Chromosomal localization of the human proto-oncogene c-ets

Taisne, C. de; Gégonne, Anne; Stéhelin, D; Bernheim, Alain; Berger, Roland

doi:10.1038/310581a0

Cited by 175 publications

(52 citation statements)

References 22 publications

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“…60 kd present in both AMV-and E26-transformed cells was also recognized by anti-bp9O but not by anti-bp39 (lanes 3 and 7). Paradoxically, anti-bp9O activity against this polypeptide could be removed by absorption with purified bp39 (lanes 4 and 8) 8) myeloblasts. The immunoprecipitates were separated on a 7.5% polyacrylamide gel which was dried and then exposed to X-ray film.…”

Section: Resultsmentioning

confidence: 99%

The proto-oncogene c-ets is preferentially expressed in lymphoid cells.

Chen¹

1985

Mol. Cell. Biol.

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The transforming sequences of the avian acute leukemia virus, E26, contain two distinct oncogenes, v-mybE and v-ets, fused together. By using a probe containing v-ets sequences, polyadenylated transcripts of the c-ets proto-oncogene were detected in avian tissues; they included a major 7.0-kilobase and a minor 2.0-kilobase species. These c-ets mRNAs were detected at high levels only in lymphoid organs and in avian T and B lymphoid cell lines. A similar pattern of c-ets transcription was observed in human hematopoietic cell lines, with transcripts detected in lymphoid B and T cells but not in erythroid or myeloid cells. The E26 oncogene was inserted into an inducible expression vector, and a 90-kilodalton protein (bp9O) was produced in bacteria. Rabbit antisera raised to purified bp9O precipitated p135gamYybEets, the v-mybE-ets polyprotein expressed in E26-transformed cells, and also reacted with p50vmsbA, the transforming protein of the avian myeloblastosis virus. Antiserum to bp9O was absorbed with a bacterially synthesized v-mybA protein to remove anti-myb activity. The absorbed anti-bp9O serum retained the ability to immunoprecipitate p135gag9-YbetS from E26-transformed cells and specifically reacted with a 56-kilodalton polypeptide (p56) detected in chicken lymphoid organs and in T and B lymphocytes of both avian and human origin. The data suggest that p56 is a translational product of the c-ets proto-oncogene and imply that p56 may be involved in regulating the growth of lymphoid cells.

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Section: Resultsmentioning

confidence: 99%

The proto-oncogene c-ets is preferentially expressed in lymphoid cells.

Chen¹

1985

Mol. Cell. Biol.

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“…c-Ets was initially identified as the proto-oncogene corresponding to the v-ets oncogene of the E26 avian retrovirus (Leprince et al 1983;Nunn et al 1983;de Taisne et al 1984). Ets-1 is the archetypal member of the Ets family.…”

Section: Activation Of Dna-binding Activitymentioning

confidence: 99%

Molecular basis of tissue‐specific gene expression mediated by the Runt domain transcription factor PEBP2/CBF

1999

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The Runt domain transcription factor PEBP2/CBF is a heterodimer of ␣ and ␤ subunits. Of the three mammalian ␣ subunit genes, which are homologues of the Drosophila gene runt, PEBP2␣A/CBFA1 and PEBP2␣B/AML1 are critical regulators of osteogenesis and haematopoiesis, respectively. A unique functional interaction between PEBP2␣B/AML1 and Ets-1 was recently observed, and provides a clear example of context-dependent transcriptional regulation. An elaborate mechanism of cooperation between the two factors may represent a basis for tissuespecific gene expression, which is thought to be achieved by combinations of specific sets of transcription factors unique to each cell lineage or stage of differentiation. A possible molecular basis for the critical role(s) played by PEBP2/CBF in tissue determination is discussed in light of these observations.

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“…The cellular counterpart of v-ets, c-ets, is clearly distinct from c-myb since it is transcribed in some normal cell types as a major 7.5-kilobase (kb) polyadenylated RNA (as well as two minor 2.2-and 1.5 (2.0)-kp species) in contrast to the 4.0-kb c-myb mRNA (16,27). Furthermore, the human cellular genes c-myb and c-ets have been assigned to different chromosomes: 6q22-24 for c-myb (14), 11q23-24 for c-ets-J (8,26), and 21q22.1-22.3 for c-ets-2 (26). The E26 viral transforming protein is a 135,000-Mr fusion protein, P135gag-myb-e:s translated from the 5.7-kb genomic viral RNA and located in the nucleus of E26-transformed cells (2,4,5,15).…”

mentioning

confidence: 99%

Multiple domains for the chicken cellular sequences homologous to the v-ets oncogene of the E26 retrovirus.

Gegonne¹,

Leprince²,

Duterque-Coquillaud³

et al. 1987

Mol. Cell. Biol.

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We have investigated the structure of chicken genomic DNA homologous to v-ets, the second cell-derived oncogene of avian retrovirus E26. We isolated a c-ets locus spanning ca. 30.0 kilobase pairs (kbp) in the chicken genome with homologies to 1,202 nucleotides (nt) of v-ets (total length, 1,508 nt) distributed in six clusters along 18.0 kbp of the cloned DNA. The 5'-distal part of v-ets (224 nt) was homologous to chicken cellular sequences contained upstream within a single 16.0-kbp EcoRI fragment as two typical exons but not found transcribed into the major 7.5-kb c-ets (or 4.0-kb c-myb) RNA species. Between these two v-ets-related cellular sequences we found ca 40.0 kbp of v-ets-unrelated DNA. Finally, the most 3' region of homology to v-ets in the cloned DNA was shown to consist of a truncated exon lacking the nucleotides coding for the 16 carboxy-terminal amino acids of the viral protein but colinear to one of the two human c-ets loci, c-ets-2.

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Chromosomal localization of the human proto-oncogene c-ets

Cited by 175 publications

References 22 publications

The proto-oncogene c-ets is preferentially expressed in lymphoid cells.

The proto-oncogene c-ets is preferentially expressed in lymphoid cells.

Molecular basis of tissue‐specific gene expression mediated by the Runt domain transcription factor PEBP2/CBF

Multiple domains for the chicken cellular sequences homologous to the v-ets oncogene of the E26 retrovirus.

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