Chromosomal Localization of 5S and 18S rDNA in Five Species of Subgenus Strobus and their Implications for Genome Evolution of Pinus

Cai, Qingsheng; Liu, Zhan‐Lin; Wang, Xiaoru

doi:10.1093/aob/mcl030

Cited by 79 publications

(63 citation statements)

References 53 publications

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“…Data on banding patterns can be used for comparative karyotyping in some species, and they detect inter-and intraspecific variation patterns (Hizume et al 1983(Hizume et al , 1989(Hizume et al , 1990; however, fluorescent banding does not provide sufficient information for the discrimination of all homologous chromosome pairs. Not all homologous pairs in Pinus species were identified by probe signals in studies of karyotypes by FISH that used 35S rDNA and 5S rDNA probes (Doudrick et al 1995, Liu et al 2003, Cai et al 2006, Bogunić et al 2011, telomere sequences (Fuchs et al 1995, Shibata et al 2005, Islam-Faridi et al 2007, or simple sequence repeats (SSR) (Pavia et al 2014). Combined fluorescent banding and FISH investigations have identified interstitial and proximal CMA bands that are consistent with 35S rDNA and/or the short repetitive sequence proximal CMA band-specific repeat (PCSR) loci, and interstitial DAPI bands that are consistent with signals of repetitive sequences containing the Arabidopsis-type telomere sequence (Hizume et al 2001, Shibata et al 2005.…”

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confidence: 99%

A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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Summary Every species has a unique karyotype, but certain genera have common karyptypes among species. The markers (chromosome length and centromere position) used in traditional karyotyping do not distinguish all chromosome pairs in the genus Pinus. However, the application of multi-probe fluorescence in situ hybridisation (FISH) procedures allowed exact karyotyping of 26 Pinus congeners. We used these new data to examine species relationships. The 35S rDNA and 5S rDNA, Arabidopsis-type telomere repeat sequences, and the proximal CMA band-specific repeat (PCSR) of P. densiflora were used as FISH probes for our analysis of chromosomes in 26 Pinus species. Each species had a unique FISH karyotype and most homologous chromosome pairs were identified. The FISH karyotypes were used to compare corresponding or homologous chromosomes among the species. Common or similar FISH signal patterns appeared in closely related species. Species that had inherited common FISH signal patterns were classified into four karyotype groups. We used cluster analyses to compare quantitative differences in FISH signals within these groups. The results of these analyses were consistent with recent systematic interpretations and resolved differences among existing taxonomic systems based on diverse methodologies. Our results indicate that FISH signal patterns reflect the history of species differentiation and that comparative FISH karyotyping has potential as an important tool for studying the taxonomy or phylogeny of Pinus.

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confidence: 99%

A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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“…Molecular cytogenetic studies have performed in seven species of subgenus Strobus (Cai et al 2006;Shibata et al 2016). All interstitial FISH signals of 45S rDNA probe seems correspond to thick CMA-bands as reported in subgenus Pinus (Hizume et al , 2001(Hizume et al , 2002b and Strobus (Cai et al 2006;Shibata et al 2016).…”

Section: Chromosome Banding In the Genus Pinusmentioning

confidence: 58%

“…Conserved karyotype is suitable for precise comparative karyotype analysis after an identification of each homoeologous chromosome and expected to supply valuable phylogenetic information. For chromosome identification of each homologous pair in chromosome complement, several techniques such as C-banding (Borzan and Papeš 1978;Tanaka and Hizume 1980;MacPherson and Filion 1981), G-banding (Drewry 1982), fluorescent banding (Hizume et al 1983(Hizume et al , 1989b(Hizume et al , 1990Jacobs et al 2000;Islam-Faridi et al 2007) and in situ hybridization (ISH) including fluorescent in situ hybridization (FISH) of rDNA probes Doudrick et al 1995;Lubaretz et al 1996;Jacobs et al 2000;Liu et al 2003;Cai et al 2006;Islam-Faridi et al 2007;Shibata et al 2016) and, other probes such as PCSR and telomere sequences (Hizume et al 2002b;Islam-Faridi et al 2007;Shibata et al 2016) were used for chromosome analysis in Pinus species. The fluorescent banding using CMA and DAPI displayed many fluorescent bands at interstitial and/or centromeric regions of most chromosomes in Pinus.…”

Section: Abstract: Chromosome Fluorescent Banding Haploxylone Pinementioning

confidence: 99%

“…Their band patterns were useful for chromosome identification and comparative karyotype analysis which revealed relationships among three Japanese species in subgenus Pinus (Hizume et al 1983(Hizume et al , 1989b(Hizume et al , 1990. Recently the FISH with two or more probes was adapted to species of subgenera Pinus (Hizume et al 2002b;Liu et al 2003;Islam-Faridi et al 2007;Shibata et al 2016) and Strobus (Cai et al 2006;Shibata et al 2016). Shibata et al (2016) showed a usefulness of multi FISH for chromosome identification and comparative karyotype analysis in many Pinus species.…”

Section: Abstract: Chromosome Fluorescent Banding Haploxylone Pinementioning

confidence: 99%

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Chromosome banding in the genus Pinus IV. Fluorescent banding patterns of chromosomes in eight taxa of haploxylone pines

et al. 2016

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Somatic chromosomes of seven taxa of Asian haploxylon pines, Pinus armandii var. amamiana, P. armandii var. armandii, P. parviflora, P. pumila, P. x hakkodensis, P. koraiensis and P. bungeana, and a American species of P. edulis were observed by a fluorescent banding method using chromomycin A 3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI). The chromosome number of all taxa was 2n=24 and their karyotypes were composed of 22 long metacentric chromosomes and two short heterobrachial chromosomes as previous reports. CMA-bands appeared at the interstitial region and not at the proximal region of metacentric chromosomes. Interstitial CMA-bands were observed on 16-18 long metacentric chromosomes in chromosome complement of most species excepting for P. bungeana having six interstitial CMA-bands. The short chromosomes of most species have an interstitial CMA-band on their long arms and that of P. edulis had a proximal CMA-band. Clear proximal CMA-or DAPI-bands appeared in most species of subgenus Pinus were not observed in species of subgenus Strobus examined. DAPI-dots appeared at centromeric regions of all chromosomes and many thin DAPI-bands did at interstitial regions as appeared in the species of subgenus Pinus. The karyotype analysis combined with CMA-and DABI-bandings was useful for identification of each homologous chromosome and for chromosomal relationship among species in haploxylon pines.

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“…Comparison of chromosomes of related plant species by using the rDNA as a marker has revealed many processes of chromosome evolution, including structural rearrangements, such as inversions and translocations (Levin 2002), as well as an extensive indication about the phylogenetic and genomic relationship (Robledo et al 2009). Modern cytogenetic tools, such as fluorescence in situ hybridization (FISH) techniques, have been widely used to resolve the chromosome evolution history and enhance knowledge of plant genome differentiation (D'Hont et al 1996(D'Hont et al , 2005Cai et al 2006). The most common application of FISH is the location of rDNA families on the chromosome as additional karyotype features to identify and track changes in chromosome structure during evolution (Mukai et al 1991;Frello and Heslop-Harrison 2000).…”

Section: Introductionmentioning

confidence: 99%

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

et al. 2012

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In this study, we analyzed 12 species in the subgenus Potamogeton (8 tetraploids and 2 diploids, 2 putative tetraploid hybrids) at the chromosomal level, including counting the chromosome number and physically mapping the rDNA. The extent of variation in the chromosome number and rDNA loci was determined in the Potamogeton species on both the inter-and intra-specific level. Moreover, one of the hybrid sets (P. perfoliatus # 9 P. wrightii $) was picked for performing artificial pollination for producing F1 generation adults and for sequential morphological character analysis and FISH detection. After comparing the parental species and natural hybrids from three different geographic locations, the intraspecific variations of rDNA loci were revealed in sampled plants; in addition, comparisons between artificial F1 and natural hybrids (P. 9 intortusifolius) showed a rapid change of 45S rDNA loci in response to the interspecific hybridization. We also compared our results concerning rDNA patterns with phylogenetic documents to derive complementary clues to karoytype evolution and interspecific relationships. Based on frequent hybridizations and active clonal reproduction with weak genetic selection, the rDNA chromosomal repatterning, such as the gain or loss of rDNA loci together with rDNA movements, might be one trend of chromosome evolution in this genus.

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Chromosomal Localization of 5S and 18S rDNA in Five Species of Subgenus Strobus and their Implications for Genome Evolution of Pinus

Cited by 79 publications

References 53 publications

A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

Chromosome banding in the genus <i>Pinus</i> IV. Fluorescent banding patterns of chromosomes in eight taxa of haploxylone pines

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

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