Chromosomal localization of autosomal mutations inDrosophila nasuta nasuta andDrosophila nasuta albomicans

Abstract

By analyzing the progeny of crosses involving brown eye mutants and the wild types in two members of Drosophila nasuta subgroup namely D. n. nasuta and D. n. albomicans we could show that the mutant gene is recessive, located in the chromosome 2 and the alleles of this gene are present at different loci. A study of fitness in the eye color mutants in comparison with the wild types revealed that D. n. nasuta mutant has higher viability at both 25 ± 1 • C and ambient temperatures; while D. n. albomicans mutant has faster rate of development only at 25 ± 1 • C. Quantitative analysis of eye pigments in the mutants revealed that there is biosynthesis of both pteridines and xanthommatins unlike in bw/bw of D. melanogaster, where only xanthommatins are synthesized. In both the species, the pteridine quantities in mutants are similar; whereas xanthommatin quantity in bw n /bw n is 10 times higher than that of bw a /bw a. Further, the F 1 progeny of intraspecific crosses (wild type X mutant) are found to have high amounts of pteridine, even when compared with parental wild type.

ACUTE EXPOSURE OF Drosophila melanogaster TO PARAQUAT CAUSES OXIDATIVE STRESS AND MITOCHONDRIAL DYSFUNCTION

“…The flies were maintained at 25 ± 1°C and 70–80% relative humidity, and fed on a standard wheat flour–agar diet with yeast granules as a protein source. Diet was prepared according to a standard protocol (Ashadevi and Ramesh, ).…”

Last mated male sperm precedence in doubly mated females is not ubiquitous: evidence from sperm competition in laboratory populations of Drosophila nasuta nasuta and Drosophila nasuta albomicans

“…The brown mutation in D. n. nasuta and D. n. albomicans is not isolocus and therefore reciprocal crosses conducted between brown of D. n. nasuta and brown of D. n. albomicans yields only wild-type phenotype (Ashadevi et al 2005). Hence, the competition between conspecific and heterospecific sperm and the pattern of sperm precedence in the F 1 hybrids could not be included for the present study and thus we focussed only on intraspecific sperm competition involving these two subspecies.…”