Chromosomal mapping of enzyme loci in the domestic cat: <i>GSR</i> to C2, <i>ADA</i> and <i>ITPA</i> to A3, and <i>LDHA-ACP2</i> to D1

Abstract

A panel of 42 rodent × cat somatic cell hybrids segregating individual cat chromosomes in different combinations was used to assign five isozyme structural loci to cat chromosomes. The feline homolog for glutathione reductase (GSR) was mapped to chromosome C2. Adenosine deaminase (ADA) and inosine triphosphatase (ITPA) were located on chromosome A3. Lactate dehydrogenase-A (LDHA) and acid phos-phatase-2 (ACP2) were reassigned to chromosome Dl. Localization of these genes increases the known feline genetic map and extends the known syntenic homologies between the cat and other mammalian species.

Comparative Gene Mapping in the Domestic Cat (Felis catus)

“…We have added 35 genes to the feline genetic map by Southern blot analyses of a feline x rodent somatic cell hybrid panel. These assignments increase the marker density of the syntenic map of the cat to 105 loci; 36 isozymes (Berman et al 1986;Gilbert et al 1988;, 35 oncogenes (Okuda et al 1993;Tsujimoto et al 1993), 12 genes involved with immune response (Cho KW, Youn HY, Cevario S, O'Brien SJ, Watari T, Tsujimoto H, and Hasegawa A, submitted; Okuda M, Minehata K, Setoguchl A, Nishigaki K, Watari T, Cevario S, O'Brien SJ, Tsujimoto H, and Hasegawa A, submitted; Yuhki and O'Brien 1988), 2 homeobox genes (Masuda et al 1991), 1 gene encoding an rRNA (Yu et al 1980), 2 genes encoding coat-color phenotypes (O'Brien et al 1986), 4 copies of the endogenous retrovlrus RD114 (Reeves et al 1985), and 13 miscellaneous genes. This increase in markers on the feline map aids disease gene analyses, the study of functional genome organization, and the extent of possible genome comparisons.…”

“…The identity of each probe was verified by confirming that the expected fragment size from human DNA for each probe was obtained by a Southern blot analysis. The development of the hybrid cell lines has been described (Berman et al 1986;Gilbert et al 1988;Masuda et al 1991;O'Brien 1986;. Hybrid lines were characterized by chromosomal G-banding and isozyme typing, which has been described (Berman et al 1986;Gilbert et al 1988;Masuda et al 1991;Nash and O'Brien 1982;.…”

Molecular analysis of HPRT1+ somatic cell hybrids derived from a carrier of anHPRT1 mutation responsible for Lesch-Nyhan syndrome

“…Three different hybrid cell panels were prepared with 1) RAG murine cells [Klebe et al, 1970] and lymphocytes of the proband, 2) RAG cells and lymphocytes of control I, and 3) AKO1/15 cells derived from Akodon cursor [Bonvicino et al, 1998, 2001] and lymphocytes of control II. Following a seven‐day growth in Dulbecco minimal essential medium (DMEM)/6MP selective medium (DMEM supplemented with 10% fetal calf serum (FCS) and 16.7 μg of 6‐mercaptopurine per ml) 3 × 10 6 recipient cells were fused with 1.2 × 10 7 human lymphocytes following the procedure described by Berman et al [1986]. Hybrid cells were selected in DMEM/HAT medium (DMEM with 10% FCS, hypoxanthine (100 μM), aminopterin (0.4 μM), and thymidine (16 μM)).…”

Sheep gene mapping by somatic cell hybridization

“…In this paper, we report results obtained with 3 other enzyme markers: aconitase 1 (AC01), inosine triphosphatase (ITPA) and glyceraldehyde-3-phosphate dehydrogenase (GAPD); and 6 DNA markers: ornithine transcarbamylase (OTC), color vision red pigment (RCB), raf oncogene (ARAF1) and /3-gene locus DR of human lymphocyte antigen region (HLA-DR!). ENZYME MARKERS AC01, ITPA and GAPD were studied by cellulose acetate electrophoresis using modified versions of the methods described by Womack and Moll (1986 (Hopkinson et al, 1976), hamster (Stalling et al, 1982), mouse (Siciliano et al, 1984), cat (Berman et al, 1986), cattle (Womack and Moll, 1986) (Bootsma and Ruddle, 1978;Ruddle and Meera Khan, 1976), cattle (Womack and Moll, 1986) …”