Chromosomal nucleolar organizer regions in four sturgeon species as markers of karyotype evolution in Acipenseriformes (Pisces)

Fontana, Francesco

doi:10.1139/g94-126

Cited by 89 publications

(64 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we revealed that the majority of chromosomes previously detected as microchromosomes correspond to meta/submetacentrics or acrocentrics under improved karyotypic resolution. This result coincided well with the chromosome work on sturgeons using cell culture techniques, a method of precise counting and morphological characterization (Fontana, 1994;Fontana et al, 1997Fontana et al, , 1998Tagliavini et al, 1999;Fujiwara et al, 2001). The variation in chromosome number was detected among individuals in previous studies on the Acipenseriformes (Birstein et al, 1997;Fontana et al, 1997;Van Eenennaam et al, 1999;Fontana et al, 2001).…”

Section: Discussionsupporting

confidence: 85%

“…However, the present number of Ag-NORs (ϭ9-10) is not the highest score because Acipenser gueldenstaedtii (250 Ϯ 8 chromosomes) has been reported to have 13 Ag-NORs (Fontana et al, 1996). Previous studies on Ag-NORs of sturgeons have shown that sturgeon with 120 chromosomes generally had 4 (Acipenser ruthenus) or 6 (Acipenser sturio) Ag-NORs whereas species with 240 chromosomes generally represent 8 Ag-NORs (Acipenser baeri and Acipenser naccarii) (Fontana, 1994;Fontana et al, 2001). Unfortunately, the current number of Ag-NORs from shortnose species (ten species) alone cannot be an authentic proof for clear verification of either hexaploidy or dodecaploidy.…”

Section: Discussionmentioning

confidence: 87%

“…The chromosome counts of Acipenser brevirostrum showed intraindividual F rom estimations of genome size and chromosome number, polyploidization is believed to have played a major role in the evolution of sturgeons (Birstein et al, 1997;Fontana et al, 1997;Ludwig et al, 2001). The Acipenseriformes can generally be divided into the following two groups: (1) approximately 120 chromosomes representing average cellular DNA content of 3.2-4.9 pg, and (2) 240-260 chromosomes displaying 8.0-10.6 pg (Blacklidge and Bidwell, 1993;Fontana, 1994;Birstein et al, 1997;Fontana et al, 2001;Ludwig et al, 2001). In addition, two species are known to have extraordinarily large genome sizes (more than 13 pg of DNA/cell, after flow cytometry analysis); one is the shortnose sturgeon Acipenser brevirostrum (see Blacklidge and Bidwell, 1993) and the other is the Asian form of Acipenser medirostris (ϭA.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Karyotype of North American shortnose sturgeon Acipenser brevirostrum with the highest chromosome number in the Acipenseriformes

et al. 2005

View full text Add to dashboard Cite

The shortnose sturgeon Acipenser brevirostrum was revealed to have a larger number of chromosomes than previously reported for other sturgeon species. Its chromosome number ranged from 362 to 372 (of ten specimens examined), showing intraindividual variation. The karyotype of metaphase with the highest chromosome number (372) consisted of 89 pairs of macrochromosomes and 97 pairs of microchromosomes (fundamental number; NF ϭ 550). Although the microchromosomes were relatively shorter than the macrochromosomes, most of them had discernible arms and centromeres. Silver-stained nucleolar organizer regions (Ag-NORs) were localized on the telomeric regions of 5 pairs of chromosomes (Ag-NORs ϭ 10): 4 were made up of small meta/submetacentrics and 1 of acrocentrics. Polyploidy of A. brevirostrum should be hexaploid based on the karyotype, numerous chromosomes, Ag-NORs, and previously reported large genome size (ca. 13 pg DNA/cell).Supplementary material A color version of silver-stained metaphase of Acipenser brevirostrum (Fig. S1) is available on Springer's server at springerlink.com Materials and MethodsSpecimens were captive-bred and raised in the hatchery at University of Florida, Gainesville, USA. They (half-siblings) were given an intraperitoneal injection of 0.5% colchicin at a dose level of 10 µg/g body weight and then kept for 3-4 h in well-aerated water at 25°C. The kidneys were surgically removed, scissored, and subjected to hypotonic treatment (0.075 M KCl) for 20 min at room temperature. The cell suspension was fixed with cold methanol-glacial acetic acid (3 : 1). Chromosome slides were prepared using an airdrying method (Kim et al., 1995). The chromosomes were stained with Giemsa (Fluka) for karyotypic analysis. Countable metaphases were examined from ten speciemens.For the selective staining of Ag-NORs, Giemsa-stained metaphases were destained with alcohol-acetic acid mixture for 20 min followed by absolute ethanol for 1 h. NOR staining with AgNO 3 was carried out following the colloidal silver method of Howell and Black (1980). The best 6-10 metaphases exhibiting high NOR values per fish were examined for determining the number of active Ag-NORs. ResultsChromosome number and karyotype. The chromosome counts of Acipenser brevirostrum showed intraindividual IchthyologicalResearch

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Karyotype of North American shortnose sturgeon Acipenser brevirostrum with the highest chromosome number in the Acipenseriformes

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The Russian sturgeon is a tetraploid species that has ϳ250 chromosomes (22). Some important sex-related genes in lower species, such as the Dmw gene located on the W chromosome from Xenopus laevis (70,71), the Dmy gene located on the Y chromosome from Oryzias latipes (42,45), Amh located on the Y chromosome from Odontesthes hatcheri (25), and Sdy isolated from the Y chromosome from Oncorhynchus mykiss (66), showed a link between sex determination genes and sex chromosomes in lower vertebrates.…”

Section: Discussionmentioning

confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

View full text Add to dashboard Cite

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads. Physiol Genomics 48: 464 -476, 2016. First published May 6, 2016 doi:10.1152/physiolgenomics.00113.2015.-As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated Ͼ60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.

show abstract

“…Results have been compared to those obtained from A. sturio specimens of con®rmed identi®cation (two specimens from the Garonne river, France), and from several A. naccarii specimens of con®rmed identi®cation from a Spanish ®sh farm originating from ®sh stocks from Azienda AgrõÂ cola VIP (Brescia, Llanura Padana, Italy) and widely recognised in the literature as belonging to this latter species (e.g. Fontana 1994;Arlati et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Morphometric and genetic analysis as proof of the existence of two sturgeon species in the Guadalquivir river

et al. 1997

View full text Add to dashboard Cite

Morphometric and genetic methods were used to identify two sturgeon species, Acipenser naccarii Bonaparte, 1836, and A. sturio Linnaeus, 1758, captured in some of the principal rivers of the Iberian Peninsula, including the Guadalquivir. After measuring 25 Iberian specimens from a ®shery and several Spanish and Portuguese museums and applying stepwise discriminant analysis (SDA), four specimens preserved in di erent museums [two specimens from the Guadalquivir river (EBD-8173 and EBD-8174), one specimen from the Tagus river (MUC1) and one specimen from the Mondego river (MUC46B)], as well as ®ve specimens captured in the Guadalquivir river in the 1940s but not preserved (CM1, CM2, CM3, CM4 and CM5), were identi®ed as A. naccarii. After cloning and characterisation of a satellite-DNA family, HindIII, from A. naccarii genome, its absence from the genome of A. sturio was determined. Using this satellite-DNA as a genetic marker and by means of dot-blotting, we demonstrate that the DNA of the two specimens captured during the mid-1970s in the Guadalquivir river crosshybridised with HindIII satellite-DNA sequences of A. naccarii. We conclude that A. naccarii is autochthonous to the Iberian Peninsula and is not, as was previously believed, endemic to the Adriatic Sea.

show abstract

Chromosomal nucleolar organizer regions in four sturgeon species as markers of karyotype evolution in Acipenseriformes (Pisces)

Cited by 89 publications

References 12 publications

Karyotype of North American shortnose sturgeon Acipenser brevirostrum with the highest chromosome number in the Acipenseriformes

Karyotype of North American shortnose sturgeon Acipenser brevirostrum with the highest chromosome number in the Acipenseriformes

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Morphometric and genetic analysis as proof of the existence of two sturgeon species in the Guadalquivir river

Contact Info

Product

Resources

About