Chromosomal numerical aberrations in gastric carcinoma: Analysis of eighteen cases using in situ hybridization

Han, Kyungja; Oh, Eun‐Jee; Kim, Young S.; Kim, Yong Goo; Lee, Kyo Young; Kang, Chang Suck; Kim, Byung Kee; Kim, Won Il; Shim, Sang In; Kim, Sun Moo

doi:10.1016/s0165-4608(96)00165-3

Cited by 17 publications

(12 citation statements)

References 17 publications

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“…Han et al [23], by FISH analysis of 18 paraffin-embedded gastric adenocarcinomas, detected polysomy 8 in 27.8% and monosomy in 5.5%. In another study with centromeric probes, on 40 gastric cancer deparaffinised sections, polysomy 8 was found in 62.5% of cases [24].…”

Section: Discussionmentioning

confidence: 97%

“…Perhaps even more inexplicable according to currently prevailing gene-level models of tumorigenesis are several examples of neoplastic types characterised by multiple numerical chromosome abnormalities without concomitant structural karyotypic changes [21]. FISH studies have shown numerical aberrations, 1, 7, 8, 9, 17, 20, X and Y, to be common in primary gastric cancer [18,[22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

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Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACP01 cell line gastric adenocarcinoma

et al. 2006

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Gastric cancer is the third most frequent type of neoplasia and the second most important cause of death in the world. ACP01 is the first gastric adenocarcinoma cell line developed in Brazil. To evaluate chromosomal aberrations implicated in gastric carcinogenesis, we analysed three different passages (6th, 12th and 35th) of ACP01 cell line by fluorescence in situ hybridisation using chromosome 8 alpha-satellite probe. Most of the chromosome 8 alterations found involved a numerical increase of this chromosome. Chromosome 8 trisomy was detected in all cases, varying from 37% (6th passage) to 67% (35th passage), and chromosome 8 tetrasomy (also observed in all passages) varied from 2.5% (6th passage) to 30% (35th passage). The presence of five signals for chromosome 8 was observed in all passages with the highest frequency found in the 12th passage (20%). Our results confirm that trisomy of chromosome 8 is a common biological phenomenon in adenocarcinoma of stomach and can be used as a gastric mucosa malignancy marker. Although gastric tumours are frequent neoplasias, papers on their cytogenetics are scarce in the literature. It is, therefore, necessary to conduct new studies aiming to identify peculiar genetic characteristics of a tumour, which might help in diagnosis and prognosis of this disease, besides allowing more accurate therapeutic conduct to be established.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACP01 cell line gastric adenocarcinoma

et al. 2006

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“…One hundred lymphocytes on the same section served as controls for hybridization quality. 12–14 Split or adjacent signals were counted as one. Except for Y chromosome, only nuclei with at least one signal were analysed to avoid misinterpretation due to an insufficient hybridization.…”

Section: Methodsmentioning

confidence: 99%

“…Except for Y chromosome, only nuclei with at least one signal were analysed to avoid misinterpretation due to an insufficient hybridization. 12 …”

Section: Methodsmentioning

confidence: 99%

Chromosomal numerical aberrations are frequent in oesophageal and gastric adenocarcinomas: a study using in‐situ hybridization

Beuzen

Dubois

2000

Histopathology

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“…The 11p13-15 region has been described as being the target of frequent chromosomal rearrangements in a cytogenetic study of nine gastric and esophageal tumors (Rodriguez et al, 1990). Chromosome 11 trisomies have also been reported (Han et al, 1996), and numerical aberrations of this chromosome have been associated with worse prognosis (Kitayama et al, 2003). Within the 11p12-13 region, 13 known genes are located, among which are TRIM44 that codes for a protein containing three zinc-binding domains, and TRAF6, encoding a protein that associates with, and mediates signal transduction from, members of the TNF receptor superfamily.…”

Section: Discussionmentioning

confidence: 99%

A novel region of amplification at 11p12‐13 in gastric cancer, revealed by representational difference analysis, is associated with overexpression of CD44v6, especially in early‐onset gastric carcinomas

Carvalho

Milne

Polak

et al. 2006

Genes Chromosomes & Cancer

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Diffuse-type gastric carcinomas (GCs) are often difficult to characterize because of contamination of tumor samples by surrounding normal tissue. As such, information regarding chromosomal aberrations in this subtype of GCs is limited. In this study, we used representational difference analysis to pinpoint genomic amplifications occurring in diffuse-type GCs. We found nine differential products from two novel regions of amplification in two tumors: one product mapped to 19p13.1 and eight mapped to a 1.8-Mb region in chromosomal segment 11p12-13. These amplifications were confirmed using Southern blot analysis and occurred in 3/16 and 6/15 diffuse-type GCs, respectively. CD44, a well characterized cellular adhesion molecule involved in several human malignancies, is encoded by a gene located within 200 kb of the 11p12-13 amplification fragments. We confirmed that overexpression of isoform CD44v6 was correlated with amplification at 11p12-13 in 11/12 diffuse-type GCs. Since diffuse-type GCs occur more frequently in early-onset gastric carcinomas (EOGCs, presented at 45 years of age or younger) than in "conventional" GCs, and the tumors carrying the original amplifications were EOGCs, we investigated overexpression of CD44v6 in 107 EOGCs and 88 conventional GCs using tissue microarrays. We found frequent CD44v6 overexpression in both tumor groups (76% and 57% respectively) and, interestingly, significantly more cases with overexpression of CD44v6 in EOGCs than in conventional GCs (P = 0.005), irrespective of histology. These findings provide further evidence for both the relevance of CD44 in GC and for distinct molecular characteristics of EOGCs when compared with those of GCs occurring at a later age.

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Chromosomal numerical aberrations in gastric carcinoma: Analysis of eighteen cases using in situ hybridization

Cited by 17 publications

References 17 publications

Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACP01 cell line gastric adenocarcinoma

Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACP01 cell line gastric adenocarcinoma

Chromosomal numerical aberrations are frequent in oesophageal and gastric adenocarcinomas: a study using in‐situ hybridization

A novel region of amplification at 11p12‐13 in gastric cancer, revealed by representational difference analysis, is associated with overexpression of CD44v6, especially in early‐onset gastric carcinomas

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