Chromosomal locations of twelve isozyme loci in Pisum sativum

Weeden, N. F.; Marx, Gerard

doi:10.1093/oxfordjournals.jhered.a109958

Cited by 37 publications

(15 citation statements)

References 7 publications

(7 reference statements)

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“…The pair of tester lines used in this study were developed with different alleles at over twenty isozyme loci (21,22). In addition, each tester line possesses distinctive morphological mutations.…”

Section: Resultsmentioning

confidence: 99%

sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum

Kneen¹,

LaRue²,

Hirsch³

et al. 1990

Plant Physiol.

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Treatment of Pisum sativum (L.) cv. 'Sparkle' with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Compared with the nitrogen fixing nodules of the parent, the zones are smaller and the nodules senesce prematurely. Bacteroids in E135F are less elongated and less differentiated than those in 'Sparkle.' The E135F mutant forms ineffective nodules when inoculated with nine different effective strains of Rhizobium leguminosarum and also when grown in a soil containing effective strains. The ineffective phenotype of E135F is under monogenic recessive control; the gene is designated sym 13. sym 13 was located on chromosome 2 by linkage with genes for shikimic dehydrogenase and esterase-2. The original selection E135F carried another mutation in heterozygous form at a separate locus, yielding some homozygous recessive nonnodulating progeny, E135N, in (14) with ineffective nodules have been discovered in breeding programs. In addition, ineffective lines of chickpea (Cicer arietinum L.) (1), faba bean (Vicia faba L.) (4), and pea (Pisum sativum L.) (3, 15) have been obtained by induced mutagenesis.This report describes an ineffective mutant of pea obtained

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Section: Resultsmentioning

confidence: 99%

sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum

Kneen¹,

LaRue²,

Hirsch³

et al. 1990

Plant Physiol.

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“…These include the primitive cv. Afghanistan (Kneen and LaRue 1984b) and the testerlinesNGB1446 (courtesy of Dr. S.Blixt, Nordic Genebank), A1078-234 (Weeden and Marx 1984), unpublished), and Fast, Slow, and NFST (Weeden NF, unpublished).…”

Section: Methodsmentioning

confidence: 99%

Non-nodulating Mutants of Pisum Sativum (L.) cv. Sparkle

Kneen

Weeden

LaRue

1994

Journal of Heredity

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Eleven pea mutants, displaying a greatly reduced number of root nodules or lacking such nodules completely, were obtained by screening the M 2 progeny of mutagenized Pisum sativum cv. Sparkle. The mutant alleles conditioning the altered nodulation phenotypes were recessive to the wild-type alleles. Eight of the mutants possessed a normal growth habit except for the complete lack of nodules. Pairwise crosses among these mutants indicated that five distinct loci had been affected. The remaining three mutants formed few nodules and also had altered root or shoot growth habit. Each of these pleiotropic mutants was coded by a distinct gene. The eight genes identified are designated sym7, sym8, sym9, sym10, sym11, sym15, sym16, and sym17, signifying their involvement in the pea/Rhizobium symbiosis. The locations of most of these sym genes were determined by classical linkage mapping. The loci were distributed on at least five of the seven chromosomes.

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“…Isozyme phenotypes were determined by horizontal starch gel electrophoresis as described in Weeden and Marx (22).…”

Section: Methodsmentioning

confidence: 99%

Inheritance, organization, and mapping of rbcS and cab multigene families in pea

Polans

Weeden

Thompson

1985

Proc. Natl. Acad. Sci. U.S.A.

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DNA restriction endonuclease fragment patterns corresponding to both the rbcS and cab multigene families of pea are each shown to segregate as single Mendelian units in the F2 progeny of two separate crosses. All of the observed variation in each of the multigene families is thus organized on the chromosome in a tightly linked complex. Linkage relationships between both multigene families and an array of morphological and isozyme markers establish the location of the rbcS and cab gene clusters on pea chromosomes 5 and 2, respectively. Our results, which indicate a high level of DNA restriction fragment length polymorphism in pea, suggest sufficient variation to permit the construction of a highly detailed linkage map.Two of the most prominent and abundant chloroplast proteins, the small subunit of ribulose-1,5-bisphosphate carboxylase and the light-harvesting chlorophyll a/b binding protein, are specified by nuclear genes (rbcS and cab, respectively) (1, 2) whose transcript levels are light-regulated (3-6). The isolation of cDNA and genomic clones corresponding to rbcS and cab has facilitated investigations into their structure and expression in pea (5, 7-12), soybean (13), petunia (14,15), and wheat (16). In particular, evidence has been accumulated that both proteins are encoded by small multigene families (7,(9)(10)(11)(13)(14)(15)(16)(17). In this study the inheritance of restriction endonuclease fragment length variants containing genes belonging to either the rbcS or cab multigene family of pea is described, and genes encoding both families are mapped onto the pea genome. To the best of our knowledge, the use of DNA restriction fragment length polymorphism to link plant structural genes to markers on a genetic map has not been reported previously. MATERIALS AND METHODSPlant Material. Seed for Pisum sativum L. inbred lines was obtained through the courtesy of G. A. Marx (New York State Agricultural Experiment Station). These accessions are labeled 1-11 as follows: (1) A778-26-6, (2) A73-91, (3) A1179-395, (4) B980-686, (5) C879-344, (6) A1078-236, (7) A78-237, (8) C482-236, (9) A578-238, (10) A780-388, (11) A1078-234. F2 progeny were obtained by crossing C879-344 with A73-91 and A73-91 with A78-237. The 43 F2 individuals resulting from the first cross and the 31 F2 individuals resulting from the second cross were each surveyed for DNA restriction fragment length variants, isozymes, and morphological characters.DNA Methodology. DNAs were extracted from the leaves of individual pea plants in a rapid, small-scale procedure (18) (20) following procedures outlined in New England Nuclear catalog NEF-972, specific rbcS and cab sequences were localized by hybridization with nick-translated pea cDNA clones pSS15 and pAB96 (kindly provided by N.-H. Chua), which correspond to the small subunit of ribulose-1,5-bisphosphate carboxylase and polypeptide 15 of the chlorophyll a/b light-harvesting complex, respectively (12). Nicktranslation and hybridization generally followed the protocols of Maniatis et al. (21). ...

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Chromosomal locations of twelve isozyme loci in Pisum sativum

Cited by 37 publications

References 7 publications

sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum

sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum

Non-nodulating Mutants of Pisum Sativum (L.) cv. Sparkle

Inheritance, organization, and mapping of rbcS and cab multigene families in pea

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