Chromosomal sites for marek's disease virus DNA in two chicken lymphoblastoid cell lines MDCC-MSB1 and MDCC-RP1

Hirai, Kenji; Maotani, Kumiko; Ikuta, K; Yasue, Hiroshi; Ishibashi, Masayoshi; Kato, Shiro

doi:10.1016/0042-6822(86)90390-9

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…There is evidence that the virus is latent in primary lymphomas and in the majority of the cells of several cell lines (Calnek et al, 1981 ;Rziha & Bauer, 1982;Hirai et al, 1986;Delecluse et al, 1993). In this study we have sought to uncover the viral genes that are transcribed in cell lines and have prepared cDNA libraries from the expression cell line MSB1 and also from the cell line RPL1 in which virus expression is repressed.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus

Li¹,

Pastorek

Zelnik³

et al. 1994

Journal of General Virology

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Libraries of cDNA were generated from polyadenylated RNAs derived from Marek's disease virus (MDV)-transformed cell lines by directional cloning of oligo-(dT)-primed cDNAs in lambda gt22A. Analysis of the libraries for viral sequences showed that a number of eDNA clones originated from transcripts mapping in the BamHI A region of the MDV genome. Sequencing and fine mapping of these cDNAs suggested that the RNA transcripts expressed from this region were either in the sense or antisense direction with respect to the MDV homologue of the ICP4 gene of herpes simplex virus. The longest cDNA clone from antisense transcripts was 2756 bp long and partially overlapped the 5' end of the coding region of the ICP4 gene. The eDNA clone contained at least four introns, shown by comparison of its sequence with the sequence of the ICP4 gene. The presence of introns was confirmed by PCR analysis. All the introns have the consensus splice donor and acceptor signals at their 5' and 3' ends respectively. Northern blot analysis showed that the ICP4 gene homologue of MDV was abundantly transcribed only in lytically infected fibroblasts, whereas transcripts complementary to the ICP4 gene were the major transcripts in MDVtransformed cell lines and lymphomas. The transcripts complementary to ICP4 consist of two major RNA species approximately 15 kb and 1.32kb long. The results suggest that there might be an inverse relationship between the abundance of ICP4 transcripts and their complementary transcripts in MDV-infected and transformed cells.

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Section: Discussionmentioning

confidence: 99%

Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus

Li¹,

Pastorek

Zelnik³

et al. 1994

Journal of General Virology

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“…All MDV-transformed cells are latently infected. The status of MDV DNA in the transformed cell, which is central to the virus-cell relationship, has been investigated in the past with contradictory results even with the same MDVtransformed cell line (25,26,33,55,64). Ongoing investigations with EBV and its status in Burkitt's lymphomas prompted us to study cell lines derived from Marek's disease lymphomas.…”

Section: Six Cell Lines Derived Frommentioning

confidence: 99%

Status of Marek's disease virus in established lymphoma cell lines: herpesvirus integration is common

Delecluse

Hammerschmidt

1993

J Virol

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Six cell lines derived from Marek's disease lymphomas of chickens and turkeys were investigated for the status of Marek's disease virus (MDV) DNA. In the transformed T- and B-cell lines, viral DNA could be detected by conventional Southern blot hybridization, by Gardella gel electrophoresis, and by in situ hybridization of metaphase and interphase chromosomes. Integration of viral DNA into the host cell chromosome was observed in all cell lines. Two to 12 integration sites of viral DNA could be detected in metaphase chromosome spreads. The integration sites were characteristic for the individual cell lines and were preferentially located at the telomers of large- and mid-sized chromosomes or on minichromosomes. In four of six cell lines, a minor population of latently infected cells supported the lytic cycle of MDV, giving rise to linear virion DNAs. In one of these cell lines, a third species of MDV DNA could be detected with properties reminiscent of covalently closed circular DNA. The finding that MDV integrates regularly into the genomes of latently infected cells is crucial to understanding the molecular biology of herpesvirus-induced tumors in the natural host.

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“…wt. was isolated and subjected to Southern blot hybridization with 32p-labelled cloned BamHI fragments of MDV1 DNA, as described previously (Hirai et al, 1984a(Hirai et al, , 1986b. The conditions for digestions with BamHI, HpalI and MspI were as specified by the supplier (Takara Shuzo Co., Kyoto, Japan).…”

Section: Discussionmentioning

confidence: 99%

Methylation of Marek's Disease Virus DNA in Chicken T-lymphoblastoid Cell Lines

Kanamori

Ikuta

Ueda

et al. 1987

Journal of General Virology

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Chromosomal sites for marek's disease virus DNA in two chicken lymphoblastoid cell lines MDCC-MSB1 and MDCC-RP1

Cited by 8 publications

References 20 publications

Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus

Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus

Status of Marek's disease virus in established lymphoma cell lines: herpesvirus integration is common

Methylation of Marek's Disease Virus DNA in Chicken T-lymphoblastoid Cell Lines

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