Chromosomal Location of a Gene Defining Nicotinamide Deamidase in
            <i>Escherichia coli</i>

Dickinson, Ellen S.; Sundaram, T. K.

doi:10.1128/jb.101.3.1090-1091.1970

Cited by 22 publications

(8 citation statements)

References 8 publications

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“…Penicillin selection procedures were used to isolate nadC mutants capable of growing on NA but incapable of growing on NAm. The mutation is located at 39 min on the E. coli linkage map (7,24) and is designated pncA. Extracts from pncA mutants, as predicted, lacked NAm deamidase.…”

Section: Pyridine Nucleotide Cyclementioning

confidence: 82%

Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.

Foster¹,

Moat²

1980

Microbiological Reviews

145

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Section: Pyridine Nucleotide Cyclementioning

confidence: 82%

Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.

Foster¹,

Moat²

1980

Microbiological Reviews

145

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“…This has been termed the pyridine nucleotide cycle by Gholson (3). Only one gene involved in this cycle, pncA, has been mapped (2). The enzyme coded for by the pncA gene is involved with the conversion of nicotinamide to nicotinic acid.…”

mentioning

confidence: 99%

Cross-Feeding of Escherichia coli Mutants Defective in the Biosynthesis of Nicotinamide Adenine Dinucleotide

Kerr

Tritz

1973

J Bacteriol

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Mutants of Escherichia coli defective in the biosynthesis of nicotinamide adenine dinucleotide (NAD) are able to grow in a Casamino Acids medium lacking NAD and its immediate precursors, nicotinic acid and nicotinamide. This property has allowed the development of a system to measure cross-feeding between a nadA and a nadB mutant. This system provides a means of isolating the intermediate, prequinolinic acid, as well as a biological assay for the compound. The nadB mutant feeds the nadA mutant, indicating that the nadA enzyme occurs first in the pathway and the nadB enzyme second. No cross-feeding was detected between nadA and nadC or between nadB and nadC.

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“…(iii) The construct with multiple plasmid copies of pncA was able to grow with NAM as a sole nitrogen source. (iv) The E. coli NAMase gene was previously mapped to 39 min, the same location as pncA (4,5,10,19). (v) The deduced PncA amino acid sequence has a region similar to the active site of a known amidohydrolase.…”

Section: Discussionmentioning

confidence: 99%

“…1) as a part of NAD recycling. The Escherichia coli NAMase gene (pncA) (synonym, nam) has been mapped to 39 min (4,5,10). Circumstantial evidence suggests that a single M. tuberculosis enzyme has both PZAase and NAMase activities (21).…”

mentioning

confidence: 99%

Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA

Frothingham¹,

Meeker-O'Connell

Talbot

et al. 1996

Antimicrob Agents Chemother

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Pyrazinamide (PZA) is one of the three most important drugs for treatment of Mycobacterium tuberculosis infections. The antibacterial activity of PZA requires a bacterial enzyme, pyrazinamidase (PZAase), which hydrolyzes PZA to form pyrazinoic acid and ammonia. Most PZA-resistant clinical M. tuberculosis isolates lack PZAase activity. With the goal of eventually identifying and characterizing the M.tuberculosis PZAase gene, we began with the more tractable organism, Escherichia coli, which also has PZAase activity. We screened a transposon-generated E. coli insertion mutant library, using a qualitative PZAase assay. Two PZAase-negative mutants out of 4,000 colonies screened were identified. In each mutant, the transposon interrupted the same 639-bp open reading frame (ORF), ORF1. The expression of ORF1 on a multicopy plasmid complemented a PZAase-negative mutant, leading to PZAase activity levels approximately 10-fold greater than those of the wild type. PZA has a structure similar to that of nicotinamide, a pyridine nucleotide cycle intermediate, so we tested our strains for nicotinamidase activity (EC 3.5.1.19) (genetic locus pncA). The construct with multiple plasmid copies of ORF1 had an approximately 10-fold increase in levels of nicotinamidase activity. This overexpressing strain could utilize nicotinamide as a sole nitrogen source, through wild-type E. coli cannot. We conclude that a single E. coli enzyme accounts for both PZAase and nicotinamidase activities and that ORF1 is the E.coli PZAase and nicotinamidase gene, pncA.

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Chromosomal Location of a Gene Defining Nicotinamide Deamidase in Escherichia coli

Abstract: A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12.

Cited by 22 publications

References 8 publications

Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.

Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.

Cross-Feeding of Escherichia coli Mutants Defective in the Biosynthesis of Nicotinamide Adenine Dinucleotide

Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA

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