: 99%

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Zhang

Yang

et al. 2016

Genome

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“…(Shibata and Hizume, 2002). According to Frello and Heslop-Harrison (2000), the number of 5S and 45S rDNA loci can vary exceptionally within a single species, as shown in Crocus vernus L. (Iridaceae). Nevertheless, the number of 5S or 45S rDNA sites is generally larger in tetraploids than in related diploids (Frello and Heslop-Harrison, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…According to Frello and Heslop-Harrison (2000), the number of 5S and 45S rDNA loci can vary exceptionally within a single species, as shown in Crocus vernus L. (Iridaceae). Nevertheless, the number of 5S or 45S rDNA sites is generally larger in tetraploids than in related diploids (Frello and Heslop-Harrison, 2000). Previous studies on Rhynchospora (Cyperaceae) showed that the number of 45S rDNA site pairs varied from two to four among diploids, and from four to 15 among polyploids (Vanzela et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situhybridization of rDNA sites and conventional stainings

Guetat¹,

Rosato²,

Rossellã³

et al. 2015

Turk J Bot

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In North Africa, Allium roseum L. has been treated as a polymorphic species comprising 12 different taxa. More than 80 individuals in the Allium roseum complex were sampled from 20 populations at different localities in Tunisia and investigated karyologically. Basic chromosome number was confirmed as x = 8. Three cytotypes, diploid (2n = 2x = 16), triploid (2n = 3x = 24), and tetraploid (2n = 4x = 32), were found. Polyploidy was recorded for the first time for this species in Tunisia. Most of the populations were diploid (17 populations), whereas two populations were tetraploid and only one was triploid. The chromosome set of studied populations is nonuniform at the species level and within cytotypes. The 45S and 5S ribosomal genes (rDNA) were visualized by fluorescent in situ hybridization for the A. roseum polyploid complex. The diploid showed three loci 45S rDNA; the triploid and tetraploid showed 9 and 12 signals, respectively. However, 5S rDNA showed 2 signals for the diploid genome and 4 signals for both triploid and tetraploid. The 45S rDNA results showed intra-and interpopulational numerical variation. However, the data obtained with the 5S rDNA genes revealed a highly conserved distribution of these ribosomal families in the chromosomes of individuals from each population, as well as among the analyzed populations.

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“…Laun. ; Brighton 1976), which refers to the newly described C. neglectus Peruzzi & Carta, is an allo-tetraploid (Frello & Heslop-Harrison 2000) formed from an hybrid between C. etruscus and C. vernus subsp. vernus with 2n = 8.…”

Section: LImentioning

confidence: 99%

Karyological study of miscellaneous Crocus (Iridaceae) species

2017

Fl. Medit.

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Chromosomal Variation in Crocus vernus Hill (Iridaceae) Investigated by in situ Hybridization of rDNA and a Tandemly Repeated Sequence

Cited by 36 publications

References 22 publications

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situhybridization of rDNA sites and conventional stainings

Karyological study of miscellaneous Crocus (Iridaceae) species

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