Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization

Abstract

The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5+/-1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2+/-1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.

From genome to proteome: great progress in the domesticated silkworm (<italic>Bombyx mori</italic> L.)

“…Song et al reported the chromosomal locations of two single-copy genes, Ser-1 and CI-13, in B. mori by FISH. The results showed that Ser-1 was located near the distal end of the 11th linkage group with a relative position of 12.5±1.4 in pachytene, while CI-13 was mapped near the distal end of the second linkage group with a relative position of 8.2±1.2 in pachytene [22].…”

A Comparative Study of 80r and 240r Gamma Rays On Male Meiotic Chromosomes of Grasshopper Spathosternum Praciniferum

“…This paved the path to succeeding workers towards cytogenetic study of radiation induced chromosomal aberration in insects, as Carlson (1938Carlson ( , 1940Carlson ( , 1941aCarlson ( , 1941bCarlson ( , 1949, Carlson & Harrington (1955), Ray-Choudhuri (1961), Manna and Majumdar (1962,1968, Majumdar and , Kumaraswammy & Rajasekarasetty (1975, 1976, Rajasekarasetty & Kumarwaswamy (1979), Sur and Manna (1987), Sur (1998), Sur and Pandey (2004). Very recently, Song et al (2008) mapped the chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) by fluorescence in situ hybridization. Even, Dutrillauxet al (2006) had reported a method for obtaining chromosome banding, from pachytene stage of the meiotic prophase in Coleopteran species, which will be of help in further cytogenetic studies by other workers.…”