Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Pan, Cuili; Li, Qingfeng; Lu, Yuqing; Zhang, Jinpeng; Yang, Xiaoling; Li, Xiuquan; Li, Lihui; Liu, Weihua

doi:10.1371/journal.pone.0175265

Cited by 21 publications

(20 citation statements)

References 44 publications

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“…Wheat– A. cristatum alien addition/substitution lines were obtained from several generations of backcrossing or selfing following the hybridization of the common wheat Fukuho with A. cristatum accession ‘Z559’ (2 n = 4 x = 28, PPPP) (Table ). Among these lines, II‐3‐1, II‐9‐1, II‐8‐1, II‐7‐1, II‐21‐2, II‐21‐6, 5113‐2, 5114‐3, 4844‐12, 4844‐8, 5038, 5043, II‐5‐1 and II‐26‐1 were identified previously (Han et al ., ; Pan et al ., ; Wu et al ., ). ‘Z1842’, ‘Z2098’ and their 119 progeny, Fukuho, ‘Z559’ and addition/substitution lines were planted in the glasshouse at the Chinese Academy of Agricultural Sciences, Beijing, China.…”

Section: Plant Materialsmentioning

confidence: 99%

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Zhou

Zhang

Che

et al. 2017

Plant Biotechnology Journal

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Summary Agropyron Gaertn. (P genome) is a wild relative of wheat that harbours many genetic variations that could be used to increase the genetic diversity of wheat. To agronomically transfer important genes from the P genome to a wheat chromosome by induced homoeologous pairing and recombination, it is necessary to determine the chromosomal relationships between Agropyron and wheat. Here, we report using the wheat 660K single nucleotide polymorphism (SNP) array to genotype a segregating Agropyron F1 population derived from an interspecific cross between two cross‐pollinated diploid collections ‘Z1842’ [A. cristatum (L.) Beauv.] (male parent) and ‘Z2098’ [A. mongolicum Keng] (female parent) and 35 wheat–A. cristatum addition/substitution lines. Genetic linkage maps were constructed using 913 SNP markers distributed among seven linkage groups spanning 839.7 cM. The average distance between adjacent markers was 1.8 cM. The maps identified the homoeologous relationship between the P genome and wheat and revealed that the P and wheat genomes are collinear and relatively conserved. In addition, obvious rearrangements and introgression spread were observed throughout the P genome compared with the wheat genome. Combined with genotyping data, the complete set of wheat–A. cristatum addition/substitution lines was characterized according to their homoeologous relationships. In this study, the homoeologous relationship between the P genome and wheat was identified using genetic linkage maps, and the detection mean for wheat–A. cristatum introgressions might significantly accelerate the introgression of genetic variation from Agropyron into wheat for exploitation in wheat improvement programmes.

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Section: Plant Materialsmentioning

confidence: 99%

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Zhou

Zhang

Che

et al. 2017

Plant Biotechnology Journal

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“…In particular, An et al [31] obtained a new wheat-rye addition line called WR35 via crossing Xiaoyan 6 with the rye cultivar German White, where the addition line exhibited resistance to powdery mildew and stripe rust, and a high kernel number per spike. Pan et al [32] also found that the 1P chromosome from Agropyron cristatum enhanced the spike length and tiller number in wheat, and a wheat-A. cristatum 1P addition line was obtained using molecular and phenotypic identification techniques.…”

Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

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“…This technique allows for the genome-specific chromosome labeling in the cells of allopolyploid or hybrid species. It has been widely used in plant studies to identify putative parental genomes in the interspecific or intergeneric hybrids or allopolyploids (for the review see Chester et al 2010;Silva and Souza, 2013), to study the evolution of polyploid genomes (Song et al 1995;Gaeta et al 2007;Majka et al 2018;Tan et al 2017), or to detect the introgression of alien chromatin (Schneider et al 2005;Tan et al 2005) Manuscript to be reviewed as translocation (Kruppa et al 2013), addition (Molnar-Lang et al 2000;Ji and Chetelat 2007), or substitution (Pan et al 2017) lines.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #2 of "Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.) (v0.1)"

2018

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Background: The oat × maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids. Methods: Oat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande-1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH). Results: A total of 138 oat lines obtained by crossing of 2314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in 8 lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed 2 maize chromosomes similar in size, three OMA-one maize chromosome, and one OMA-four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1-102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced

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Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Cited by 21 publications

References 44 publications

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Peer Review #2 of "Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.) (v0.1)"

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