Chromosomal Replication Dynamics and Interaction with the β Sliding Clamp Determine Orientation of Bacterial Transposable Elements

Gómez, Manuel J.; Díaz-Maldonado, Héctor; González-Tortuero, Enrique; Saro, Francisco J. López de

doi:10.1093/gbe/evu052

“…For example, Tn7 appears to be drawn to replication forks by interaction with the fork-associated β-clamp (119). Indeed, a recent large-scale analysis of IS insertions in bacterial chromosomes has pointed out the potential implication of sliding clamp in targeting and provided evidence for direct interaction of various transposases with the conserved replication processing factor (120). A more detailed exploration of these results would be useful.…”

Section: Target Choicementioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

Siguier

¹

,

Gourbeyre

²

,

Varani

³

et al. 2015

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The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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“…For example, Tn7 appears to be drawn to replication forks by interaction with the fork-associated β-clamp (119). Indeed, a recent large-scale analysis of IS insertions in bacterial chromosomes has pointed out the potential implication of sliding clamp in targeting and provided evidence for direct interaction of various transposases with the conserved replication processing factor (120). A more detailed exploration of these results would be useful.…”

Section: Target Choicementioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

Siguier

¹

,

Gourbeyre

²

,

Varani

³

et al. 2015

View full text Add to dashboard Cite

The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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“…Transposition of other transposons is also coupled to replication (178)(179)(180)(181)(182)(183)(184)(185). Tn7 targets the lagging strand at a replication fork (178,179,185) using specific interactions between the Tn7-encoded auxiliary protein TnsE and the β-clamp processivity factor of the replisome (186) (see also the chapter by J. E. Peters in this volume).…”

Section: Role Of Target Replicationmentioning

confidence: 99%

The Tn 3 -family of Replicative Transposons

Nicolas

¹

,

Lambin²,

Dandoy

³

et al. 2015

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Transposons of the Tn3 family form a widespread and remarkably homogeneous group of bacterial transposable elements in terms of transposition functions and an extremely versatile system for mediating gene reassortment and genomic plasticity owing to their modular organization. They have made major contributions to antimicrobial drug resistance dissemination or to endowing environmental bacteria with novel catabolic capacities. Here, we discuss the dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives. We also provide an overview of current knowledge of the replicative transposition mechanism of the family, emphasizing most recent work aimed at understanding this mechanism at the biochemical level. Previous and recent data are put in perspective with those obtained for other transposable elements to build up a tentative model linking the activities of the Tn3-family transposase protein with the cellular process of DNA replication, suggesting new lines for further investigation. Finally, we summarize our current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products, comparing paradigm systems using a serine recombinase with more recently characterized systems that use a tyrosine recombinase.

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Chromosomal Replication Dynamics and Interaction with the β Sliding Clamp Determine Orientation of Bacterial Transposable Elements

Cited by 17 publications

References 61 publications

Everyman's Guide to Bacterial Insertion Sequences

Everyman's Guide to Bacterial Insertion Sequences

Everyman's Guide to Bacterial Insertion Sequences

The Tn 3 -family of Replicative Transposons

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