Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa

Le, Shuai; Yao, Xinyue; Lu, Shuguang; Tan, Yinling; Rao, Xiancai; Li, Ming; Jin, Xiaolin; Wang, Jing; Zhao, Yan; Wu, Nicholas C.; Lux, Renate; He, Xuesong; Shi, Wenyuan; Hu, Fuquan

doi:10.1038/srep04738

Cited by 92 publications

(101 citation statements)

References 53 publications

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“…We and others showed that large chromosomal deletions could be selected in bacteria resistant to single or multiple phages. Such deletions encompassed genes involved in fimbriae, outer membrane proteins or LPS components (Latino et al, 2014;Le et al, 2014;Tanji et al, 2008). Resistance to phages due to modified type IV pili has consequences for bacterial motility by affecting twitching -a form of solid surface translocation (Chiang & Burrows, 2003).…”

Section: Introductionmentioning

confidence: 99%

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Latino

2016

Microbiology

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Coevolution between bacteriophages (phages) and their prey is the result of mutualistic interactions. Here, we show that pseudolysogeny is a frequent outcome of infection by virulent phages of Pseudomonas aeruginosa and that selection of resistant bacterial mutants is favoured by continuous production of phages. We investigated the frequency and characteristics of P. aeruginosa strain PAO1 variants resisting infection by different combinations of virulent phages belonging to four genera. The frequency of resistant bacteria was 10 25 for single phage infection and 10 26 for infections with combinations of two or four phages. The genome of 27 variants was sequenced and the comparison with the genome of the parental PAO1 strain allowed the identification of point mutations or small indels. Four additional variants were characterized by a candidate gene approach. In total, 27 independent mutations were observed affecting 14 genes and a regulatory region. The mutations affected genes involved in biosynthesis of type IV pilus, alginate, LPS and O-antigen. Half of the variants possessed changes in homopolymer tracts responsible for frameshift mutations and these phase variation mutants were shown to be unstable. Eleven double mutants were detected. The presence of free phage DNA was observed in association with exclusion of superinfection in half of the variants and no chromosomal mutation could be found in three of them. Upon further growth of these pseudolysogens, some variants with new chromosomal mutations were recovered, presumably due to continuous evolutionary pressure.

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Section: Introductionmentioning

confidence: 99%

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Latino

2016

Microbiology

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“…While it is unclear how these compounds interfered with the pigmentation of P. aeruginosa , mutations in pigmentation genes such as pyocyanin, have been linked to antibiotic resistance and resistance to phages [18]. Given the apparent importance of P. aeruginosa pigmentation the inhibition observed should be further studied.…”

Section: Discussionmentioning

confidence: 99%

Para-Substituted Functionalised Ferrocene Esters with Novel Antibacterial Properties

Forti

Bernard

Santiago-Collazo

et al. 2018

JCDR

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Introduction Bacterial antibiotic resistance is on rise despite advances in the development of new antibiotics. In an attempt to circumvent resistance, scientists are shifting focus from modifying existent antibiotics to identifying new antibiotic compounds. Aim To assess the potential antibiotic effects of functionalised ferrocenecarboxylates para-substituted on the phenoxy pendant group to form: 4-fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-iodophenyl and 4-(H-pyrrol-1-yl)phenyl. Materials and Methods For this, we employed the Kirby-Bauer disc diffusion method using a collection of nine bacterial species: Staphylococcus aureus, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Serratia marcescens, Klebsiella pneumoniae, Bacillus subtilis, Proteus vulgaris and Enterobacter aerogenes. Results The results show that all four-halogen substituted ferrocenecarboxylates 4-fluorophenyl (23.33 μM, 11.66 μM, 5.83 μM), 4-chlorophenyl (10.16 μM, 5.08 μM, 2.54 μM), 4-bromophenyl (9.0 μM, 4.5 μM, 2.25 μM), and 4-iodophenyl (17.12 μM, 8.56 μM, 4.28 μM) exhibited an antibacterial effect by reducing proliferation of Bacillus subtilis. Meanwhile, only 4-bromophenyl (9.0 μM) and 4-chlorophenyl (10.16 μM) ferrocenecarboxylates were able to decrease the growth of Micrococcus luteus. Conclusion Hence, functionalised ferrocenecarboxylates para-substituted with small and simple groups represent a novel class of bio-organometallic compounds with the potential to be used as antibacterial agents.

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“…This impressive work, beginning with bioinformatic analyses and, followed by bacteriophage plaque assays and proteinprotein interaction studies, not only identified and functionally characterized new toxin and antitoxin proteins, but also identified a new protein counteracting bacterial defenses in the T7 bacteriophage. Additionally, an original way for bacteria to resist viral infections has just been reported [16]. By a yet unknown mechanism, a large chromosomal deletion (200 kb) led to the loss of at least one gene required for the synthesis of bacteriophage receptor.…”

Section: Current Opinion In Microbiologymentioning

confidence: 98%

Bacterial sensing of bacteriophages in communities: the search for the Rosetta stone

Debarbieux

2014

Current Opinion in Microbiology

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Billions of years of evolution have resulted in microbial viruses and their hosts communicating in such a way that neither of these antagonists can dominate the other definitively. Studies of the molecular mechanisms underlying this dialog, initially in bacteriophages, rapidly identified several of the ways in which bacteria resist bacteriophage infections and bacteriophages defeat bacterial defenses. From an ecological perspective, recent data have raised many questions about the dynamic interactions between bacteria and bacteriophages, the densities of which, in complex microbial populations, are only beginning to be investigated. The next challenge will be determining how the dialog between microbial viruses and their hosts modulates complex ecosystems, such as those found in healthy humans or infected patients.

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Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa

Cited by 92 publications

References 53 publications

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Para-Substituted Functionalised Ferrocene Esters with Novel Antibacterial Properties

Bacterial sensing of bacteriophages in communities: the search for the Rosetta stone

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