Chromosomal evolution of neotropical cichlids: the role of repetitive DNA sequences in the organization and structure of karyotype

Schneider, Carlos Henrique; Gross, Maria Cláudia; Terencio, Maria Leandra; Artoni, Roberto Ferreira; Vicari, Marcelo Ricardo; Martins, Cesar; Feldberg, Eliana

doi:10.1007/s11160-012-9285-3

Cited by 42 publications

(69 citation statements)

References 44 publications

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“…The simple 5S rDNA cistrons found in Gymnogeophagus setequedas were located in an interstitial position on the long arm of the subtelocentric chromosome pair 4, which is a similar configuration to that found in other geophagin species, such as Satanoperca jurupari (Nakajima et al, 2012), Geophagus proximus (Schneider et al, 2013) and G. brasiliensis (Vicari et al, 2006;Perazzo et al, 2013), and species from closely-related tribes, such as Cichla monoculus (Cichlini), Astronotus ocellatus (Astronotini) (Schneider et al, 2013), and Australoheros angiru (Heroini) (Paiz et al, 2014). However, these cistrons are found in different locations in other species.…”

Section: Discussionsupporting

confidence: 75%

“…2n = diploid number; m = metacentric; sm = submetacentric; st = subtelocentric; a = acrocentric; p = short arm; q = long arm; t = terminal position; i = interstitial position; c = centromeric position; chr = chromosomes. References: (1) Nakajima et al (2012); (2) Paiz et al (2014); (3) Perazzo et al (2011); (4) Poletto et al (2010a); (5) Poletto et al (2010b); (6) Schneider et al (2013); (7) Vicari et al (2006). Classification used follows Smith et al (2008 …”

Section: Resultsmentioning

confidence: 99%

“…In the remaining species of the Etroplinae, Pseudocrenilabrinae and Cichlinae, analyses have shown simple 18S rDNA cistrons (Poletto et al, 2010a), which vary in their position (terminal or interstitial) and the chromosome pairs (metasubmetacentric or subtelo-acrocentric) that bear them. Cichla monoculus (Cichlini) and Retroculus lapidifer (Retroculini) belong to basal cichline genera (Smith et al, 2008), and differ in the location of the 18S rDNA cistrons, which are found in a terminal position on the long arm of the subtelo-acrocentric chromosomes in C. monoculus (Schneider et al, 2013) and in a terminal position on the short arm of the subtelo-acrocentric chromosomes in R. lapidifer (Poletto et al, 2010a). In Pseudetroplus maculatus (Bloch, 1795) (Etroplinae) and Hemichromis bimaculatus (Pseudocrenilabrinae), considered here as outgroups, simple 18S rDNA cistrons are located on the short arm of the subtelo-acrocentric chromosomes and represent a plesiomorphic character in the Cichlidae (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Previous cytogenetic data are available for species of the genera Geophagus Heckel, 1840 (Vicari et al, 2006;Pires et al, 2010;Nakajima et al, 2012;Schneider et al, 2013;Perazzo et al, 2013), Gymnogeophagus Miranda Ribeiro, 1918 (Pires et al, 2010), Apistogramma Regan, 1913, Biotodoma Eigenmann & Kennedy, 1903and Satanoperca Günther, 1862(Poletto et al, 2010a. In the case of Gymnogeophagus, cytogenetic studies have focused on just three species, G. balzanii (Feldberg, Bertollo, 1984, 1985aRoncati et al, 2007), G. gymnogenys and G. labiatus (Pires et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetics of Gymnogeophagus setequedas (Cichlidae: Geophaginae), with comments on its geographical distribution

et al. 2017

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We provide cytogenetic data for the threatened species Gymnogeophagus setequedas, and the first record of that species collected in the Iguaçu River, within the Iguaçu National Park's area of environmental preservation, which is an unexpected occurrence for that species. We verified a diploid number of 2n = 48 chromosomes (4sm + 24st + 20a) and the presence of heterochromatin in centromeric and pericentromeric regions, which are conserved characters in the Geophagini. The multiple nucleolar organizer regions observed in G. setequedas are considered to be apomorphic characters in the Geophagini, whereas the simple 5S rDNA cistrons located interstitially on the long arm of subtelocentric chromosomes represent a plesiomorphic character. Because G. setequedas is a threatened species that occurs in lotic waters, we recommend the maintenance of undammed environments within its known area of distribution.Keywords: Chromosomes, Conservation, Iguaçu River, Karyotype, Paraná River.Fornecemos dados citogenéticos para a espécie ameaçada Gymnogeophagus setequedas, e o primeiro registro da espécie coletado no rio Iguaçu, na área de preservação ambiental do Parque Nacional do Iguaçu, a qual é uma área de ocorrência inesperada para esta espécie. Verificamos em G. setequedas 2n = 48 cromossomos (4sm + 24st + 20a) e heterocromatina presente nas regiões centroméricas e pericentroméricas, as quais indicam caracteres conservados em Geophagini. Múltiplas regiões organizadoras de nucléolos foram observadas em G. setequedas e são consideradas características apomórficas em Geophagini, enquanto cístrons de DNAr 5S simples e localizados intersticialmente no braço longo de cromossomos subtelocêntricos representam uma característica plesiomórfica. Visto que G. setequedas é uma espécie ameaçada de extinção que ocorre em águas lóticas, recomendamos a manutenção de ambientes livre de barragens em sua área de distribuição.

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Section: Discussionsupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytogenetics of Gymnogeophagus setequedas (Cichlidae: Geophaginae), with comments on its geographical distribution

et al. 2017

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“…4,[28][29][30][31][32] Furthermore, previous studies reported the participation of these sequences in chromosomal rearrangements associated with karyotype diversification events and the differentiation of sex chromosomes. [33][34][35][36][37] New methods of next-generation genomic sequencing generated a large amount of information, providing extensive data related to the DNA sequences of organisms; however, arranging the position of repetitive sequences is extremely difficult. 38 Thus, numerous studies identified and physically mapped repetitive sequences to assist in the organization of chromosomal regions rich in repetitive DNA, particularly in heteromorphic sex chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Construction and Characterization of a Repetitive DNA Library in Parodontidae (Actinopterygii: Characiformes): A Genomic and Evolutionary Approach to the Degeneration of the W Sex Chromosome

et al. 2014

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Repetitive DNA sequences, including tandem and dispersed repeats, comprise a large portion of eukaryotic genomes and are important for gene regulation, sex chromosome differentiation, and karyotype evolution. In Parodontidae, only the repetitive DNAs WAp and pPh2004 and rDNAs were previously studied using fluorescence in situ hybridization. This study aimed to build a library of repetitive DNA in Parodontidae. We isolated 40 clones using C o t-1; 17 of these clones exhibited similarity to repetitive DNA sequences, including satellites, minisatellites, microsatellites, and class I and class II transposable elements (TEs), from Danio rerio and other organisms. The physical mapping of the clones to chromosomes revealed the presence of a satellite DNA, a Helitron element, and degenerate short interspersed element (SINE), long interspersed element (LINE), and tc1-mariner elements on the sex chromosomes. Some clones exhibited dispersed signals; other sequences were not detected. The 5S rDNA was detected on an autosomal pair. These elements likely function in the molecular degeneration of the W chromosome in Parodontidae. Thus, the location of these elements on the chromosomes is important for understanding the function of these repetitive DNAs and for integrative studies with genome sequencing. The presented data demonstrate that an intensive invasion of TEs occurred during W sex chromosome differentiation in the Parodontidae.

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Pericentromeric location of the telomeric DNA sequences on the European grayling chromosomes

et al. 2013

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The chromosomal characteristics, locations and variations of the C-band positive heterochromatin and telomeric DNA sequences were studied in the European grayling karyotype (Thymallus thymallus, Salmonidae) using conventional C-banding, endonucleases digestion banding, silver nitrate (AgNO3), chromomycin A3 and 4′,6-diamidino-2-phenylindole staining techniques as well as fluorescence in situ hybridization (FISH) and primed in situ labelling. Original data on the chromosomal distribution of segments resistant to AluI restriction endonuclease and identification of the C-banded heterochromatin presented here have been used to characterize the grayling karyotype polymorphism. Structural and length polymorphism of the chromosome 21 showing a conspicuous heterochromatin block adjacent to the centromere seems to be the result of the deletion and inversion. Two pairs of nuclear organizer regions (NOR)-bearing chromosomes were found to be polymorphic in size and displaying several distinct forms. FISH with telomeric peptide nucleic acid probe enabled recognition of the conservative telomeric DNA sequences. The karyotype of the thymallid fish is thought to experienced numerous pericentric inversions and internal telomeric sites (ITSs) observed at the pericentromeric regions of the six European grayling metacentric chromosomes are likely relics of the these rearrangements. None of the ITS sites matched either chromosome 21 or NOR bearing chromosomes.

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Chromosomal evolution of neotropical cichlids: the role of repetitive DNA sequences in the organization and structure of karyotype

Cited by 42 publications

References 44 publications

Cytogenetics of Gymnogeophagus setequedas (Cichlidae: Geophaginae), with comments on its geographical distribution

Cytogenetics of Gymnogeophagus setequedas (Cichlidae: Geophaginae), with comments on its geographical distribution

Construction and Characterization of a Repetitive DNA Library in Parodontidae (Actinopterygii: Characiformes): A Genomic and Evolutionary Approach to the Degeneration of the W Sex Chromosome

Pericentromeric location of the telomeric DNA sequences on the European grayling chromosomes

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