Chromosomal gene transfer of human cytosol thymidine kinase into mouse cells

Willecke, Klaus; Mierau, Rudolf; Kruger, Almut; Lange, Rita

doi:10.1007/bf00266614

Cited by 14 publications

(3 citation statements)

References 33 publications

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“…In stable transformants, the transgenome is associated with recipient cell chromosomes (5,7,8). The nature of the transgenome in unstable cells is uncertain and interpretation is complicated by conflicting experimental observations.…”

mentioning

confidence: 99%

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Klobutcher¹,

Miller²,

Ruddle³

1980

Proc. Natl. Acad. Sci. U.S.A.

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mentioning

confidence: 99%

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Klobutcher¹,

Miller²,

Ruddle³

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…Upon continuous cultivation of unstable transformants, subclones arise that retain the transferred marker stably. Previous data provide strong evidence that in stable transformants the transgenome is integrated into a host chromosome (6)(7)(8)(9).…”

mentioning

confidence: 99%

Molecular analysis of chromosome-mediated gene transfer.

Scangos¹,

Huttner²,

Silverstein³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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“…Transport is followed by a phosphorylation event to a nucleoside monophosphate, with subsequent metabolism and incorporation into macromolecules [8,44,451. Separation of these events in mammalian systems has been reported through the use of thymidine kinase deficient (TK-) mutants [9,11,46,47] , rapid transport studies [8,48 -501, and ATP depletion to prevent phosphorylation [5 11. Actual genetic separation of these events with thymidine transport-deficient (TT-) cells having normal TK activity has been limited to a few studies, including those in Chinese hamster ovary cells DNA (471, viral genome [47] , and chromosome transfer [ 121 has become nearly routine, the reversion of TT-to TT' using genetic material, without affecting TK, has been demonstrated satisfactorily only in the haploid frog cell system [l 1,121 .…”

Section: Discussionmentioning

confidence: 99%

Alteration of human breast tumor cell membrane functions by chromosome‐mediated gene transfer

Muneer

Gray

1979

J Supramol Struct

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BOT-2 cells (human breast tumor origin) have an impaired ability to utilize exogenous thymidine. Previous studies revealed this deficiency to be the permeation event rather than phosphorylation, since the cells have active thymidine kinase. Chromosome-mediated gene transfer was used to transfer genetic information in the form of metaphase chromosomes, from HeLa-65 cells to the BOT-2 cells, correcting the permease deficiency. Poly-L-ornithine or lipochromes were used for facilitation of chromosome uptake. After selection on HAT medium, transferant clones were isolated at a frequency of 4 x 10(-5) and 1 x 10(-5), respectively. Transferants MGP-1 and MGL-1 are stable after 18 months and have been characterized on the bases of purine and pyrimidine nucleoside uptake, relative thymidine kinase activities, alkaline phosphatase activities, and hydrocortisone-induced alkaline phosphatase activity. MGP-1 demonstrates positive thymidine uptake and incorporates radiolabeled thymidine into DNA. MGL-1 remains thymidine transport-deficient and surveys on HAT by increasing endogenous dihydrofolate reductase activity. Alkaline phosphatase activity in MGL-1 is similar to HeLa-65, 2% of that in BOT-2, and in addition, is inducible 25-30-fold by 3 micro M hydrocortisone. We have separated, genetically, a thymidine permease function from phosphorylation in cells of human origin and have transferred genetic information for the regulation of alkaline phosphatase.

show abstract

Chromosomal gene transfer of human cytosol thymidine kinase into mouse cells

Cited by 14 publications

References 33 publications

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Molecular analysis of chromosome-mediated gene transfer.

Alteration of human breast tumor cell membrane functions by chromosome‐mediated gene transfer

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