Chromosomal deletion or rearrangement in chimeric hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus obtained by cell fusion

Kishida, Masao; Muguruma, Tomoaki; Sakanaka, Kazunobu; Katsuragi, Tohoru; Sakai, Taku

doi:10.1016/0922-338x(96)80577-0

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…These parental strains can be from the same species, but often a Saccharomyces strain is combined with a nonconventional yeast displaying a specific trait, such as lactose utilization (Taya et al ., 1984; Farahnak et al ., 1986; Krishnamoorthy et al ., 2010; Guo et al ., 2012), temperature tolerance (Sakanaka et al ., 1996), osmotolerance (Spencer et al ., 1985; Loray et al ., 1995; Lucca et al ., 2002), starch degradation (Kishida et al ., 1996), killer activity (Gunge & Sakaguchi, 1981), malic acid degradation (Carrau et al ., 1994), or (hemi)cellulose hydrolysate utilization (Pina et al ., 1986; Heluane et al ., 1993; Table1). …”

Section: Natural and Artificial Diversitymentioning

confidence: 99%

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels¹,

Snoek²,

Meersman³

et al. 2014

FEMS Microbiol Rev

397

288

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Yeasts have been used for thousands of years to make fermented foods and beverages, such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or species for a specific industrial application is often based on historical, rather than scientific grounds. Moreover, new biotechnological yeast applications, such as the production of second-generation biofuels, confront yeast with environments and challenges that differ from those encountered in traditional food fermentations. Together, this implies that there are interesting opportunities to isolate or generate yeast variants that perform better than the currently used strains. Here, we discuss the different strategies of strain selection and improvement available for both conventional and nonconventional yeasts. Exploiting the existing natural diversity and using techniques such as mutagenesis, protoplast fusion, breeding, genome shuffling and directed evolution to generate artificial diversity, or the use of genetic modification strategies to alter traits in a more targeted way, have led to the selection of superior industrial yeasts. Furthermore, recent technological advances allowed the development of high-throughput techniques, such as ‘global transcription machinery engineering’ (gTME), to induce genetic variation, providing a new source of yeast genetic diversity.

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Section: Natural and Artificial Diversitymentioning

confidence: 99%

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels¹,

Snoek²,

Meersman³

et al. 2014

FEMS Microbiol Rev

397

288

View full text Add to dashboard Cite

show abstract

“…For example, S. cerevisiae is able to form hybrids with Lindnera jadinii (34,163), Kuraishia capsulata (205,206), Saccharomycopsis fibuligera (58,97), and Pachysolen tannophilus (44,80), four species that belong to yet other families of the Saccharomycotina (101). Even broader evolutionary distances exist between Yarrowia lipolytica and K. lactis, Lindnera jadinii, Meyerozyma guilliermondii, and Saccharomycopsis fibuligera, respectively, with which it forms hybrids (74,84,150,203).…”

Section: Artificial Hybrids Made By Protoplast Fusion Of Other Yeastsmentioning

confidence: 99%

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Morales

Dujon

2012

Microbiol Mol Biol Rev

153

161

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SUMMARYForced interspecific hybridization has been used in yeasts for many years to study speciation or to construct artificial strains with novel fermentative and metabolic properties. Recent genome analyses indicate that natural hybrids are also generated spontaneously between yeasts belonging to distinct species, creating lineages with novel phenotypes, varied genetic stability, or altered virulence in the case of pathogens. Large segmental introgressions from evolutionarily distant species are also visible in some yeast genomes, suggesting that interspecific genetic exchanges occur during evolution. The origin of this phenomenon remains unclear, but it is likely based on weak prezygotic barriers, limited Dobzhansky-Muller (DM) incompatibilities, and rapid clonal expansions. Newly formed interspecies hybrids suffer rapid changes in the genetic contribution of each parent, including chromosome loss or aneuploidy, translocations, and loss of heterozygosity, that, except in a few recently studied cases, remain to be characterized more precisely at the genomic level by use of modern technologies. We review here known cases of natural or artificially formed interspecies hybrids between yeasts and discuss their potential importance in terms of genome evolution. Problems of meiotic fertility, ploidy constraint, gene and gene product compatibility, and nucleomitochondrial interactions are discussed and placed in the context of other known mechanisms of yeast genome evolution as a model for eukaryotes.

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“…Protoplasts with both kinds of fluorescence were selected with a FACS, and colonies were grown on osmotic plates [16]. Strains obtained as regenerated colonies were seen to be true hybrids when chromosomal analysis was done by electrophoresis [17]. Similar results have been obtained from the mating of different strains of Saccharomyces cerevisiae followed by use of FACS to obtain hybrids [18].…”

Section: Single-cell Selection Of Yeast Hybrids Without Use Of Genetimentioning

confidence: 52%

Single-Cell Sorting of Microorganisms by Flow or Slide-Based (Including Laser Scanning) Cytometry

Katsuragi

Tani

2001

Acta Biotechnol.

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In applied microbiology, strain improvement of microorganisms by conventional selection culture is not always successful, so single‐cell selection of viable cells with the desired characteristics from a large heterogeneous population may be used instead. Single‐cell selection with use of a micromanipulator is possible, but laborious. For many applications, the process has been automated. In this review, an automated method, laser scanning cytometry (LSC), is outlined together with flow cytometry (FCM). FCM is familiar to many microbiologists, but LSC is a microscopic‐slide‐based method that is less well known. One of its advantages is its possible use in the examination of small cell populations.In addition, individual cells can be examined repeatedly, measured automatically and later observed microscopically by the operator, and finally stored (if desired)on the microscopic slide on which they are placed. Fluorescent and other probes are available in abundance for FCM, and almost all might be used in LSC. A number of applications of these methods are cited from the extensive literature (mostly about FCM), but the list of possible applications in this review is far from being exhaustive. This review is intended as an introduction for the applied microbiologist to the manifold uses of LSC.

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Chromosomal deletion or rearrangement in chimeric hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus obtained by cell fusion

Cited by 9 publications

References 10 publications

Improving industrial yeast strains: exploiting natural and artificial diversity

Improving industrial yeast strains: exploiting natural and artificial diversity

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Single-Cell Sorting of Microorganisms by Flow or Slide-Based (Including Laser Scanning) Cytometry

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