CHROMOSOMAL EVOLUTION IN
            <i>HAPLOPAPPUS GRACILIS</i>
            : A CENTRIC TRANSPOSITION RACE

Chromosome numbers are reported for 13 species and four artificially produced interspecific hybrids of Fuchsia (Onagraceae). The chromosome number of F. encliandra is reported here for the first time and new chromosome numbers were obtained for F. triphylla and F. boliviana. an approximately 2.4-fold variation in DNa C-value was observed with a range of values from 1.46 pg to 3.44 pg per 2C nucleus. The lowest C-values were found in the species from New Zealand. The four polyploidy species had considerably lower 1Cx-values than most of the diploid species. The nuclear DNA content of the hybrids was, as expected, intermediate between those of the parental species.

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“…Chromosome numbers of all species and hybrids were determined using the Feulgen method and squashing the root tips in FlP orcein (Jackson 1973).…”

Section: Methodsmentioning

confidence: 99%

DNA C‐values and chromosome numbers inFuchsiaL. (Onagraceae) species and artificial hybrids

Talluri

Murray

2009

New Zealand Journal of Botany

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“…The roots were hydrolysed in 1 M HCl at 60 8C for 6Á8 min, transferred to 45% acetic acid and then macerated on a slide in a drop of FLP orcein (Jackson 1973), heated over a spirit flame, flattened under a coverslip and observed under the microscope. Meiotic chromosomes were obtained from immature anthers that were fixed in ethanol/chloroform/acetic acid (6:3:1) and stained with FLP orcein as above.…”

Section: Methodsmentioning

confidence: 99%

New chromosome counts in New Zealand species ofPlantago(Plantaginaceae)

Murray

Meudt

Tay

et al. 2010

New Zealand Journal of Botany

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Polyploidy is common in the widespread genus Plantago, and might be especially important in the evolutionary history of native New Zealand species of the genus. To further understanding of native New Zealand Plantago, mitotic and meiotic chromosome counts are reported from 58 individuals representing most native species and subspecies, and one introduced species, complementing previous studies by extending the number of individuals and the geographic range of taxa counted. Previous counts were confirmed for most Plantago lanigera (2n 0 2x012), all P. raoulii, P. spathulata subsp. spathulata, P. spathulata subsp. picta, P. triandra subsp. triandra and P. triandra subsp. masoniae (2n 0 8x 0 48), most P. unibracteata (2n 0 10x 0 60), and all P. sp. 'Sylvester' (2n 0 16x 0 96). Novel counts include 2n 0 12x 0 72 for four individuals identified as P. unibracteata, and 2n 0 48 for four individuals from Sugarloaf Pass, South Island, referred to here as P. aff. spathulata. One of the eight naturalized species, P. major, was diploid with 2n 0 12. These results, together with other studies, suggest that all native New Zealand Plantago have a base chromosome number of x 0 6 and most polyploids are allopolyploids.

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“…In most intsances, a minimum of 500 grains was scored from each slide prepared from each of three floret samples per head. Pollen which stained a solid deep purple was scored as stained while pollen which was mottled or light purple in color was scored as unstained following Jackson (1973) and Jackson and Jordan (1975). The number of micropollen grains was recorded.…”

Section: Methodsmentioning

confidence: 99%